| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,057,759 | +C | coding (1773/1776 nt) | yihK → | putative GTP‑binding factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,057,758 | 1 | . | C | 99.1% | 282.8 / ‑4.2 | 110 | coding (1772/1776 nt) | yihK | putative GTP‑binding factor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (32/77); total (32/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA > NC_000913/4057683‑4057828 | aaCAAGCTTTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCa < 1:2637811/76‑1 (MQ=255) cAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cc > 1:1410010/1‑75 (MQ=255) cAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cc > 1:4863282/1‑75 (MQ=255) aGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cctg < 2:2528606/76‑4 (MQ=255) aGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cctg > 1:1804445/1‑73 (MQ=255) aGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑cctg < 2:4633916/76‑4 (MQ=255) tctGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACg > 2:3060203/1‑76 (MQ=255) tctGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACg > 2:1136731/1‑76 (MQ=255) tGGGGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGa < 2:557234/76‑1 (MQ=255) tGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGa > 2:42609/1‑76 (MQ=255) ggAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaa > 1:1555194/1‑76 (MQ=255) gAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaaa < 1:3863564/76‑1 (MQ=255) gAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaaa < 1:4154972/76‑1 (MQ=255) gAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGaaa < 1:4611346/76‑1 (MQ=255) gTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAAc < 1:2567454/76‑1 (MQ=255) ttCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACg < 2:33668/76‑1 (MQ=255) tCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGa < 2:3675437/76‑1 (MQ=255) tCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGa < 2:1483680/76‑1 (MQ=255) cATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAACCGAt > 2:3489294/1‑76 (MQ=255) ctCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtc < 2:3642464/75‑1 (MQ=255) aTCGATGATGACGAACTGGTAGAAGTGACTCAGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtc < 2:1066740/76‑1 (MQ=255) aTCGATGAGGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtc < 2:4453705/76‑1 (MQ=255) cGATGGTGACGAACTGGGAGAAGTGACTCCGGCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgt < 2:4780169/76‑1 (MQ=255) cGATGATGACGAACTGGTAGAAGCGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgc > 1:233950/1‑75 (MQ=255) gatgctGCCGGACTGGTAGAAGTGGCTCCGGCCTCTATCCGTCTTGGTGAACGTCACCTGACGGAAAACGAtcttc < 2:1245299/76‑1 (MQ=255) gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgtc > 2:511199/1‑76 (MQ=255) gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgtc > 1:3936796/1‑76 (MQ=255) gatgatGACGAACTGGGAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgtc < 2:295558/76‑1 (MQ=255) gtgatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgtcg < 2:684998/75‑1 (MQ=255) atgatgACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgtcg > 1:645269/1‑76 (MQ=255) atgatgACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGAtcgtcg > 1:4846948/1‑76 (MQ=255) tgatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgc < 1:4381806/76‑1 (MQ=255) tgatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgc < 2:3548417/76‑1 (MQ=255) tatgaCGAACTGGGAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACAGAAAACGATCGTcgcc < 2:464987/75‑1 (MQ=255) gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgcc < 1:781049/76‑1 (MQ=255) gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgcc < 1:3647306/76‑1 (MQ=255) gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgcc > 2:3484282/1‑76 (MQ=255) atgaCGAACTGGTAGAAGTGGCTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgccg < 2:178746/76‑1 (MQ=255) atgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgccg > 2:3350430/1‑76 (MQ=255) atgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgccg > 2:3937897/1‑76 (MQ=255) tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTcgccgc < 2:2695415/76‑1 (MQ=255) gaCGAGCTTGTGGTAGTGACTCCGACCTCTAGCTGTATTCTTAATCGCCGCCGGTCTGAAAACTATCGTCGCcgcg < 1:140288/76‑1 (MQ=255) cGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGcc > 1:2848523/1‑76 (MQ=255) gAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCa < 1:4859485/76‑1 (MQ=255) aCTGGTGAAAGTGACTCCGGCCTCTATCCGTGTTCGTAAACGTCACCTGACGGAAAACGATCTTCGCCGCGCCAAc < 2:4774543/76‑1 (MQ=255) aCTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAAc < 2:3544969/76‑1 (MQ=255) cTGGTAGAAGTGACTCCGCCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAAcc < 1:2433180/76‑1 (MQ=255) cTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGCAAACGATCGGCCCCGGGCCAAcc > 1:388436/1‑76 (MQ=255) cTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAAcc < 1:4136997/76‑1 (MQ=255) tGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACcg < 1:3144946/76‑1 (MQ=255) tGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACcg < 2:4013274/76‑1 (MQ=255) ggTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACcga > 1:4784429/1‑75 (MQ=255) gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACcgcg < 2:2597521/76‑1 (MQ=255) gTAGAAGTGACTCCGACCCCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACcgcg < 2:2609025/76‑1 (MQ=255) tAGAAGTGACTCCGCCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCgcgc < 2:70299/76‑1 (MQ=255) tAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCgcgc < 2:1854219/76‑1 (MQ=255) tAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCgcgc < 2:1975108/76‑1 (MQ=255) tAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCgcgc < 2:4760808/76‑1 (MQ=255) aGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCa < 1:2497945/76‑1 (MQ=255) gAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAc > 2:3336336/1‑76 (MQ=255) aaaGTGACTCCGCCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAc < 1:3027815/75‑1 (MQ=255) aaGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAcc < 2:4283962/76‑1 (MQ=255) aaGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAcc > 1:18426/1‑76 (MQ=255) aGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCg < 1:3146068/76‑1 (MQ=255) aGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCg < 2:3126109/76‑1 (MQ=255) gACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGaaa < 1:4310556/76‑1 (MQ=255) aCTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCTCCAACCGCGCACCGAAAg < 1:1495106/76‑1 (MQ=255) aCTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAg < 1:3685474/76‑1 (MQ=255) cTCCTAACTCTATCCGTAGTCGTAAACGGCACCTAACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGa < 1:2787824/76‑1 (MQ=255) cTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGa > 1:3186757/1‑76 (MQ=255) tCCGCCCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAc < 1:1073736/76‑1 (MQ=255) ccGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACg > 2:3491527/1‑76 (MQ=255) ccGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACg < 2:4863282/76‑1 (MQ=255) cGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGACAGACGa > 1:3779493/1‑76 (MQ=255) cGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGa < 1:3217625/76‑1 (MQ=255) cGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGa > 1:3042433/1‑76 (MQ=255) cGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGa < 1:1865634/76‑1 (MQ=255) cGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGa > 1:3877277/1‑76 (MQ=255) gACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAt < 2:1518469/76‑1 (MQ=255) gACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAt > 2:3771564/1‑76 (MQ=255) gccTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAtt < 2:2159143/75‑1 (MQ=255) ccTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTa > 2:3284988/1‑76 (MQ=255) ccTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTa < 1:2834215/76‑1 (MQ=255) ccTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTa < 1:2392675/76‑1 (MQ=255) ctctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTaa < 2:540712/76‑1 (MQ=255) aTCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTc < 1:1723032/76‑1 (MQ=255) tCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCg < 1:41037/76‑1 (MQ=255) gcGTATTGGTAAACGTGACCTGACGGAAACCGATCGTCGCCGCGCCAACCGCGCACCGAAAGGCGATTAATTTCGt < 2:2114639/75‑1 (MQ=255) ccGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGt > 1:2119944/1‑76 (MQ=255) gTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGttt < 1:2671858/76‑1 (MQ=255) tATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGtttt < 1:1368419/76‑1 (MQ=255) tATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGtttt < 2:2008964/76‑1 (MQ=255) ttCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGCCGATTAATTTCGtttttt > 2:135405/1‑76 (MQ=255) tCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTa < 2:1486141/76‑1 (MQ=255) cGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAg < 1:2656979/76‑1 (MQ=255) tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTc < 2:2956436/76‑1 (MQ=255) tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTc < 2:3795562/76‑1 (MQ=255) tAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTc < 1:2664515/76‑1 (MQ=255) aaaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCa < 1:2253309/76‑1 (MQ=255) aaCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAt > 1:1535173/1‑76 (MQ=255) | AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA > NC_000913/4057683‑4057828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |