Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,826	+A	intergenic (+1/+44)	yjgL → / ← argI	hypothetical protein/ornithine carbamoyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,823	1	.	A	100.0%	196.5 / NA	62	*617K (TAA→AAA)	yjgL	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (34/28);  total (34/28)

AATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCA  >  NC_000913/4474748‑4474892                                                                             |                                                                      aataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat                                                                        <  2:1739131/76‑1 (MQ=255) aataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat                                                                        <  1:1667289/76‑1 (MQ=255) aataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTAGAAGAATAGACCTATCACTGCat                                                                        <  2:71594/76‑1 (MQ=255) aataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTGTGGGTGGAAGAATAGACCTATCACTGCat                                                                        <  2:1740428/76‑1 (MQ=255)      aCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaat                                                                  >  1:2865373/1‑74 (MQ=255)          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                                                               >  2:3167670/1‑76 (MQ=255)          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                                                               >  2:2515971/1‑76 (MQ=255)           aTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgt                                                              <  2:2530627/76‑1 (MQ=255)            tCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg                                                             >  2:3685097/1‑76 (MQ=255)            tCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg                                                             <  2:2003986/76‑1 (MQ=255)             ccACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGa                                                            >  1:1733867/1‑76 (MQ=255)               aCCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                          <  2:1675142/76‑1 (MQ=255)               aCCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                          >  2:478110/1‑76 (MQ=255)               aCCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                          >  2:539220/1‑76 (MQ=255)                 cGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc                                                        >  1:1637439/1‑76 (MQ=255)                 cGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc                                                        >  1:1879786/1‑76 (MQ=255)                  ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCg                                                       <  1:3835623/76‑1 (MQ=255)                  ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCg                                                       <  1:3822379/76‑1 (MQ=255)                  ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCg                                                       >  1:2053567/1‑76 (MQ=255)                       aTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATg                                                  >  1:1422268/1‑76 (MQ=255)                           tcGTATAGCAATAGAGCACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                              <  2:1387585/75‑1 (MQ=255)                            cGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTg                                             >  2:1241099‑M1/1‑75 (MQ=255)                              tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtt                                           >  2:236502‑M1/1‑73 (MQ=255)                                  gCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttc                                       <  1:3993956‑M1/76‑8 (MQ=255)                                       aGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggc                                  <  2:1047500‑M1/76‑13 (MQ=255)                                          acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                               >  1:2097117‑M1/1‑61 (MQ=255)                                          acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                               <  2:2158111‑M1/76‑16 (MQ=255)                                          acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                               <  2:3487283‑M1/76‑16 (MQ=255)                                          acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                               <  1:2618755‑M1/76‑16 (MQ=255)                                            acTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                             <  1:3451868‑M1/76‑18 (MQ=255)                                            acTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                             <  2:446743‑M1/76‑18 (MQ=255)                                            acTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                             >  1:446743‑M1/1‑59 (MQ=255)                                             cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttt                            >  2:3218506‑M1/1‑58 (MQ=255)                                             cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGGGATGCCGGGATGGTTTgtatttcccggcatcttt                            <  2:36128‑M1/76‑19 (MQ=255)                                              tttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                           >  2:3885876‑M1/1‑57 (MQ=255)                                              tttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcacggcatcttta                           >  1:2297336‑M1/1‑57 (MQ=255)                                               ttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                          >  1:1151987‑M1/1‑56 (MQ=255)                                               ttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                          <  2:4184791‑M1/76‑21 (MQ=255)                                               ttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                          >  1:2685230‑M1/1‑56 (MQ=255)                                                tGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                         <  1:4309962‑M1/76‑22 (MQ=255)                                                  ggTGGAAGAATAGACCTATCGCTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagc                       <  1:4401271‑M1/76‑24 (MQ=255)                                                   gTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                      >  2:4092234‑M1/1‑52 (MQ=255)                                                    tGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcga                     <  2:2971273‑M1/76‑26 (MQ=255)                                                    tGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcga                     <  2:2344632‑M1/76‑26 (MQ=255)                                                     ggAAGAATAGCCCTATCACTGCATAAAATAATGTGATGCCGGGATTTTTTgtatttcccggcatctttatagcgat                    <  2:718477‑M1/76‑27 (MQ=255)                                                     ggAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                    <  2:693002‑M1/76‑27 (MQ=255)                                                      gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                   >  2:1807712‑M1/1‑49 (MQ=255)                                                      gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                   >  2:1664054‑M1/1‑49 (MQ=255)                                                      gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                   >  1:199449‑M1/1‑49 (MQ=255)                                                      gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                   >  1:3599263‑M1/1‑49 (MQ=255)                                                         gaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                <  1:1664055‑M1/76‑31 (MQ=255)                                                          aaTAGACCTATCACTGCATAAAATAATGTGAGGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaa               >  1:901014‑M1/1‑45 (MQ=255)                                                           aTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat              <  2:1812109‑M1/76‑33 (MQ=255)                                                           aTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat              >  2:3580209‑M1/1‑44 (MQ=255)                                                             aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatta            >  1:4688486‑M1/1‑42 (MQ=255)                                                              gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcgcggcatctttatagcgatagcaattat           >  2:1098299‑M1/1‑41 (MQ=255)                                                                 cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattta        >  1:1305002‑M1/1‑38 (MQ=255)                                                                 cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattta        <  1:4153838‑M1/76‑39 (MQ=255)                                                                   aTCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact      >  2:24157‑M1/1‑36 (MQ=255)                                                                    tCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg     <  1:3685097‑M1/76‑42 (MQ=255)                                                                    tCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg     <  2:2303645‑M1/76‑42 (MQ=255)                                                                     cACTGCATAAAATAATGTGATGCCGGGATGGCTTgtattccccggcatctttatagcgaaagcaattatttactga    >  2:370768‑M1/1‑34 (MQ=255)                                                                      aCTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag   >  1:3454385‑M1/1‑33 (MQ=255)                                                                      aCTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag   >  1:3746363‑M1/1‑33 (MQ=255)                                                                       cTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc  <  2:4363635‑M1/76‑45 (MQ=255)                                                                       cTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc  <  1:3302797‑M1/76‑45 (MQ=255)                                                                       cTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc  >  1:2850211‑M1/1‑32 (MQ=255)                                                                             |                                                                      AATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCA  >  NC_000913/4474748‑4474892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑