| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,448,543 | +C | intergenic (+48/‑90) | ytfQ → / → ytfR | putative LACI‑type transcriptional regulator/YtfR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,448,542 | 1 | . | C | 97.9% | 229.8 / ‑0.5 | 96 | intergenic (+47/‑91) | ytfQ/ytfR | putative LACI‑type transcriptional regulator/YtfR |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (62/32); total (63/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCG‑G‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGC > NC_000913/4448478‑4448610 | aaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCgg > 1:2187051/1‑76 (MQ=25) aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGc < 2:1255121/76‑1 (MQ=25) aaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCg > 2:987746/1‑76 (MQ=25) aaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCg > 1:1706126/1‑76 (MQ=25) aaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCg > 2:2503734/1‑76 (MQ=25) aaaGATGGGGTATTGGGGTGTGCTATGCCTGATGCCGATTCGTAGGCCGGTTAAGGCGCTCGCGCC‑GCATCCGGCg < 2:1612175/76‑1 (MQ=17) atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGcatt > 2:3415414/1‑73 (MQ=21) atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGAt > 1:1373035/1‑76 (MQ=25) atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGAt > 1:3215376/1‑76 (MQ=25) tatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATg < 2:2890995/76‑1 (MQ=25) atGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATgg > 2:3567460/1‑76 (MQ=25) atGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATgg > 2:3224235/1‑76 (MQ=25) atGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGACgg > 1:3516982/1‑76 (MQ=21) tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGt < 1:1179815/76‑1 (MQ=25) ggggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTg < 2:1465640/76‑1 (MQ=25) gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCCTCCGGCGATGGGGc > 2:238068/1‑76 (MQ=21) gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGc > 1:3740216/1‑76 (MQ=25) gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGc < 1:3695471/76‑1 (MQ=25) ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCa > 2:3921908/1‑76 (MQ=25) gTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCAc > 1:3845456/1‑76 (MQ=25) tATTGAGGGTTGCTATGCCTGCTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGTGGCACt > 2:1456540/1‑76 (MQ=18) tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACt > 1:2109300/1‑76 (MQ=25) tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACt > 2:1437884/1‑76 (MQ=25) tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACt > 2:2098347/1‑76 (MQ=25) aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCCTCCGGCGATGGTGCACTg > 2:1716693/1‑76 (MQ=21) aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTg > 1:3413047/1‑76 (MQ=25) aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTg > 1:701596/1‑76 (MQ=25) aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTg > 1:3051415/1‑76 (MQ=25) aTTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTg > 1:3025234/1‑76 (MQ=25) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGaa < 2:4124228/76‑1 (MQ=21) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGaa > 2:4122953/1‑76 (MQ=25) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGaa > 2:3018356/1‑76 (MQ=25) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGaa > 2:2721019/1‑76 (MQ=25) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCG‑CTGCATCCGGCGATGGTGCACTGaa > 2:2385072/1‑76 (MQ=25) gAGGGTTGCTATGCCTGATGCTGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAg > 2:1955528/1‑76 (MQ=21) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAg > 1:1472479/1‑76 (MQ=25) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAg < 2:2406757/76‑1 (MQ=21) ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCt > 1:145960/1‑76 (MQ=25) ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCt < 2:3762852/76‑1 (MQ=25) gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTg > 1:2226689/1‑76 (MQ=25) gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTg > 1:52655/1‑76 (MQ=25) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGa > 2:1575129/1‑76 (MQ=21) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGa > 2:3338077/1‑76 (MQ=25) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGa < 2:3516662/76‑1 (MQ=21) tGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGAt > 1:222234/1‑76 (MQ=25) gggaTGCCTGATGCCGGTTCGTGGGCCGGTTAAGGCGCTCGCGCC‑GCGTCCGGCGATGGTGCACTGAAGCCTGATg < 2:4349330/73‑1 (MQ=18) gCTGTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATg < 2:4567735/76‑1 (MQ=21) cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGCGGGGGCACTGCAGCCCGCTGc > 2:919307/1‑76 (MQ=9) cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc > 1:4668642/1‑76 (MQ=21) cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGc < 1:1906713/76‑1 (MQ=21) tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCg > 1:4854678/1‑76 (MQ=21) tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCg > 1:371384/1‑76 (MQ=21) aTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGTTGCGa < 1:1392294/76‑1 (MQ=21) cTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCt < 1:4004728/76‑1 (MQ=21) cTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCt > 2:1604754/1‑76 (MQ=21) tGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAg > 2:4416917/1‑61 (MQ=18) tGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAg < 1:4416917/61‑1 (MQ=18) gATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa < 1:416030/76‑1 (MQ=21) gATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa < 2:2688923/76‑1 (MQ=21) gATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa < 1:4464802/76‑1 (MQ=21) tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc > 2:4218502/1‑76 (MQ=21) tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc < 2:408277/76‑1 (MQ=21) tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc > 1:3279991/1‑76 (MQ=21) tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc > 1:1102401/1‑76 (MQ=21) tGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc < 2:4224790/76‑1 (MQ=21) gCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg < 1:3930208/76‑1 (MQ=21) gCCGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg > 1:2577619/1‑76 (MQ=21) ccGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGTGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc > 1:3243150/1‑76 (MQ=21) cGATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgcg > 1:3981010/1‑76 (MQ=21) gATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgcgc > 2:2526542/1‑75 (MQ=21) gATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt > 1:4090780/1‑76 (MQ=21) gATTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGt < 2:810724/76‑1 (MQ=21) aTTCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTc < 1:2769159/76‑1 (MQ=21) ttCGTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt > 2:1364117/1‑76 (MQ=21) ttCGTAGGCCGGATAAGGAGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTCCCGCGTCt > 2:1838914/1‑76 (MQ=18) gTAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAt > 2:3770963/1‑76 (MQ=21) tAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc > 1:2642467/1‑76 (MQ=21) tAGGCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATc > 1:2942965/1‑76 (MQ=21) ggCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt > 1:2980077/1‑76 (MQ=18) ggCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt > 1:4049408/1‑76 (MQ=18) gCCGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg > 1:3410944/1‑76 (MQ=18) ccGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc < 1:99975/76‑1 (MQ=21) ccGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc < 1:1370359/76‑1 (MQ=18) ccGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc < 2:3290030/76‑1 (MQ=21) cGGATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGAAGCTTACCGCGTCTGATCCTGcc > 2:3954980/1‑76 (MQ=18) ggATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCt > 2:2688720/1‑76 (MQ=21) gATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGACGCCTGATGCGACGCTTACCGCGTCTTATCATGTCtc > 2:974616/1‑75 (MQ=18) gATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCtc > 2:938096/1‑75 (MQ=21) gATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa < 2:4161577/76‑1 (MQ=18) gATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa < 2:1627324/76‑1 (MQ=21) gATAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa < 2:2566370/76‑1 (MQ=21) gATAAGGCGCGCGCG‑CGGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa < 2:775602/76‑1 (MQ=21) tAAGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACt < 1:1994800/76‑1 (MQ=21) aaGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg < 2:1417574/76‑1 (MQ=21) aaGGCGCTCGAGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg > 2:871565/1‑76 (MQ=21) aGGCGCTCGCGCC‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg > 2:984341/1‑76 (MQ=21) gcgcgcg‑c‑C‑GCATCCGGCGATGGGGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGc < 2:2142809/76‑1 (MQ=25) | AAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCG‑G‑C‑GCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGC > NC_000913/4448478‑4448610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |