| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,927,062 | +G | coding (2/1284 nt) coding (169/183 nt) |
yieM ← b4564 ← |
hypothetical protein hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,927,062 | 1 | . | G | 99.1% | 304.2 / NA | 115 | M1A (GTG→GCG) † C57R (TGC→CGC) | yieM b4564 | hypothetical protein hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (59/55); minor base T (0/1); total (59/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
AGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCA‑CGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGA > NC_000913/3926987‑3927130 | agatGGCTTTGTTGATAGCACATCACCTCTTCGGTGAGTTCGGGCGGGACACGGGCATCTTTCAGCCGACTGCGCa < 2:2734512/73‑1 (MQ=255)agCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCa < 1:1210314/76‑1 (MQ=255)agCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCa < 2:4418158/76‑1 (MQ=255) cTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCagc < 2:3706612/76‑3 (MQ=255) tGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCagcg > 1:2939087/1‑73 (MQ=255) tGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCagcg > 1:2151408/1‑73 (MQ=255) tGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCagcg < 2:1858738/76‑4 (MQ=255) tttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTc < 1:784653/76‑1 (MQ=255) tttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTc > 1:4613339/1‑76 (MQ=255) tttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTc > 1:4066584/1‑76 (MQ=255) ttGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCa < 2:2745732/76‑1 (MQ=255) tGCTGATAGCACATCGCCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCAc < 2:4157915/76‑1 (MQ=255) tGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTccc > 2:4203467/1‑74 (MQ=255) tGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCAc > 2:1104055/1‑76 (MQ=255) tGCTGATAGCACATCACCTCTTCGGTGAGCTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCAc < 1:4334984/76‑1 (MQ=255) cTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGGCTGCGCAGCGCTTCACGc < 2:3061243/76‑1 (MQ=255) cTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCCCGc > 2:156836/1‑76 (MQ=255) cTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGc < 1:4698891/76‑1 (MQ=255) tGATAGCACTTCACCTCTTGGGTGGGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGcc < 2:761277/76‑1 (MQ=255) tGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGcc < 1:1709363/76‑1 (MQ=255) aTAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAg < 2:526420/76‑1 (MQ=255) aTAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAg < 1:2459159/76‑1 (MQ=255) tAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc < 2:4181636/76‑1 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc > 1:2690752/1‑76 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc < 2:4139955/76‑1 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc < 2:2866702/76‑1 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc < 1:4801220/76‑1 (MQ=255) tAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGc < 1:3561872/76‑1 (MQ=255) aGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCg > 1:4089567/1‑76 (MQ=255) gcgcaTCACCTCTTCGGTGAGTTCCGGCGGGACTAGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgg < 2:2350375/73‑1 (MQ=255) gCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgg < 1:2797115/76‑1 (MQ=255) acaTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgggg < 1:668902/76‑1 (MQ=255) acaTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgggg > 1:1951967/1‑76 (MQ=255) acaTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgggg > 1:2583475/1‑76 (MQ=255) acaTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACCACAGCgcgg > 2:3522550/1‑76 (MQ=255) acaTCACCTCGTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCgggg < 2:1533656/76‑1 (MQ=255) aTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGaa > 1:3406825/1‑76 (MQ=255) aTCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGaa > 2:514430/1‑76 (MQ=255) tCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAc < 1:2477597/76‑1 (MQ=255) tCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAc < 1:2512154/76‑1 (MQ=255) cACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGACca > 2:65687/1‑76 (MQ=255) cACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAca > 2:1209976/1‑76 (MQ=255) cACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAca < 1:4562180/76‑1 (MQ=255) ccTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcatc > 1:4862966/1‑76 (MQ=255) ctctTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcatca > 1:1642209/1‑76 (MQ=255) ctctTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcatca > 1:1456951/1‑76 (MQ=255) tctTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcatcat > 1:4020329/1‑76 (MQ=255) ctTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACatcatc > 2:449989/1‑76 (MQ=255) ttCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACAtcatca > 1:374507/1‑76 (MQ=255) tgcGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACAtcatca < 2:2584653/74‑1 (MQ=255) ggTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTg > 2:3162056/1‑76 (MQ=255) ggTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTg > 2:3198417/1‑76 (MQ=255) ggTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGCAACATCATCACTg > 2:3015946/1‑76 (MQ=255) tGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGAt > 2:2012207/1‑76 (MQ=255) gAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGAtt > 2:2486262/1‑76 (MQ=255) aGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTg < 1:4270425/76‑1 (MQ=255) aGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTg > 1:1426542/1‑76 (MQ=255) gTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgc < 2:1029428/76‑1 (MQ=255) gTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgc > 2:886604/1‑76 (MQ=255) ttCCGGCGGGCCTCGGGCATCTTTCAGCCGACTGCGCCGCGCTCCACGCCAGCGGGGACCATCACCAGTGATTgcc > 1:4464056/1‑76 (MQ=255) ttCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgcc < 1:834271/76‑1 (MQ=255) tCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccg > 2:2084408/1‑76 (MQ=255) tCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccc > 2:981142/1‑75 (MQ=255) ccGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgc > 2:1872803/1‑76 (MQ=255) ccGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgc < 1:791883/76‑1 (MQ=255) ccGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgc > 2:4293178/1‑76 (MQ=255) ccGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgc < 1:4639504/76‑1 (MQ=255) cggcggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgcc > 1:2898145/1‑76 (MQ=255) cggcggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgccgcc > 1:2124612/1‑76 (MQ=255) ggcggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCt > 1:2150809/1‑76 (MQ=255) ggcggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCt > 1:3695798/1‑76 (MQ=255) ggcggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGCGATTGCCGCCt > 2:757614/1‑76 (MQ=255) gcggGCCGCGGGCATCTTTCAGCCGACTGCGCAGCGCTGCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCtt < 1:3803932/76‑1 (MQ=255) tgggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTc > 2:3729047/2‑76 (MQ=255) cggGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTc < 1:2320735/76‑1 (MQ=255) agggAATCGGGCATCTTTCACCAGAAAGCCCAGCGCTTCACGACAGCAGCGAACATCATCAGTAATTGACGCCTTc > 1:581554/2‑76 (MQ=255) gggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCa < 1:2441597/76‑1 (MQ=255) gggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCa < 2:3331346/76‑1 (MQ=255) ggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAg < 1:2796115/76‑1 (MQ=255) ggACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAg < 1:1454903/76‑1 (MQ=255) gccTCGGGCATCTTTCAGCCGCCTGCGCAGCGCTTCACGCCCGCGGGGAACATCATCAGTGCTTGCCGCCTTCAgg > 1:2608757/3‑76 (MQ=255) gACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAgg > 1:3274327/1‑76 (MQ=255) gACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAgg > 1:2576033/1‑76 (MQ=255) aCTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGc < 2:2838859/76‑1 (MQ=255) aCTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGc < 1:2182206/76‑1 (MQ=255) cTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCg > 2:2615698/1‑76 (MQ=255) tCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGt > 2:2510261/1‑76 (MQ=255) cGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTg < 2:489153/76‑1 (MQ=255) gggCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTgg > 1:1611138/1‑76 (MQ=255) cATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGaa > 2:412105/1‑76 (MQ=255) aTCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCGGGCGTGGGAAt < 2:4200910/76‑1 (MQ=255) tCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAtt > 1:322470/1‑76 (MQ=255) tCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTc < 1:96613/76‑1 (MQ=255) gCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCaaa > 1:354965/1‑76 (MQ=255) ccGCCTGCGCAGCGCTTCGCGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaag < 1:4574279/76‑1 (MQ=255) ccGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaag < 1:4573482/76‑1 (MQ=255) ccGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaag < 2:2163023/76‑1 (MQ=255) cGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaaga > 2:373555/1‑76 (MQ=255) gACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaagaa < 1:1292660/76‑1 (MQ=255) aCTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaagaag < 2:2251172/76‑1 (MQ=255) | AGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCA‑CGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGA > NC_000913/3926987‑3927130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |