Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,007,907 +GG intergenic (+109/‑11) pldB → / → b4555 lysophospholipase L(2)/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,007,9071.G87.0% 215.0 / 35.5 92intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (8/4);  new base G (44/36);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
*NC_0009134,007,9072.G87.0% 213.8 / 35.5 92intergenic (+109/‑11)pldB/b4555lysophospholipase L(2)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (8/4);  new base G (44/36);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 103 total aligned reads displayed.

AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA  >  NC_000913/4007832‑4007978
                                                                                ||                                                                       
aGATGGTACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgc                                                                           >  1:1437992/1‑76 (MQ=255)
aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgc                                                                           >  1:3660717/1‑76 (MQ=255)
aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgc                                                                           <  2:1622717/76‑1 (MQ=255)
aGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgc                                                                           <  1:1939253/76‑1 (MQ=255)
aGATGGCACCTTACTTTCTCCCGACCATACGTTATCCCCTTACTCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgc                                                                           <  1:4627371/76‑1 (MQ=255)
 gATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgcg                                                                          >  2:2117724/1‑75 (MQ=255)
  aTGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgcgg                                                                         >  1:3125877/1‑74 (MQ=255)
   tGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgcggc                                                                        <  1:3607904/76‑4 (MQ=255)
   tGGCACGTTACTTTCTCCCGACCATACATTATCCCCTTACGCCAAACAAACTCTGAAGCTGCTCAC‑‑Cg‑‑cgcgcggc                                                                        >  2:3704895/1‑73 (MQ=255)
          ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAGAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACt                                                                 >  1:3374613/1‑76 (MQ=255)
          ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CGCACGCGG‑‑CATCAACt                                                                 >  2:456385/1‑76 (MQ=255)
          ttACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CC‑‑CGCGCGGCATCAACt                                                                 >  2:375272/1‑76 (MQ=255)
            aCTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACttt                                                               <  2:2620120/76‑1 (MQ=255)
            aCTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACttt                                                               <  2:814327/76‑1 (MQ=255)
              tttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTtgt                                                             >  1:3766643/1‑76 (MQ=255)
               ttCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTtgtg                                                            <  1:3584855/76‑1 (MQ=255)
                tctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTgtgt                                                           <  2:4069539/76‑1 (MQ=255)
                tcccccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTgtgt                                                           <  2:1150952/73‑1 (MQ=255)
                gcgcccGACCATACGGTATCCCCTGACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTgtgt                                                           <  2:1168994/73‑1 (MQ=255)
                 ctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGtt                                                          <  1:1659262/76‑1 (MQ=255)
                 ctcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGtt                                                          >  2:2931176/1‑76 (MQ=255)
                  tcCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGttt                                                         <  1:4690310/76‑1 (MQ=255)
                    ccGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGc                                                       <  1:1992816/76‑1 (MQ=255)
                     cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCg                                                      <  2:1182438/76‑1 (MQ=255)
                     cGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCg                                                      <  2:2960131/76‑1 (MQ=255)
                      gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGa                                                     >  2:658234/1‑76 (MQ=255)
                      gACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGa                                                     >  2:3739476/1‑76 (MQ=255)
                      gACCATAAGGTATCCCCTTACGCCATAGAAACTCTGAAACTGCTCAC‑‑CT‑‑CGCGCGGCATCAGCTTGGTGTTTGCgc                                                     >  1:4102297/1‑75 (MQ=255)
                       aCCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGAc                                                    >  1:743673/1‑76 (MQ=255)
                       aCCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGAc                                                    <  1:2713900/76‑1 (MQ=255)
                        ccATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGAcc                                                   >  1:3342783/1‑76 (MQ=255)
                         cATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCg                                                  <  1:2125355/76‑1 (MQ=255)
                          aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                 >  1:1424556/1‑76 (MQ=255)
                          aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                 >  2:2381699/1‑76 (MQ=255)
                          aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                 >  1:1712228/1‑76 (MQ=255)
                          aTACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCgg                                                 >  2:3723134/1‑76 (MQ=255)
                           tACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGgt                                                >  1:2691087/1‑76 (MQ=255)
                             cGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGgtcg                                              <  1:1736691/76‑1 (MQ=255)
                              gTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                             >  1:3952147/1‑76 (MQ=255)
                              gTTACCCCCTGACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgt                                             <  1:1100768/76‑1 (MQ=255)
                               ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCCACTTTGTTTTTGCGACCGgtcgcc                                            >  2:1474863/1‑74 (MQ=255)
                               ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                            <  1:3551885/76‑1 (MQ=255)
                               ttATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGgtcgtc                                            <  1:4545853/76‑1 (MQ=255)
                                 aTCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCACAGCG‑‑CGCGG‑‑CATCAACTGTGTCTTTGCGACCGGTCGTcac                                          >  2:2834862/1‑76 (MQ=255)
                                   ccccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcacca                                        <  1:3969005/76‑1 (MQ=255)
                                    cccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcaccac                                       <  2:3519224/76‑1 (MQ=255)
                                    cccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTcaccac                                       <  2:3132405/76‑1 (MQ=255)
                                     ccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg                                      <  2:281930/76‑1 (MQ=255)
                                     ccTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACg                                      >  1:4717083/1‑76 (MQ=255)
                                      cTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGt                                     <  2:617170/76‑1 (MQ=255)
                                       ttACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGtt                                    >  1:1152995/1‑76 (MQ=255)
                                        tACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTg                                   >  1:1248753/1‑76 (MQ=255)
                                        tACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTg                                   >  1:3426579/1‑76 (MQ=255)
                                        tACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGGTg                                   >  1:1821647/1‑76 (MQ=255)
                                         aCGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCCCCACGTTgt                                  >  2:152810/1‑75 (MQ=255)
                                          cGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                 >  2:3767062/1‑76 (MQ=255)
                                          cGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                 >  1:3147375/1‑76 (MQ=255)
                                          cGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                 <  1:4196276/76‑1 (MQ=255)
                                           gcaaaaGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGCCACCACGCTGAtg                                >  2:1154706/4‑76 (MQ=255)
                                           gCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtg                                >  2:2550941/1‑76 (MQ=255)
                                            ccAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgt                               <  1:748732/76‑1 (MQ=255)
                                             cAAAGAAGCTCTGTGCCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                              <  1:2742080/76‑1 (MQ=255)
                                             cAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                              >  1:874870/1‑76 (MQ=255)
                                             cAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAtgtg                              <  2:4528137/76‑1 (MQ=255)
                                              aaaGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTgg                             <  2:3715197/76‑1 (MQ=255)
                                               aaGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggg                            <  1:1303707/76‑1 (MQ=255)
                                               aaGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGCCGTCCCCCCGTTGCTGTggg                            >  2:262651/1‑76 (MQ=255)
                                                aGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTggag                           >  1:3800306/1‑74 (MQ=255)
                                                 gAAACGCTGAGGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGc                          <  1:4396000/76‑1 (MQ=255)
                                                  aaaCTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCa                         <  1:2494068/76‑1 (MQ=255)
                                                   aaCTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaa                        <  1:2160605/76‑1 (MQ=255)
                                                   aaCTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaa                        <  1:3862409/76‑1 (MQ=255)
                                                   aaCTCTGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCaa                        >  2:2501428/1‑76 (MQ=255)
                                                      tctGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                 <  1:3892071/64‑1 (MQ=255)
                                                      tctGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGAt                                 >  2:3892071/1‑64 (MQ=255)
                                                       ctGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc                    <  2:3585821/76‑1 (MQ=255)
                                                       ctGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc                    >  2:2202521/1‑76 (MQ=255)
                                                       ctGAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTc                    >  2:2542831/1‑76 (MQ=255)
                                                         gAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc                  >  2:1471102/1‑76 (MQ=255)
                                                         gAAGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgc                  <  1:1474864/76‑1 (MQ=255)
                                                          aaGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTcgcg                 >  1:2431036/1‑76 (MQ=255)
                                                           aGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGa                >  2:1057810/1‑76 (MQ=255)
                                                           aGCTGCTCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGa                >  1:2097234/1‑76 (MQ=255)
                                                            gctgctCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGAt               >  1:1316539/1‑76 (MQ=255)
                                                             ctgctCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATa              <  1:1821474/76‑1 (MQ=255)
                                                              tgctCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATaa             >  2:3378824/1‑76 (MQ=255)
                                                               gctCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt            >  2:4526100/1‑76 (MQ=255)
                                                               gctCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt            >  2:1861429/1‑76 (MQ=255)
                                                               gctCAC‑‑CG‑‑CGCGCGGCATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAAt            >  1:2005235/1‑76 (MQ=255)
                                                                    ccgcg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGga       <  1:513240/73‑1 (MQ=255)
                                                                    ccgcg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGAGGGGCAAATTCGCGATAATCTGga       >  1:4520915/4‑76 (MQ=255)
                                                                     cgcg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgag      <  2:4115760/74‑1 (MQ=255)
                                                                      gcg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga     >  2:2976560/2‑76 (MQ=255)
                                                                      gcg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga     >  1:1529006/2‑76 (MQ=255)
                                                                      gcg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga     >  1:4535753/2‑76 (MQ=255)
                                                                      gcg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGgaga     >  1:4446666/2‑76 (MQ=255)
                                                                       cg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt    <  1:890489/76‑1 (MQ=255)
                                                                       cg‑‑cgcgG‑‑CATCAACTTTGTGTTTGCGACCGGTCGGCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGAt    <  2:1658722/76‑1 (MQ=255)
                                                                           cgcgg‑‑CATCACCTTTGTGTTTGCGCCCGGTCGTCACCCCGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa  >  2:1791324/1‑76 (MQ=255)
                                                                           cgcgg‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTa  >  2:1755321/1‑76 (MQ=255)
                                                                                ||                                                                       
AGATGGCACGTTACTTTCTCCCGACCATACGTTATCCCCTTACGCCAAAGAAACTCTGAAGCTGCTCAC‑‑CG‑‑CGCGC‑‑CATCAACTTTGTGTTTGCGACCGGTCGTCACCACGTTGATGTGGGGCAAATTCGCGATAATCTGGAGATTA  >  NC_000913/4007832‑4007978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑