| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,008,525 | +G | intergenic (+92/‑141) | b4555 → / → yigM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,008,524 | 1 | . | G | 99.1% | 313.1 / ‑3.5 | 116 | intergenic (+91/‑142) | b4555/yigM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (48/67); total (48/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
AAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT > NC_000913/4008451‑4008594 | acaTGCTGTCGAGGGCGGGGAAAGGCTGCATTATGTGCAGTGCGCGCCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:2584880/74‑1 (MQ=255)aaaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:3760596/76‑1 (MQ=255) aTGCTGGCGATGGCGGGGAAAGGCTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg < 2:3448801/76‑2 (MQ=255) tGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga > 1:730843/1‑74 (MQ=255) gCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa < 1:2076657/76‑4 (MQ=255) gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTg > 2:3016000/1‑76 (MQ=255) cGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt < 2:3796450/76‑1 (MQ=255) cGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt < 2:3835462/76‑1 (MQ=255) cGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt < 1:2434551/76‑1 (MQ=255) aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTg > 1:3514535/1‑76 (MQ=255) gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTa < 2:538722/73‑1 (MQ=255) cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTAc < 2:3054200/74‑1 (MQ=255) ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACt > 1:594061/2‑76 (MQ=255) gggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑CAGTGATTGGTACTa > 2:1580058/1‑76 (MQ=255) gggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTa > 1:3900375/1‑76 (MQ=255) ggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAGCTTCATCCCGAGCTGG‑AAGTGATTGGTACTaa < 2:4094724/76‑1 (MQ=255) gAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATg < 2:1146351/76‑1 (MQ=255) aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGc > 2:2895791/4‑76 (MQ=255) aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGc > 2:737636/4‑76 (MQ=255) aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGcc < 1:4548023/74‑1 (MQ=255) ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg > 1:1435341/2‑76 (MQ=255) ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg < 1:1211114/75‑1 (MQ=255) gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcga < 1:1967565/76‑1 (MQ=255) gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcga < 1:673755/76‑1 (MQ=255) tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacg > 2:4000117/1‑76 (MQ=255) tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacg > 2:4152501/1‑76 (MQ=255) gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑GAGTGATTGGTACTAATGCcgacga > 2:1422163/1‑76 (MQ=255) gCATTAGGGGCAGGGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacga < 2:150081/76‑1 (MQ=255) cATTATGGGCAGTGCGCGCCAGCGTCTGAAAGACCTGCATCCCGAGCT‑GTAAGTGATTTGTACTAATGCCgacgac < 1:3405968/76‑1 (MQ=255) cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGATGTGG‑AAGTGATTGGTACTAATGCCgacgac < 1:2559158/76‑1 (MQ=255) cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac < 1:2516127/76‑1 (MQ=255) cATTATGGGCAGTGCGCACCAGCGTCTGAAAGAACTTCATCCCGAGCTGG‑ACGGGATTGGTACTAATGCCgatgac > 2:3241215/1‑76 (MQ=255) cATTAGGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac < 2:3646296/76‑1 (MQ=255) aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGacgacg < 1:2410777/76‑1 (MQ=255) ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgc < 1:3542838/76‑1 (MQ=255) tgaTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgc < 1:511002/74‑1 (MQ=255) tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg < 2:4465115/76‑1 (MQ=255) tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg < 1:2892318/76‑1 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGCCCACGCgg > 2:4799969/1‑76 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCgg > 2:3589337/1‑76 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCgg > 2:3584729/1‑76 (MQ=255) tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt < 1:4103645/76‑1 (MQ=255) tGGGCAGGGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt < 2:582257/76‑1 (MQ=255) gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTg < 2:2545943/76‑1 (MQ=255) gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTg < 1:2213306/76‑1 (MQ=255) ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGc < 1:898295/76‑1 (MQ=255) gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGcc < 2:3238767/76‑1 (MQ=255) gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt > 2:2356529/4‑76 (MQ=255) gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt < 2:1732972/73‑1 (MQ=255) cgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa < 2:3097448/74‑1 (MQ=255) caccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGTTATTAATGCAGACGCACCGGTGTCGtattatt > 1:3983709/1‑75 (MQ=255) caccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc < 2:2848143/76‑1 (MQ=255) accaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt > 2:2134340/1‑76 (MQ=255) accaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt > 2:1001007/1‑76 (MQ=255) ccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg < 1:4864459/76‑1 (MQ=255) caGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAGGCGGTGCCGCATTACCTGc < 2:1822726/76‑1 (MQ=255) caGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc < 1:4335048/76‑1 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg < 1:2019843/76‑1 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg < 2:1441815/76‑1 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg > 2:287322/1‑76 (MQ=255) aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg < 1:4075634/76‑1 (MQ=255) gCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt < 1:4793629/76‑1 (MQ=255) cGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAACTGCGTa > 1:3678532/1‑76 (MQ=255) cGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGATCCTAATGCCGCCGACGCGGTGCCGCATTATCTGCGTa < 1:4512455/76‑1 (MQ=255) gTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa < 2:1533823/76‑1 (MQ=255) gTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa > 2:417211/1‑76 (MQ=255) tCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa < 1:3764795/76‑1 (MQ=255) cTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc < 2:3852194/76‑1 (MQ=255) cTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc > 2:1159331/1‑76 (MQ=255) cTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc > 1:3484655/1‑76 (MQ=255) tGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg > 2:4023065/1‑68 (MQ=255) tGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg < 1:4023065/68‑1 (MQ=255) tGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct < 1:598939/76‑1 (MQ=255) gAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActc < 2:2153935/76‑1 (MQ=255) gAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActc > 2:1227101/1‑76 (MQ=255) gAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActc > 1:4430839/1‑76 (MQ=255) aaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCCTTATCTGCGTAAActctt > 2:31234/1‑75 (MQ=255) aaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa < 1:71536/76‑1 (MQ=255) aaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa < 1:417211/76‑1 (MQ=255) aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacga > 1:1176460/1‑45 (MQ=255) aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacga < 2:1176459/45‑1 (MQ=255) aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTACACTCTAt > 2:792859/1‑76 (MQ=255) aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAt < 1:1955800/76‑1 (MQ=255) aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGCGCCGCATTATCTGCGTAAACTCTAt > 2:9189/1‑76 (MQ=255) gACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt > 1:3798502/1‑76 (MQ=255) gcTTCGTCCCGAGGTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa < 2:88020/75‑1 (MQ=255) cTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACTCGGTGCCGCATTATCTGCGTAAACTCTATTTAt < 2:3181370/76‑1 (MQ=255) cTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt < 2:2019076/76‑1 (MQ=255) ttCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc < 2:3779939/76‑1 (MQ=255) tCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg < 2:4375132/76‑1 (MQ=255) cATCCCGAGCTGG‑AAGTGATTGGGACTAATGCCGACGACGAGGTGCCGCATTATCTTCGTAAACGCTATTTATAGt > 1:4623292/1‑76 (MQ=255) cccGAGCTGG‑GAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt < 1:1422164/76‑1 (MQ=255) cGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg > 1:3100030/1‑76 (MQ=255) cGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg > 1:3096441/1‑76 (MQ=255) cGAGCTGG‑AAGTGATTGGGACGAATGCCGACGACTCTGTGCCGCATTATCTGCGTAAACTCTATTTATCGTaagcg > 1:3100405/1‑73 (MQ=255) cGAGCTGG‑AAGAGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATATGCGTAAACTCTATTTATCGTAATCg > 1:1163193/1‑76 (MQ=255) gAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt > 1:4806687/1‑76 (MQ=255) gAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt < 1:307316/76‑1 (MQ=255) gAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt > 2:2531386/1‑76 (MQ=255) gAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt > 2:3430864/1‑76 (MQ=255) | AAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT > NC_000913/4008451‑4008594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |