Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,640	+G	intergenic (‑291/+23)	cmtB ← / ← tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,640	1	.	G	100.0%	190.5 / NA	73	intergenic (‑291/+23)	cmtB/tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (49/24);  total (49/24)

TGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGC  >  NC_000913/3077565‑3077709                                                                             |                                                                      tgtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGc                                                                        <  1:2972363/76‑1 (MQ=255)  gtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcg                                                                       >  2:559116/1‑75 (MQ=255)  gtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcg                                                                       >  1:3890171/1‑75 (MQ=255)  gtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcg                                                                       >  1:2192351/1‑75 (MQ=255)       cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                  >  1:4466881/1‑76 (MQ=255)        tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                 >  1:265543/1‑76 (MQ=255)         acaacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                <  2:409989/76‑1 (MQ=255)              acGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGACGCGACCTTTTTAccc                                                           <  2:96699/76‑1 (MQ=255)              acGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAccc                                                           >  1:452560/1‑76 (MQ=255)               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttttt                                                               <  1:447601/71‑1 (MQ=255)               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttttt                                                               >  2:447601/1‑71 (MQ=255)               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCg                                                          >  2:2600294/1‑76 (MQ=255)                tccTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGTCCTTTTTACCCGa                                                         <  1:3379600/75‑1 (MQ=255)                 ccTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaa                                                        >  2:3399514/1‑76 (MQ=255)                  cTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaaa                                                       <  2:342042/76‑1 (MQ=255)                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAt                                                      <  1:2201982/76‑1 (MQ=255)                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAt                                                      >  1:3914545/1‑76 (MQ=255)                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAACGTCGCCGAAGCGACCTTTTTACCCGAAAt                                                      >  1:3129588/1‑76 (MQ=255)                    tATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATg                                                     >  1:3513245/1‑76 (MQ=255)                     aTCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGc                                                    >  1:693808/1‑76 (MQ=255)                        tATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTaa                                                 >  2:3420492/1‑76 (MQ=255)                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                >  1:2728943/1‑76 (MQ=255)                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                <  2:3362407/76‑1 (MQ=255)                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                <  1:4450873/76‑1 (MQ=255)                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                <  1:4487756/76‑1 (MQ=255)                           tGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTa                                              >  1:2845068/1‑76 (MQ=255)                            gCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc                                             >  2:2247729/1‑76 (MQ=255)                             cTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAca                                            <  1:4408938/76‑1 (MQ=255)                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcag                                           >  1:2195416/1‑76 (MQ=255)                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcag                                           <  1:4666729/76‑1 (MQ=255)                               tGTCCGCAAACGGACATACCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagc                                          >  2:1668211/1‑76 (MQ=255)                                  ccGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGt                                       >  1:2498589/1‑76 (MQ=255)                                   cGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCTGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGtt                                      <  2:2430441/76‑1 (MQ=255)                                    gCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTCTTTACCCGAAATGCTAATTACAGCAGTTc                                     >  2:2521912/1‑76 (MQ=255)                                         cGGCCATATAAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGATAATTACAGCAGTTCTCTTg                                >  2:375201/1‑76 (MQ=255)                                         cGGACATATCAAGGCAATAAAAAAGGTCGCCGAAGCGACCTCTTTACCCGAAATGCTAATTACAGCAGTTCTTTTg                                >  2:4207733/1‑76 (MQ=255)                                          tgACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                               >  2:1891808/2‑76 (MQ=255)                                          ggACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                               >  2:505266/1‑76 (MQ=255)                                            aCATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGCAATGCTAATTACAGCAGTTCTTTTGCtt                             >  2:1006884/1‑76 (MQ=255)                                              atatCAAGGTAATAAAAAAGGTCGCCGAAGCGGCCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTc                           <  2:154918/76‑1 (MQ=255)                                                 tCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGca                        <  1:1426840/76‑1 (MQ=255)                                                    aGGTCATAGAGACGGTCGCCGAACCGACGTTTTTGCCCGAGATGCTAATTACAGCAGTTCTTTTGCTTTCGcacga                     <  2:2552619/76‑4 (MQ=255)                                                    aGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaaca                     >  2:3384756/1‑76 (MQ=255)                                                    aGGTAATAAAAAAGGTCGCCGAAGCGACCGTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaaca                     <  2:2543090/76‑1 (MQ=255)                                                     ggTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaacaa                    >  1:1037040/1‑76 (MQ=255)                                                      gTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCaacaac                   >  1:1708976/1‑76 (MQ=255)                                                       taataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACg                  >  1:2494193/1‑76 (MQ=255)                                                         ataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGtt                >  2:1315778/1‑76 (MQ=255)                                                          taaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTa               >  2:4375652/1‑76 (MQ=255)                                                           aaaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACACCGCTAt              >  2:2908172/1‑76 (MQ=255)                                                           aaaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAt              >  2:222054/1‑76 (MQ=255)                                                            aaaaaGGTCGCCGAAGCGACCTTTTTACCCGACATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc             >  2:2520437/1‑76 (MQ=255)                                                            aaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc             >  2:2549641/1‑76 (MQ=255)                                                            aaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc             >  2:3847043/1‑76 (MQ=255)                                                             aaaaGGTCGCCGAAGCGACCTTATTACCCGAAATGCCAATTACAGCAGTTCTTTTGCGTTCGCAACAACGCTATCa            >  2:3192366/1‑76 (MQ=255)                                                              aaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCaa           >  1:794916/1‑76 (MQ=255)                                                               aaGGTCGCCGAGGCGACCTTTTTGCCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc          <  2:2539152/76‑1 (MQ=255)                                                               aaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc          >  1:1757013/1‑76 (MQ=255)                                                               aaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc          <  1:3251211/76‑1 (MQ=255)                                                                aGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCACCAACGTTATCAACa         >  2:3372148/1‑76 (MQ=255)                                                                aGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACa         <  2:943612/76‑1 (MQ=255)                                                                 ggTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAg        <  2:2498589/76‑1 (MQ=255)                                                                  gTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt       <  2:1938201/76‑1 (MQ=255)                                                                  gTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt       <  2:2252053/76‑1 (MQ=255)                                                                   tCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg      >  2:3940196/1‑76 (MQ=255)                                                                   tCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg      >  1:28071/1‑76 (MQ=255)                                                                   tCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg      >  1:4270182/1‑76 (MQ=255)                                                                   tCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg      <  2:655184/76‑1 (MQ=255)                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCACCAGTGaa    >  2:309639/1‑76 (MQ=255)                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa    >  1:1798061/1‑76 (MQ=255)                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa    >  2:551841/1‑76 (MQ=255)                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa    >  2:2328631/1‑76 (MQ=255)                                                                      ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg   <  1:2944647/76‑1 (MQ=255)                                                                      ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg   >  2:1149421/1‑76 (MQ=255)                                                                       cGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc  >  1:3382529/1‑76 (MQ=255)                                                                       cGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc  >  1:3724928/1‑76 (MQ=255)                                                                       cGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc  >  2:977188/1‑76 (MQ=255)                                                                             |                                                                      TGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGC  >  NC_000913/3077565‑3077709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑