Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,020	+G	intergenic (‑144/+65)	ypjA ← / ← b2648	putative ATP‑binding component of a transport system/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,019	1	.	G	100.0%	162.3 / NA	65	intergenic (‑143/+66)	ypjA/b2648	putative ATP‑binding component of a transport system/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (32/33);  total (32/33)

GTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCAC  >  NC_000913/2780944‑2781088                                                                             |                                                                      gTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                        >  1:1466300/1‑76 (MQ=255) gTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                        >  1:270523/1‑76 (MQ=255)  tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                      <  1:1350345/76‑1 (MQ=255)  tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                      <  2:3944912/76‑1 (MQ=255)     gcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑ggcc                                                                   >  2:3978843/1‑73 (MQ=255)     gcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑ggca                                                                   <  2:791911/76‑4 (MQ=255)        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGtt                                                                 >  2:1511797/1‑76 (MQ=255)        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGtt                                                                 >  1:3252017/1‑76 (MQ=255)          aGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTgg                                                               >  2:2928671/1‑75 (MQ=255)          aGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTgg                                                               <  1:3340737/76‑2 (MQ=255)           gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggc                                                              >  2:1391537/1‑74 (MQ=255)           gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggc                                                              <  2:2618540/76‑3 (MQ=255)           gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggc                                                              >  1:152921/1‑74 (MQ=255)            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggca                                                             <  2:4592985/76‑4 (MQ=39)            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggca                                                             >  2:4118652/1‑73 (MQ=39)            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggca                                                             <  2:3143603/76‑4 (MQ=39)            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggca                                                             <  2:2479656/76‑4 (MQ=39)            tgtgGTTGGATAAAAAAGTCAAGCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCa                                                             >  2:4130688/1‑76 (MQ=38)             gtgGTTGGATAAAAAAGTCAATCCATTCAGGAACTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                            <  1:4709750/76‑1 (MQ=38)              tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaa                                                           >  2:1005755/1‑76 (MQ=38)              tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaa                                                           <  2:2633455/76‑1 (MQ=38)               gtttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                          >  1:3688103/3‑76 (MQ=38)               ggTTGGATAGAAAAGTCAAGCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                          <  2:1875448/76‑1 (MQ=37)               ggTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                          <  1:3367493/76‑1 (MQ=38)               ggTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTAGTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                          <  2:1514314/76‑1 (MQ=37)                gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaat                                                         >  2:2024836/1‑76 (MQ=38)                gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaat                                                         <  1:1370716/76‑1 (MQ=38)                 ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTCACaaaata                                                        >  2:4779150/1‑76 (MQ=255)                      tAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATg                                                   <  2:1514801/76‑1 (MQ=37)                       aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                  >  1:1664886/1‑76 (MQ=37)                       aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                  <  1:2352269/76‑1 (MQ=37)                       aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                  <  1:2586844/76‑1 (MQ=37)                        aaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                 >  2:700313/1‑76 (MQ=37)                           aaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTa                                              >  2:150108/1‑76 (MQ=25)                             gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACa                                            <  1:4317170/76‑1 (MQ=37)                               cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                          <  2:4567656/76‑1 (MQ=37)                               cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                          <  1:1621521/76‑1 (MQ=37)                               cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                          <  1:4062527/76‑1 (MQ=37)                                aaTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATaa                                         >  2:992557/1‑76 (MQ=37)                                 aTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                        <  2:2647703/76‑1 (MQ=37)                                 aTCCATTCAGGAAATACGGGCGGATTCTTTTCTTTCGACAATGAGGCCGGTGGCAAAATAAAATGATTTACATAAt                                        <  2:2657467/76‑1 (MQ=25)                                  tCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                       >  1:4759706/1‑76 (MQ=37)                                  tCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                       >  1:1425451/1‑76 (MQ=37)                                   ccATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCg                                      <  1:4269728/76‑1 (MQ=37)                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGt                                     >  2:3304146/1‑76 (MQ=37)                                      ttCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGttt                                   <  2:357527/76‑1 (MQ=37)                                      ttCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGttt                                   <  2:3443548/76‑1 (MQ=37)                                      ttCAGGAAATACGGGAGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAGCGgtt                                   >  1:794848/1‑76 (MQ=21)                                          ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtga                               <  1:2491662/76‑1 (MQ=25)                                            aaaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatg                             <  1:1011427/76‑1 (MQ=25)                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                           <  2:4136693/76‑1 (MQ=25)                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                           >  1:2721064/1‑76 (MQ=25)                                               ttcGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAat                          <  1:241476/74‑1 (MQ=21)                                                 cGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAatat                        >  1:2025962/1‑76 (MQ=25)                                                 cGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAatat                        <  1:3468285/76‑1 (MQ=25)                                                 cGGGCGTATTCTTGTCTTTCGACAATGAGGCCGTGGGCAAAATAAAAGGATTTACATAATCGTTTCTGATGAatat                        <  1:3490809/76‑1 (MQ=18)                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCt                >  1:1767398/1‑76 (MQ=18)                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTc               >  1:1968993/1‑76 (MQ=18)                                                           cttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATAGTTTCTGATGAATATCTTCTGCTca              >  1:625621/1‑76 (MQ=18)                                                             tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcaca            <  1:2152662/76‑1 (MQ=17)                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaa         >  2:2592587/1‑76 (MQ=17)                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaa         >  2:2610052/1‑76 (MQ=17)                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaa        >  2:1843970/1‑76 (MQ=17)                                                                 tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaa        >  1:2292511/1‑76 (MQ=17)                                                                  ttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaaa       >  1:4113533/1‑76 (MQ=17)                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaaaa      >  2:3897203/1‑76 (MQ=17)                                                                    cGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAt     >  2:2436036/1‑76 (MQ=17)                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc    <  1:4436922/76‑1 (MQ=17)                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc    <  2:940587/76‑1 (MQ=17)                                                                      aCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATca   <  2:3076437/76‑1 (MQ=17)                                                                       cAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcac  >  2:2961540/1‑76 (MQ=17)                                                                             |                                                                      GTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCAC  >  NC_000913/2780944‑2781088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑