BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,044	+G	coding (498/1458 nt)	ascF →	PTS system enzyme IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,043	1	.	G	100.0%	252.4 / NA	94	A166G (GCG→GGG)	ascF	PTS system enzyme IIBC
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (41/53); total (41/53)

Only 100 of 102 total aligned reads displayed.

CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATC  >  NC_000913/2837968‑2838114

cAAAAGTATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc                                                                          <  1:255581/76‑1 (MQ=255)

cAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc                                                                          <  2:504316/76‑1 (MQ=255)

 aaaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑g                                                                        <  2:2143059/76‑1 (MQ=255)

 aaaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑g                                                                        <  1:3880760/76‑1 (MQ=255)

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑gg                                                                       >  1:4292589/1‑75 (MQ=255)

   aaGGGTCGCCGCCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑ggc                                                                      <  2:738526/76‑3 (MQ=255)

   aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑ggc                                                                      >  1:591146/1‑74 (MQ=255)

    aGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcgc‑ggca                                                                     <  2:1292057/76‑4 (MQ=255)

       aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCt                                                                   <  2:3476486/76‑1 (MQ=255)

       aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCt                                                                   <  2:4253930/76‑1 (MQ=255)

       aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCt                                                                   <  1:1608352/76‑1 (MQ=255)

       aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCt                                                                   <  2:4666051/76‑1 (MQ=255)

        tCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTg                                                                  >  2:4139941/1‑76 (MQ=255)

         cGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGc                                                                 >  1:2794523/1‑76 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGcc                                                                <  2:3838648/76‑1 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGcc                                                                >  1:1765119/1‑76 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGcc                                                                >  2:2092036/1‑76 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCCGATGGTCGCGGCATCTGcc                                                                <  1:3944633/76‑1 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATGGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGcc                                                                <  2:1059701/76‑1 (MQ=255)

          cccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGcc                                                                <  1:1603202/75‑1 (MQ=255)

           ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcg                                                               >  1:765965/1‑76 (MQ=255)

           ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCGTCTTCCTGCCGCTCATGGTCCCGGCATCTGCcg                                                               >  1:779194/1‑76 (MQ=255)

            cGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgc                                                              <  1:4635098/76‑1 (MQ=255)

            cGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgc                                                              >  1:1067574/1‑76 (MQ=255)

            cGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgc                                                              >  1:3148130/1‑76 (MQ=255)

              cccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgcca                                                            <  1:1828411/75‑3 (MQ=255)

              aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgcca                                                            <  2:3471506/76‑3 (MQ=255)

              aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgcca                                                            >  2:847159/1‑74 (MQ=255)

               ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgccct                                                           >  2:2067645/1‑73 (MQ=255)

               ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCcgccat                                                           >  1:4472788/1‑73 (MQ=255)

                 ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCa                                                        <  1:1296700/76‑1 (MQ=255)

                  tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaa                                                       <  1:2981009/76‑1 (MQ=255)

                  tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaa                                                       >  1:999962/1‑76 (MQ=255)

                  tAACCATTCTGAATGGGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaa                                                       <  2:874067/76‑1 (MQ=255)

                   aaCCATTCTGAATGTGATTGGTGACTGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCaaa                                                      <  2:3207135/76‑1 (MQ=255)

                    aCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAt                                                     >  1:3793513/1‑76 (MQ=255)

                     ccATTCTGAATGTGATTGGGGACGGGGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAtt                                                    >  1:3484427/1‑76 (MQ=255)

                      cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAttt                                                   <  1:4455715/76‑1 (MQ=255)

                      cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAAttt                                                   <  1:3706657/76‑1 (MQ=255)

                       aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTa                                                  <  2:2454093/76‑1 (MQ=255)

                        ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                 >  1:613431/1‑76 (MQ=255)

                        ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                 <  1:3036212/76‑1 (MQ=255)

                        ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                 <  2:3878653/76‑1 (MQ=255)

                        ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaa                                                 <  1:3768307/76‑1 (MQ=255)

                         tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaaa                                                <  1:217899/76‑1 (MQ=255)

                          cTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTaaaa                                               <  1:2638000/76‑1 (MQ=255)

                           tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAc                                              <  2:3637707/76‑1 (MQ=255)

                            gAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAcc                                             >  2:3543338/1‑76 (MQ=255)

                            gAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAAcc                                             >  1:1699902/1‑76 (MQ=255)

                             aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCa                                            <  2:2523000/76‑1 (MQ=255)

                              aTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                           >  2:461312/1‑76 (MQ=255)

                               tgtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                          <  1:3077426/76‑1 (MQ=255)

                                gtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                         <  2:3072764/76‑1 (MQ=255)

                                  ggttGGTGCCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                       <  2:1763100/74‑1 (MQ=255)

                                  gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                       >  2:3547640/1‑76 (MQ=255)

                                   gttGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                      <  2:3251922/75‑1 (MQ=255)

                                     tGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                    <  2:579995/76‑1 (MQ=255)

                                     tGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                    <  2:4420961/76‑1 (MQ=255)

                                      ggTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                   >  2:3111124/1‑76 (MQ=255)

                                      ggTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                   <  1:978791/76‑1 (MQ=255)

                                        tGACGGTGTTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                 <  2:2304784/76‑1 (MQ=255)

                                        tGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                 <  1:2127254/76‑1 (MQ=255)

                                        tGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                 >  1:2740332/1‑76 (MQ=255)

                                        tGACGGTGCTTTCTTCTCCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                 <  2:4332591/76‑1 (MQ=255)

                                        tGACGATGCTTTCTTCTCCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAGCCAATAGGTCGCTg                                 <  2:3685766/76‑1 (MQ=255)

                                         gACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgg                                <  1:2118851/76‑1 (MQ=255)

                                          aCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgtc                               >  1:3076626/1‑74 (MQ=255)

                                           cGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCg                              <  1:626662/76‑1 (MQ=255)

                                               gCTTTCTTCTTCCGGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTg                          <  2:4096696/76‑1 (MQ=255)

                                                cTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGc                         >  1:4324058/1‑76 (MQ=255)

                                                cTTTCTTCTGCCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGc                         >  2:2841257/1‑76 (MQ=255)

                                                  ttcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                       >  1:1204281/1‑76 (MQ=255)

                                                   tcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                      <  1:3513670/76‑1 (MQ=255)

                                                   tcttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                      <  1:266810/76‑1 (MQ=255)

                                                     ttcttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcg                    >  2:1239943/1‑76 (MQ=255)

                                                       cttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGCTTGCCATctcg                  >  2:910995/1‑76 (MQ=255)

                                                       cttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgcg                  <  1:3823850/76‑1 (MQ=255)

                                                       cttcCTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgcg                  >  1:3241408/1‑76 (MQ=255)

                                                       ctcccTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgcg                  <  1:139371/73‑1 (MQ=255)

                                                           cTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtg              >  1:2746657/1‑76 (MQ=255)

                                                           cTGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtg              <  1:2841257/76‑1 (MQ=255)

                                                            tGCCGCTGATGGTGGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAGCGCGGgtgt             <  1:1186401/76‑1 (MQ=255)

                                                            tGCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtgg             >  1:2299922/1‑75 (MQ=255)

                                                            cgCCGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAACCCAATATGTCGCTGGCGATTGCCATCGCGGgtgt             <  1:1985620/75‑1 (MQ=255)

                                                              ccGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCCTCGCGGGGGTGc           >  2:204089/1‑76 (MQ=255)

                                                               cGCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTCAAACCAATATGTCGCTGGCGATTGCCCTCGCGGGGGTGCt          >  2:2855938/1‑76 (MQ=255)

                                                                gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg         >  2:4402161/1‑76 (MQ=255)

                                                                gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg         <  2:4416321/76‑1 (MQ=255)

                                                                gCTGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg         >  1:3886689/1‑76 (MQ=255)

                                                                  tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt       <  1:1288219/76‑1 (MQ=255)

                                                                  tGATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt       <  1:3850817/76‑1 (MQ=255)

                                                                  tGATGGGCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt       <  2:278859/76‑1 (MQ=255)

                                                                   gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa      >  2:2076758/1‑76 (MQ=255)

                                                                   gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa      >  2:2541400/1‑76 (MQ=255)

                                                                   gATGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGGGtggtgggg      >  2:751686/1‑74 (MQ=255)

                                                                    agggTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTAc     <  2:225647/74‑1 (MQ=255)

                                                                     tGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACa    >  1:4073616/1‑76 (MQ=255)

                                                                     tGGTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACa    <  1:2731360/76‑1 (MQ=255)

                                                                       gTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc  >  2:914573/1‑76 (MQ=255)

                                                                       gTCGCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGGGCTGGTACCTc  >  2:76469/1‑76 (MQ=255)

CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATC  >  NC_000913/2837968‑2838114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`