BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,311,496	+C	coding (301/408 nt)	phnQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,311,495	1	.	C	100.0%	290.3 / NA	107	V101G (GTG→GGG)	phnQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (64/43); total (64/43)

Only 100 of 111 total aligned reads displayed.

GATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑CGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTAT  >  NC_000913/4311420‑4311565

gATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCa                                                                         >  2:794227/1‑76 (MQ=255)

  tAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑cc                                                                      <  2:1772142/76‑2 (MQ=255)

  gagagGTTCTTGTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑cc                                                                      <  2:362094/75‑2 (MQ=255)

    gagTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑ccgt                                                                    >  1:586240/1‑73 (MQ=255)

       tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTcc                                                                  <  2:1234732/76‑1 (MQ=255)

       tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTcc                                                                  >  1:3570515/1‑76 (MQ=255)

       tttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTcc                                                                  <  1:831836/76‑1 (MQ=255)

        ttCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCg                                                                 <  1:3293530/76‑1 (MQ=255)

        ttCTTTGTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCGCCGTTCCg                                                                 <  1:3882086/76‑1 (MQ=255)

          cTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGc                                                               >  1:4322149/1‑76 (MQ=255)

          cTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGc                                                               <  2:2499963/76‑1 (MQ=255)

          cTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGc                                                               <  2:4235925/76‑1 (MQ=255)

            ttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGccc                                                             >  2:3582873/1‑76 (MQ=255)

            ttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGccc                                                             >  1:3372365/1‑76 (MQ=255)

            ttttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGccc                                                             >  1:2786428/1‑76 (MQ=255)

             tttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCa                                                            <  1:3863265/76‑1 (MQ=255)

             tttGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGCTCCCGCCCa                                                            >  2:725174/1‑76 (MQ=255)

               tGAGGTTGGGTGATGAGACGCTTTCCGCTATTTCTTTTATTCGCCCTGCTCACGCTGTCCACCGTTCCGGCCCAgg                                                          <  2:3830201/76‑1 (MQ=255)

               tGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAgg                                                          <  1:3582873/76‑1 (MQ=255)

                gAGGTTGGGTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCatgc                                                         <  1:4693351/76‑4 (MQ=255)

                gAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCCGGc                                                         >  2:4459781/1‑76 (MQ=255)

                gAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCCCACGCTCTCCACCGTTCCGGACCAGGc                                                         >  2:1920297/1‑76 (MQ=255)

                    ttGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGat                                                     <  1:4797121/76‑1 (MQ=255)

                     tGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGata                                                    <  2:2569691/76‑1 (MQ=255)

                      ggCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat                                                   >  1:651902/1‑76 (MQ=255)

                      ggCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGatat                                                   >  2:2516608/1‑76 (MQ=255)

                       gCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatt                                                  <  1:2240319/76‑1 (MQ=255)

                       gCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatt                                                  <  1:1947921/76‑1 (MQ=255)

                        cTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatta                                                 <  1:1430197/76‑1 (MQ=255)

                        cTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatta                                                 >  1:2409297/1‑76 (MQ=255)

                         tAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtattat                                                <  1:1044733/76‑1 (MQ=255)

                           aTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCCCCGTTCCGGCCCAGGCCGATATTATcg                                              >  2:1502140/1‑76 (MQ=255)

                           aTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcg                                              <  2:1088813/76‑1 (MQ=255)

                           aTGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcg                                              <  2:4663658/76‑1 (MQ=255)

                            tGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcga                                             >  2:1926322/1‑76 (MQ=255)

                             gAAACGCTTTCCGCTATTTCTTTTATTCCCCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac                                            <  2:2818268/76‑1 (MQ=255)

                             gAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac                                            >  2:639017/1‑76 (MQ=255)

                              aaaCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgacg                                           >  2:2315386/1‑76 (MQ=255)

                               aaCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgacga                                          >  1:3812515/1‑76 (MQ=255)

                                aCGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCgccgac                                         >  2:4757666/1‑76 (MQ=255)

                                 cGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGaca                                        <  1:2020616/76‑1 (MQ=255)

                                  gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGacac                                       >  1:3448672/1‑76 (MQ=255)

                                  gCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGacac                                       >  1:693436/1‑76 (MQ=255)

                                      tCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAtt                                   >  2:2898913/1‑76 (MQ=255)

                                      tCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCAtt                                   >  1:857782/1‑76 (MQ=255)

                                      tCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATACACCACACCAtt                                   >  1:2494902/1‑76 (MQ=255)

                                       ccGCTATTTCTTTTATTCCCCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTg                                  <  1:475017/76‑1 (MQ=255)

                                       ccGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTg                                  <  1:873333/76‑1 (MQ=255)

                                        cGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTgg                                 <  1:2071245/76‑1 (MQ=255)

                                          cTATTTCTTTTATTCGCCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCa                               <  2:3451588/76‑1 (MQ=255)

                                          cTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCa                               >  1:1597560/1‑76 (MQ=255)

                                           tATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                              >  1:3329181/1‑76 (MQ=255)

                                           tATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCaa                              >  2:4238961/1‑76 (MQ=255)

                                            aTTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAat                             >  2:344868/1‑76 (MQ=255)

                                             tttCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAata                            >  1:3673939/1‑76 (MQ=255)

                                              ttCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAatat                           >  1:3666115/1‑76 (MQ=255)

                                                cTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTc                         <  1:2278855/76‑1 (MQ=255)

                                                cTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTc                         >  2:4306046/1‑76 (MQ=255)

                                                 ttttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTca                        >  1:799881/1‑76 (MQ=255)

                                                 ttttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTca                        >  2:751272/1‑76 (MQ=255)

                                                 ttttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTca                        >  1:227368/1‑76 (MQ=255)

                                                 ttttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTca                        >  2:1696875/1‑76 (MQ=255)

                                                   ttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                      >  1:1441683/1‑76 (MQ=255)

                                                   ttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                      >  2:3275586/1‑76 (MQ=255)

                                                   ttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                      <  1:442606/76‑1 (MQ=255)

                                                   ttATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagc                      >  2:1354718/1‑76 (MQ=255)

                                                    tATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                     <  1:656333/76‑1 (MQ=255)

                                                    tATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                     >  1:3085561/1‑76 (MQ=255)

                                                    tATTCACCCTGCTAACGCTGTCCACCGTTCCGGCCCAGGCCGATATGATCGACGACACCATTGGCAATATTcagca                     >  1:1489172/1‑76 (MQ=255)

                                                    tATTCACCCTGCGCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                     <  2:4214160/76‑1 (MQ=255)

                                                    tATTCACCCGGCTCGCGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagca                     <  2:2251065/76‑1 (MQ=255)

                                                     aTTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTcagcag                    <  1:3045895/76‑1 (MQ=255)

                                                      ttCCCCCTGCTCTCGCTGTCCACCGTTCCGGCCCAGGGCGATATTATCGACGACCCCATTGGCAATATTCAGCAgg                   <  1:3927438/76‑1 (MQ=255)

                                                      ttCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAgg                   >  1:3960690/1‑76 (MQ=255)

                                                      ttCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAgg                   >  2:2575944/1‑76 (MQ=255)

                                                        cccccTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCg                 <  1:239722/74‑1 (MQ=255)

                                                        cACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATCTTCAGCAGGCg                 >  1:337287/1‑76 (MQ=255)

                                                        cACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCg                 >  1:2309708/1‑76 (MQ=255)

                                                         aCCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATCTTCAGCAGGCGa                >  2:4851681/1‑76 (MQ=255)

                                                         aCCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGa                >  2:4158630/1‑76 (MQ=255)

                                                          cccTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAt               >  2:1395777/1‑76 (MQ=255)

                                                            cTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCACTATTCAGCAGGCGATTa             >  2:259519/1‑76 (MQ=255)

                                                            cTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTa             >  1:572768/1‑76 (MQ=255)

                                                             tGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTaa            >  1:1479590/1‑76 (MQ=255)

                                                              gCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAAt           >  1:3986663/1‑76 (MQ=255)

                                                               cTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATg          <  2:2721777/76‑1 (MQ=255)

                                                               cTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATg          >  1:4320219/1‑76 (MQ=255)

                                                               cTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATg          <  1:1549319/76‑1 (MQ=255)

                                                                tCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGa         <  1:3717342/76‑1 (MQ=255)

                                                                tCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGa         >  2:1281229/1‑76 (MQ=255)

                                                                tCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGa         >  2:3129938/1‑76 (MQ=255)

                                                                ccccGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGa         <  2:585189/73‑1 (MQ=255)

                                                                 cACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAc        <  2:2930548/76‑1 (MQ=255)

                                                                  aCGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACg       >  2:3616732/1‑76 (MQ=255)

                                                                  aCGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACg       >  1:267343/1‑76 (MQ=255)

                                                                  aCGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACg       >  2:1407711/1‑76 (MQ=255)

                                                                    gCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt     >  1:3453747/1‑76 (MQ=255)

                                                                    gCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCt     >  1:3453871/1‑76 (MQ=255)

                                                                     cTGTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCtt    >  2:2815237/1‑76 (MQ=255)

                                                                       gTCCACCGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTtat  <  1:4648247/76‑1 (MQ=255)

GATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCA‑CGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTAT  >  NC_000913/4311420‑4311565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`