| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,957,124 | +G | intergenic (+58/+26) | ilvC → / ← ppiC | ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,957,123 | 1 | . | G | 97.5% | 181.1 / ‑1.3 | 81 | intergenic (+57/+27) | ilvC/ppiC | ketol‑acid reductoisomerase/peptidyl‑prolyl cis‑trans isomerase C |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (35/44); total (37/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGA‑‑CTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGG > NC_000913/3957051‑3957193 | tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑g > 1:4414096/1‑76 (MQ=18) gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑gg < 2:2305205/76‑2 (MQ=17) gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑gg < 2:3756495/76‑2 (MQ=17) gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑gg > 1:2133084/1‑75 (MQ=17) cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑ggc < 1:4433576/76‑3 (MQ=17) tGTTGCGGGTTAAGGGCGCGCTGATGCCCTCACCCCGACCCGCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑ggcc > 1:703250/1‑73 (MQ=17) tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtc > 2:1941298/1‑76 (MQ=14) tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtc < 2:2925065/76‑1 (MQ=14) gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtct < 2:1612000/76‑1 (MQ=14) gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtct < 2:2368892/76‑1 (MQ=14) gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtct > 2:2722957/1‑76 (MQ=14) gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtct > 1:955500/1‑76 (MQ=14) cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtctc > 1:2074472/1‑76 (MQ=14) cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTtctc < 1:2565759/76‑1 (MQ=14) gggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTcc > 1:1461407/1‑76 (MQ=14) gggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTcc > 1:2235355/1‑76 (MQ=14) gggTTAAGTGCGCGCTGATGACCTCACACCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTcc > 1:901981/1‑76 (MQ=14) ggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCt < 2:3257407/76‑1 (MQ=14) ggTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCTCCCACAGGGAGAGGGAGCACACACT‑C‑AAGGCCTTCTCCt < 2:703250/76‑1 (MQ=14) tAAGTGCGCGCTGATGCCCTCTCCCCGACCCTCTCCCACAGGTAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGga < 1:2589307/76‑1 (MQ=14) aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCC‑AG‑GCCTTCTCCTGgaga > 1:3318988/1‑76 (MQ=14) gTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGaa < 2:4635747/76‑1 (MQ=14) gTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGaa < 2:3931756/76‑1 (MQ=14) gTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGaa < 2:426167/76‑1 (MQ=14) tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAg < 2:1289684/76‑1 (MQ=14) tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAg < 1:1973819/76‑1 (MQ=17) tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCCTCTCCTGGAGAAg > 2:990502/1‑76 (MQ=14) ggcgcgcTGATGCCCTACCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAg < 2:2198243/75‑1 (MQ=14) cgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGCAGGc > 2:1746703/1‑76 (MQ=14) gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑CAAA‑GCCTGCTCCTGGAGAAGGcc > 1:883833/1‑76 (MQ=17) gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGcc > 1:3941187/1‑76 (MQ=17) gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGcc > 2:1309370/1‑76 (MQ=17) cgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCt > 1:1166489/1‑76 (MQ=17) tcggATGCCCTGACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCtt < 1:700137/73‑1 (MQ=17) gcTGATGCCCTCACCGCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCtt < 1:956227/76‑1 (MQ=17) gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCt < 1:4145504/76‑1 (MQ=18) gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCt < 2:832927/76‑1 (MQ=18) gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCt > 1:1322609/1‑76 (MQ=18) aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTa < 2:2774749/76‑1 (MQ=18) gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt < 2:1682834/76‑1 (MQ=18) ccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAg > 1:3385060/1‑76 (MQ=21) cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGt > 1:3049342/1‑76 (MQ=21) tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGtt > 2:4365379/1‑76 (MQ=21) tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGtt > 1:1918893/1‑76 (MQ=21) ccACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGtt < 2:684935/75‑1 (MQ=21) cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg > 1:1400570/1‑76 (MQ=25) accctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa < 1:1192640/73‑1 (MQ=38) accctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa < 1:3965145/73‑1 (MQ=38) accctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa > 1:2191565/4‑76 (MQ=38) ccctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg > 2:1669039/3‑76 (MQ=38) ccctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg < 1:2266195/74‑1 (MQ=38) cctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc > 2:3087169/2‑76 (MQ=255) cctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc < 2:1751870/75‑1 (MQ=255) ctctCCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGcc > 2:1483754/1‑75 (MQ=255) tctcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc < 1:2320381/76‑1 (MQ=255) gctcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc < 2:2296007/75‑1 (MQ=255) ctcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAcc > 2:3458080/1‑76 (MQ=255) ctcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAcc < 1:1941299/76‑1 (MQ=255) tcCCACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt < 2:4224151/76‑1 (MQ=255) ccACAGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTa > 2:1012290/1‑76 (MQ=255) acaGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat < 1:3849552/76‑1 (MQ=255) acaGGGAGAGGGAGAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat > 2:1615497/1‑76 (MQ=255) agggagagggagAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatga > 2:365944/1‑76 (MQ=255) agggagagggagAAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGGACAGCACCTTAatga > 2:726576/1‑76 (MQ=255) gggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgat < 2:1380563/76‑1 (MQ=255) ggagagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg > 1:3444256/1‑76 (MQ=255) gagggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa < 2:4041260/76‑1 (MQ=255) agggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat < 2:2319532/76‑1 (MQ=255) agggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat < 2:2467262/76‑1 (MQ=255) agggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat > 1:1962798/1‑76 (MQ=255) gggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata > 1:1984821/1‑76 (MQ=255) gggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata < 2:2877736/76‑1 (MQ=255) ggagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGatat < 2:3299150/76‑1 (MQ=255) gagaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc < 1:1509860/76‑1 (MQ=255) agaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc < 2:2263968/76‑1 (MQ=255) agaAAACACT‑C‑AAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc < 1:3748113/76‑1 (MQ=255) gaAGACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg < 1:2122368/76‑1 (MQ=255) gaAAACACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCg > 1:586364/1‑76 (MQ=255) aaaCACT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGaa < 1:1341982/76‑1 (MQ=255) acacT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACt > 2:2441170/1‑76 (MQ=255) cacT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg < 2:1339943/76‑1 (MQ=255) acT‑C‑AAGGCCTTCTCCTGGCGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg < 2:3280593/76‑1 (MQ=255) acT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg > 2:3154763/1‑76 (MQ=255) acT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg > 2:2475598/1‑76 (MQ=255) acT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg < 2:1918892/76‑1 (MQ=255) cT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg < 1:4814252/76‑1 (MQ=255) cT‑C‑AAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAAAGCACCTTAATGATGTGATATCCGAACTggg > 2:182386/1‑76 (MQ=255) | TGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGA‑‑CTCTCCCACAGGGAGAGGGAGAAAACACT‑C‑AA‑GCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGG > NC_000913/3957051‑3957193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |