| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,008,469 | +G | intergenic (+36/‑197) | b4555 → / → yigM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,008,466 | 1 | . | G | 98.4% | 162.2 / ‑5.0 | 64 | intergenic (+33/‑200) | b4555/yigM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base G (27/35); minor base T (0/1); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTG > NC_000913/4008410‑4008534 | ccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCAtt < 2:2041517/75‑1 (MQ=255)ccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCAtt > 1:1830542/2‑76 (MQ=255) cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTa < 1:3575133/76‑1 (MQ=255) cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTa > 1:3924921/1‑76 (MQ=255) cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTa < 1:4411708/76‑1 (MQ=255) tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTAt > 1:1965006/1‑76 (MQ=255) gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATg > 1:2470019/1‑76 (MQ=255) aaGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATgg < 2:2711712/76‑1 (MQ=255) aaGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATgg < 2:1089164/76‑1 (MQ=255) tgtattgCGTTGGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTg < 2:2035636/73‑1 (MQ=255) ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCcgcg > 2:3397167/4‑76 (MQ=255) tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCgc > 2:1147751/3‑75 (MQ=255) tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGt < 2:1434903/74‑1 (MQ=255) tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGt < 2:2873027/74‑1 (MQ=255) gtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt < 1:2379453/76‑1 (MQ=255) gACGGGATGAACGACGCCGAAATGCTGTCGACGGC‑GGCGAAAGGCTGCATTATAGGCAGCGCACACCAGCGTCtca > 1:3894669/1‑74 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTgca > 1:3810150/1‑74 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGaa > 2:3618591/1‑76 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGaa < 2:3435984/76‑1 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAg > 1:2512601/1‑76 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAg < 1:4318325/76‑1 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGCGCGCACCACCGTCTGaaac > 2:898294/1‑75 (MQ=255) gggtGAACGACGCGGAAATGCTGTGGGGGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGa < 2:4739062/73‑1 (MQ=255) ggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGa < 2:1680721/76‑1 (MQ=255) gATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAc < 1:4349596/76‑1 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCCGTGCGCACCAGCGTCTGCAACAcc > 2:1443325/1‑76 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAcc > 2:3123186/1‑76 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAcc > 2:4304880/1‑76 (MQ=255) tGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCt < 1:3361916/76‑1 (MQ=255) tGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCt < 2:3339859/76‑1 (MQ=255) gAACGACGCCGAAATGCTGTCGATGACGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCtt < 1:2092071/76‑1 (MQ=255) tgcgacgCCGTGATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCATTTGGCGCCAGCTTCTGAAAGACCTTc < 2:3167314/74‑1 (MQ=255) gacgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc > 2:554602/1‑76 (MQ=255) gacgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGGGCGCACCAGCGTATGAAAGACCTTCCTc > 2:1316775/1‑76 (MQ=255) gacgCCGAAATGCGGGCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc < 2:3667113/76‑1 (MQ=255) acgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc > 2:2990825/1‑76 (MQ=255) acgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc < 2:3045707/76‑1 (MQ=255) cgtcGAAATGCGGCCGTTGGGTGG‑GAAATGCTGTCTTATGGGCCGTGCGCACCAGCGTCTGACCGACCTTCATccc < 2:1932311/73‑1 (MQ=255) cgCCGAGATGCTGGCGAGGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACGTTCATccc < 2:972160/76‑1 (MQ=255) cgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc > 2:2733047/1‑76 (MQ=255) cgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc < 2:2481421/76‑1 (MQ=255) gCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCCGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg > 1:3054200/1‑76 (MQ=255) gCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg > 1:1076628/1‑76 (MQ=255) ccGAGATGCTGGCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa < 2:265884/76‑1 (MQ=255) ccGAATTGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGTCCTTCCTCCCGa < 1:1443252/76‑1 (MQ=255) ccGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa < 1:3326156/76‑1 (MQ=255) ccGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa > 2:2528308/1‑76 (MQ=255) cGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg > 1:4465115/1‑76 (MQ=255) cGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg > 1:4074132/1‑76 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc < 1:109247/76‑1 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc > 1:1466152/1‑76 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc < 2:3924921/76‑1 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc < 2:2818113/76‑1 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGCGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc > 2:3773430/1‑76 (MQ=255) acaTGCTGTCGAGGGCGGG‑GAAAGGCTGCATTATGTGCAGTGCGCGCCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:2584880/74‑1 (MQ=255) aaaTGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:3760596/76‑1 (MQ=255) aTGCTGGCGATGGCGGG‑GAAAGGCTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg < 2:3448801/76‑2 (MQ=255) tGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga > 1:730843/1‑74 (MQ=255) gCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa < 1:2076657/76‑4 (MQ=255) gTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg > 2:3016000/1‑76 (MQ=255) cGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt < 1:2434551/76‑1 (MQ=255) cGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt < 2:3796450/76‑1 (MQ=255) cGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt < 2:3835462/76‑1 (MQ=255) aTGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg > 1:3514535/1‑76 (MQ=255) | GCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTG > NC_000913/4008410‑4008534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |