| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,175,949 | +G | intergenic (+82/‑76) | nusG → / → rplK | NusG/50S ribosomal subunit protein L11 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,175,947 | 1 | . | G | 98.5% | 168.7 / ‑3.2 | 66 | intergenic (+80/‑78) | nusG/rplK | NusG/50S ribosomal subunit protein L11 |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (34/31); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGA > NC_000913/4175872‑4176017 | gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑At < 1:4117363/76‑1 (MQ=255) cGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑g > 2:1759301/1‑76 (MQ=255) gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑gg < 1:3195618/76‑1 (MQ=255) gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑gg < 1:961097/76‑1 (MQ=255) gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑gg < 2:3738742/76‑1 (MQ=255) gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑gg < 1:3488674/76‑1 (MQ=255) aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑ggg > 2:1288520/1‑75 (MQ=255) tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑gggc > 1:3037578/1‑74 (MQ=255) tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑gggc > 1:3150507/1‑74 (MQ=255) tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGACTTGTGTTTTT‑AT‑ggga > 1:3134479/1‑74 (MQ=255) aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGcc > 1:4344420/1‑76 (MQ=255) aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGcc > 1:3358695/1‑76 (MQ=255) aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGccc < 1:2713941/76‑1 (MQ=255) aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGccc < 1:2756487/76‑1 (MQ=255) gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCg < 2:3779551/76‑1 (MQ=255) gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCg < 1:332433/76‑1 (MQ=255) gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTaa > 1:145767/1‑76 (MQ=255) cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTaaa < 2:1880909/76‑1 (MQ=255) aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTaaa < 2:2814297/74‑1 (MQ=255) aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTaaa > 1:2814297/1‑74 (MQ=255) aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAAc > 1:3172227/1‑76 (MQ=255) tAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAt > 1:1654440/1‑76 (MQ=255) aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAttt > 1:1755757/1‑76 (MQ=255) aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAttt > 1:66848/1‑76 (MQ=255) aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAttt < 2:1632845/76‑1 (MQ=255) tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAtttt < 2:3590367/76‑1 (MQ=255) cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAttttt > 1:1457915/1‑76 (MQ=255) cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACACttttt > 2:322209/1‑76 (MQ=255) ggtGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAtttttt < 2:1597647/74‑1 (MQ=255) gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAGCGAtttttt > 1:4383054/1‑76 (MQ=255) gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGAtttttt > 2:848037/1‑76 (MQ=255) tGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTtat < 1:5149/76‑1 (MQ=255) gCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTtata < 2:4446047/76‑1 (MQ=255) gCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTtata > 1:3107499/1‑76 (MQ=255) acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATc > 2:420578/1‑76 (MQ=255) acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATc > 1:4435393/1‑76 (MQ=255) caAGGCGTGAGATTGGAATACAATTTCGCGCCTGTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCa < 1:2462780/76‑1 (MQ=255) aaGGCTTGAGATAGAAATACAATTTCGCGCCCTCTGTTTTTCTT‑GGCCTTGCCCCCCAAACTATTTTTTATATccc < 1:4730652/76‑3 (MQ=255) aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCAc < 2:4461716/76‑1 (MQ=255) ggCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg > 2:1794327/1‑76 (MQ=255) gCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACggg < 1:386743/76‑1 (MQ=255) gCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACggg > 1:1565985/1‑76 (MQ=255) cGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgggg > 1:3636847/1‑76 (MQ=255) cGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgggg > 1:2697072/1‑76 (MQ=255) gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGa < 1:1288521/76‑1 (MQ=255) tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAg > 2:3516994/1‑76 (MQ=255) tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAg < 2:3158605/76‑1 (MQ=255) gagaTTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc > 1:3106503/1‑76 (MQ=255) agaTTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc < 2:145767/76‑1 (MQ=255) agaTTGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc < 2:977033/76‑1 (MQ=255) ttGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct < 2:4455854/76‑1 (MQ=255) tGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc < 2:4435393/76‑1 (MQ=255) tGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc < 1:246532/76‑1 (MQ=255) tGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc < 2:2739910/76‑1 (MQ=255) tGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGCGAGCCtctc > 2:2118207/1‑76 (MQ=255) tGGAATACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATAACGGGGAGCCtctc > 1:131138/1‑76 (MQ=255) aaTACAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCaga < 2:2923065/76‑1 (MQ=255) aCAATTTCGCGGCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc < 2:4554139/76‑1 (MQ=255) aCAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc > 1:3760259/1‑76 (MQ=255) aCAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc < 1:2110653/76‑1 (MQ=255) aCAATTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc < 2:2307804/76‑1 (MQ=255) aTTTCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtt > 1:3322618/1‑76 (MQ=255) tCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt < 1:4650828/76‑1 (MQ=255) tCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt < 2:3772500/76‑1 (MQ=255) tCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt < 1:4739755/76‑1 (MQ=255) tCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt < 1:3753311/76‑1 (MQ=255) tCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt > 2:4501172/1‑76 (MQ=255) tCGCGCCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGgtatt > 1:3668392/1‑76 (MQ=255) gcgcCTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAc > 1:4293546/1‑76 (MQ=255) gcCTTTTGTTTTT‑AGGGGCCTTGCCCGAAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATGAccc > 1:4593304/1‑76 (MQ=255) ccTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa > 1:4714505/1‑76 (MQ=255) ccTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa > 2:4731467/1‑76 (MQ=255) ccTTTTGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa > 1:1355519/1‑76 (MQ=255) ttttGTTTTT‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc > 2:1721722/1‑76 (MQ=255) ttGTTTTT‑TTGGGGCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACtt < 2:303044/76‑1 (MQ=255) ttt‑ATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCCACTTGAGGa > 1:1769571/1‑76 (MQ=255) | GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT‑AT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGA > NC_000913/4175872‑4176017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |