| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,005,399 | +C | coding (1044/1092 nt) | ygeW → | putative carbamoyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,005,398 | 1 | . | C | 99.2% | 332.5 / ‑3.6 | 129 | coding (1043/1092 nt) | ygeW | putative carbamoyl transferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (45/83); total (46/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 153 total aligned reads displayed.
AAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑CTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGA > NC_000913/3005323‑3005468 | aaGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCgatga‑t > 1:1340066/1‑76 (MQ=255) aGGCTTATACGAAAAATACCGTATCTCTACCTACAAAGAAGCCATCTGGAAGCCTTTTATCATCGCCGCGATGA‑T‑c < 2:4327361/76‑1 (MQ=255) aGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑c < 2:1802603/76‑1 (MQ=255) gCGTATTCGAAAAATACCGTATCGCTACCTACTAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑cct < 1:1041066/76‑3 (MQ=255) gCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑cct > 1:3848111/1‑74 (MQ=255) gCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑cct > 2:1376419/1‑74 (MQ=255) gCGTATTCGAAAAATACCGGATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑cct > 1:4534491/1‑74 (MQ=255) cGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑cctg < 2:2467424/76‑4 (MQ=255) cGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑cctg < 1:823201/76‑4 (MQ=255) cGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑cctg > 1:2027561/1‑73 (MQ=255) aTTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTcc < 1:2619904/76‑1 (MQ=255) aTTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTcc < 1:4384392/76‑1 (MQ=255) aTTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTcc > 2:751009/1‑76 (MQ=255) tCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCg > 1:2734550/1‑76 (MQ=255) cGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGt > 2:4039466/1‑76 (MQ=255) cGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGt > 1:290979/1‑76 (MQ=255) gAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTa > 1:1507374/1‑76 (MQ=255) gAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTa > 1:946437/1‑76 (MQ=255) aaaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaa > 1:4010662/1‑76 (MQ=255) aaaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaa < 1:266748/76‑1 (MQ=255) aaaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaa < 2:3153185/76‑1 (MQ=255) aaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaaa < 1:172280/76‑1 (MQ=255) aaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaaa < 2:4069914/76‑1 (MQ=255) aaaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTaaa < 1:656253/76‑1 (MQ=255) aaaTCCCGTATCGCTGCCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAAt < 2:4691903/76‑1 (MQ=255) aaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAAt < 2:3143730/76‑1 (MQ=255) aaaTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAAt > 1:1384799/1‑76 (MQ=255) aTACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATAc < 2:2304119/76‑1 (MQ=255) tACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACg < 1:2021042/76‑1 (MQ=255) acccGTACCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACg < 2:696828/74‑1 (MQ=255) aCCGTATCGCTACGTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGc < 2:1229888/76‑1 (MQ=255) ccGGATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGcc < 2:644455/76‑1 (MQ=255) cGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCa > 1:4621313/1‑76 (MQ=255) tATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCaaa < 2:1993490/76‑1 (MQ=255) tATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCaaa < 2:886310/76‑1 (MQ=255) aTCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAc < 2:1588167/76‑1 (MQ=255) tCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAcc < 2:3611888/76‑1 (MQ=255) tCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAcc > 1:656050/1‑76 (MQ=255) tCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAAcc < 1:3001792/76‑1 (MQ=255) cGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCa < 1:3613632/76‑1 (MQ=255) cGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCa < 1:216873/76‑1 (MQ=255) catacctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAg < 2:4590590/74‑1 (MQ=255) tacctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGt > 1:3564938/1‑76 (MQ=255) tacctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGt < 1:4700278/76‑1 (MQ=255) acctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTg < 1:3338982/76‑1 (MQ=255) cctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGc < 2:2469314/76‑1 (MQ=255) cctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGc < 2:2502560/76‑1 (MQ=255) cctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGc < 1:3262013/76‑1 (MQ=255) ctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCa > 1:3245457/1‑76 (MQ=255) acAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACt < 1:4254841/76‑1 (MQ=255) cAAAGGAGCCAGCTGGAGGCCTTATAGCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg < 2:4301009/76‑1 (MQ=255) cAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACtc > 2:563671/1‑75 (MQ=255) cAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg < 1:3039993/76‑1 (MQ=255) cAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg < 2:1227015/76‑1 (MQ=255) aaaaGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTg < 1:139982/75‑1 (MQ=255) aaaGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGc < 2:1465614/76‑1 (MQ=255) agaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTc > 1:4191050/1‑76 (MQ=255) aagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGa < 1:3181067/76‑1 (MQ=255) aagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGa < 2:1694338/76‑1 (MQ=255) aagCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGa > 1:1278213/1‑76 (MQ=255) aGCTGGAAGCCTTATATCATCGCCGCGGTGAGA‑CTGGCACGTTAATACGCCAAAACAGGCGCAGTGCTAGaacaac > 1:562126/1‑76 (MQ=255) aGCTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAAc < 2:2229088/76‑1 (MQ=255) gccggAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAAct < 2:333012/73‑1 (MQ=255) cTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActg < 2:1112411/76‑1 (MQ=255) cTGGAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActg > 1:771542/1‑76 (MQ=255) tGGAAGTCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgc > 1:1633217/1‑76 (MQ=255) ggAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgct > 1:3891009/1‑76 (MQ=255) ggAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgct > 2:1810070/1‑76 (MQ=255) gAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgctg < 1:1542621/76‑1 (MQ=255) gAAGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGCGCCCCGCTCGAGCAActgctg > 2:331876/1‑76 (MQ=255) aGCCTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaa < 2:1507373/76‑1 (MQ=255) ccTTGTGTCAGCGCCGGGGTGA‑GCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCACCTGCTGAaag < 2:251920/76‑1 (MQ=255) cTTATATCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaaga > 2:3219750/1‑76 (MQ=255) tataTCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaagaag > 1:2495342/1‑76 (MQ=255) atatCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc < 1:2246/76‑1 (MQ=255) atatCATCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc > 2:4167268/1‑76 (MQ=255) tatCAGCGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgcg < 1:4821895/76‑1 (MQ=255) atcatcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgcgc > 2:1622561/1‑76 (MQ=255) atcatcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgcgc < 2:4068532/76‑1 (MQ=255) tcatcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCa < 2:2096536/76‑1 (MQ=255) catcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCaa > 1:3187712/1‑76 (MQ=255) catcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCaa > 1:1112508/1‑76 (MQ=255) catcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCaa > 2:1717935/1‑76 (MQ=255) atcTCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAg < 2:4834746/76‑1 (MQ=255) tcGCCGCGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCACCTGCTGAAAGAAGCGCACGa < 2:2002511/76‑1 (MQ=255) cgccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGaa < 2:3918232/76‑1 (MQ=255) cgccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGaa < 2:3245457/76‑1 (MQ=255) gccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAc > 2:101213/1‑76 (MQ=255) ccgcGATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcg < 2:797724/76‑1 (MQ=255) cgcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGGAACTGCTGAAAGAAGCGCAAGAAcgc < 2:1376900/76‑1 (MQ=255) cgcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgc < 1:3788678/76‑1 (MQ=255) cgcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgc > 2:3867884/1‑76 (MQ=255) gcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgcg < 2:3012431/76‑1 (MQ=255) gcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgcg > 2:2672012/1‑76 (MQ=255) gcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgcg > 1:4535335/1‑76 (MQ=255) gcgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgcg < 1:1877120/76‑1 (MQ=255) tgatga‑tcCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGt > 2:4098886/2‑76 (MQ=255) cgATGA‑TCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGt < 1:3607991/76‑1 (MQ=255) gatga‑tcCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTg > 1:1602039/1‑76 (MQ=255) gatga‑tcCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTg > 2:3692350/1‑76 (MQ=255) | AAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGA‑T‑CTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGA > NC_000913/3005323‑3005468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |