| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,004,984 | +CG | coding (1516/1833 nt) | recQ → | ATP‑dependent DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,004,983 | 1 | . | G | 97.8% | 228.0 / ‑3.0 | 89 | A505A (GCG→GCC) | recQ | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (50/37); minor base C (2/0); total (52/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
| * | NC_000913 | 4,004,983 | 2 | . | C | 97.8% | 228.9 / ‑0.2 | 89 | coding (1515/1833 nt) | recQ | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (50/37); total (52/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑CG‑CG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA > NC_000913/4004911‑4005042 || cGGGCTGGTGACGCAAAATATTGCCCAGCATGCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑Cg‑‑gc < 2:360751/76‑2 (MQ=255)cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑Cg‑‑gc < 2:3431158/76‑2 (MQ=255) ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑Cg‑‑gcg < 1:3877596/76‑3 (MQ=255) gcggCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCctct < 2:251432/73‑1 (MQ=255) gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCctct > 1:3993385/1‑76 (MQ=255) aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCtt < 2:4299271/76‑1 (MQ=255) cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCttt < 2:879175/76‑1 (MQ=255) cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCttt > 2:4400619/1‑76 (MQ=255) cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCttt < 1:2340450/76‑1 (MQ=255) cGCAAAATATTGCCCAGCATCCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCttt < 1:1954907/76‑1 (MQ=255) gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTg > 1:3747828/1‑76 (MQ=255) gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTg > 2:3822628/1‑76 (MQ=255) gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTg > 1:3660345/1‑76 (MQ=255) gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTg < 1:1406360/76‑1 (MQ=255) gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTg > 1:1891309/1‑76 (MQ=255) gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTg < 2:566997/76‑1 (MQ=255) cAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGc > 1:1786731/1‑76 (MQ=255) agaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCa > 1:1898600/3‑76 (MQ=255) aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCa > 1:2191795/1‑76 (MQ=255) atgttGCGCAGCATTCTGCGCTCCCACGGACGGAGGCCGCGCGCCCGGGGC‑TGCG‑CGGCGAATCCTCTTTGCAACt < 2:3994437/73‑1 (MQ=255) atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACt < 2:4482721/76‑1 (MQ=255) tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACtt < 2:2967839/76‑1 (MQ=255) tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACtt > 2:2455634/1‑76 (MQ=255) tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACtt < 2:1889380/76‑1 (MQ=255) tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACtt > 2:2120938/1‑76 (MQ=255) tatTGCCCAGCATTCGGCCCTACAACTGACAGAGGCCGCGCGACCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACtt > 1:3182386/1‑76 (MQ=255) atggCCCAGCATTCTGCCCTACAACTGGCAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtg < 2:249620/73‑1 (MQ=255) atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtg > 1:4149781/1‑76 (MQ=255) tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgcc > 2:298070/1‑76 (MQ=255) tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgcc > 2:4577906/1‑76 (MQ=255) gCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgccg < 2:3500197/76‑1 (MQ=255) cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgccgt < 2:2838792/76‑1 (MQ=255) cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgccgt > 2:3048800/1‑76 (MQ=255) cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgccgt > 2:3052208/1‑76 (MQ=255) cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgccgtgc > 1:1069943/1‑76 (MQ=255) aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgccgtgcc < 1:2379308/76‑1 (MQ=255) cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTtgccgtgcccc > 2:3532228/1‑74 (MQ=255) cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCcgc < 2:2836003/76‑1 (MQ=255) cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCcgc > 1:1643138/1‑76 (MQ=255) aTTCTGCCCTACAGCTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCcgcg < 2:3720050/76‑1 (MQ=255) aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCcgcg > 2:2052186/1‑76 (MQ=255) aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCcgcg > 1:848132/1‑76 (MQ=255) ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGt > 1:368432/1‑76 (MQ=255) ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGt > 1:3613339/1‑76 (MQ=255) tCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGtc > 1:1499711/1‑75 (MQ=255) tCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa > 1:79260/1‑76 (MQ=255) tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCGCCGC‑GAATGCTCTTTGCAACTTGCCGTGCCGCGtctc > 1:4269513/1‑76 (MQ=255) cccTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt < 1:4000300/76‑1 (MQ=255) cccTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt < 2:1586783/76‑1 (MQ=255) ccTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg < 2:2619070/76‑1 (MQ=255) ccTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg < 2:2150769/76‑1 (MQ=255) cTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTCTCGTgg > 2:1421115/1‑76 (MQ=255) cTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg < 1:3436026/76‑1 (MQ=255) cTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg > 1:4518850/1‑76 (MQ=255) tACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc < 1:298070/76‑1 (MQ=255) aCAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCgcggcg > 2:1134173/1‑76 (MQ=255) cAACTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc > 2:889597/1‑76 (MQ=255) aaCTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt > 1:257607/1‑76 (MQ=255) aaCTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt < 2:1013549/76‑1 (MQ=255) aaCTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt > 2:3644912/1‑76 (MQ=255) aCTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACCTGCCGTGCCGCGTATCGTGCCGCTc > 2:612722/1‑76 (MQ=255) cTGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa > 1:2900668/1‑76 (MQ=255) tGACAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa > 2:2482646/1‑76 (MQ=255) gagagagGCCGCGCGTCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGTCGCGTATCGTGGCGCTCaaa < 2:4053771/73‑1 (MQ=255) gcggagGCCGCGCGCCCGGTGCGG‑CG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc < 2:4246228/73‑1 (MQ=255) aCAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc < 1:300051/76‑1 (MQ=255) tagagGCCCCGCGCCCGCTGC‑TGCGCCGC‑GAATCCTCTTTGCAACTTGCCGCACCGCGTATCGCGGCGCTCAAAcc > 2:3812501/2‑76 (MQ=255) cAGAGGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc < 2:1891308/76‑1 (MQ=255) agGCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa < 1:3768866/76‑1 (MQ=255) agGCCGCGAGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCATACCGaa > 1:756549/1‑76 (MQ=255) ggCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCACACCGaaa > 2:4081858/1‑76 (MQ=255) ggCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa > 2:2431776/1‑76 (MQ=255) ggCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa > 1:4847733/1‑76 (MQ=255) ggCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGNATCGTGGCGCTCAAACCGaaa < 1:1879398/76‑1 (MQ=255) cgCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa < 1:2408859/75‑1 (MQ=255) gCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg > 1:1703592/1‑76 (MQ=255) gCCGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg > 1:2873375/1‑76 (MQ=255) ccGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGCAAGc > 1:332696/1‑76 (MQ=255) ccGCGCGCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc < 2:2348738/76‑1 (MQ=255) cgcgcgCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg > 1:1066024/1‑76 (MQ=255) cgcgcgCCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg > 1:85647/1‑76 (MQ=255) gcgcgcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa > 1:4848016/1‑76 (MQ=255) gcgcgcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa > 1:2530357/1‑76 (MQ=255) cgcgcCCGGGGCGT‑CG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCGGCGTATCGTGGCGCTCAAACCGAAAGCGAt < 2:2377989/76‑1 (MQ=255) gcgcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATg > 1:4446691/1‑76 (MQ=255) cgcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc < 1:4679961/76‑1 (MQ=255) cgcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCCAAAGCGATGc > 2:4477923/1‑76 (MQ=255) gcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAGCTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa < 1:2890481/76‑1 (MQ=255) gcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCg > 2:4569291/1‑65 (MQ=255) gcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCg < 1:4569291/65‑1 (MQ=255) gcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa > 1:521360/1‑76 (MQ=255) gcCCGGTGC‑TGCG‑CGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa < 1:2301068/76‑1 (MQ=255) || CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑CG‑CG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA > NC_000913/4004911‑4005042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |