| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,256,893 | +C | coding (405/423 nt) | yhaQ ← | putative L‑serine dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,256,891 | 1 | . | C | 97.4% | 298.8 / 2.6 | 115 | coding (407/423 nt) | yhaQ | putative L‑serine dehydratase |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (56/56); total (58/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT > NC_000913/3256816‑3256961 | cAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑Gt > 1:4569987/1‑76 (MQ=255)cAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑Gt > 1:1836498/1‑76 (MQ=255) aGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GT‑c > 2:635033/1‑76 (MQ=255) ggCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GT‑cc > 2:3071397/1‑76 (MQ=255) gCCGAAGTGTTCTTCTTCGACAATAAGCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GT‑ccc > 1:1374553/1‑75 (MQ=255) gCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GT‑ccc > 2:4191055/1‑75 (MQ=255) ccGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GT‑ccct < 2:2859294/76‑3 (MQ=255) gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAg < 2:1912314/76‑1 (MQ=255) gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAg > 2:3611270/1‑76 (MQ=255) tgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAgg < 1:2897694/76‑1 (MQ=255) gtTCTTCTTCGCCAAGAAACACGCCGCCGACAGAGTAATAGGTTTTACTTACAAGCTCGTCCTGGC‑CC‑TTCCAGGc > 1:4514014/1‑76 (MQ=255) gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGc < 1:3548270/76‑1 (MQ=255) gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGc > 1:3347788/1‑76 (MQ=255) ttcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCa < 1:4018596/76‑1 (MQ=255) ttcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCa > 1:4704630/1‑76 (MQ=255) cttcttcGACAGTAACCCCTCCGCCGACAGAGTGATAGGTTTTACTTGATGGCTCTTCCT‑GT‑CCGTTCCAGGCAGt < 2:860986/76‑1 (MQ=255) cttcttcGACAATAAACCCGCCGCCGCCAGAGTAATAGGTTTTACTTAATACCTCTTCCT‑TTCCC‑TTCCCGGcccg > 2:58796/1‑73 (MQ=255) ttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTg < 2:1851636/76‑1 (MQ=255) ttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTg > 1:3223267/1‑76 (MQ=255) tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGa < 1:266640/76‑1 (MQ=255) cttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGAt > 1:3587318/1‑76 (MQ=255) ttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGacc < 1:2321941/76‑3 (MQ=255) ttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATc > 1:2544044/1‑76 (MQ=255) ttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATc < 1:2962644/76‑1 (MQ=255) tcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATcc > 1:707155/1‑76 (MQ=255) gACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGc > 1:3202351/1‑76 (MQ=255) gACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGc > 2:4013063/1‑76 (MQ=255) aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCAtt > 2:3158995/1‑76 (MQ=255) aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCAtt < 1:1237442/76‑1 (MQ=255) aTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCCTTc > 2:233837/1‑76 (MQ=255) aTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTc < 2:1631194/76‑1 (MQ=255) tAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTcc > 1:2688780/1‑76 (MQ=255) aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGtt < 2:1930137/76‑1 (MQ=255) cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtc < 1:3503436/76‑1 (MQ=255) ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtct > 2:4055961/1‑76 (MQ=255) cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtctc > 1:3529156/1‑76 (MQ=255) gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa > 1:2417090/1‑76 (MQ=255) gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa > 1:1942806/1‑76 (MQ=255) gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa < 1:1863437/76‑1 (MQ=255) ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCAt > 1:4572724/1‑76 (MQ=255) cgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATg < 2:1740479/76‑1 (MQ=255) gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCTGTGATCCGCATTCCGTTCTCATgg > 1:1174782/1‑76 (MQ=255) gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATgg > 1:1029243/1‑76 (MQ=255) ccgACATAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCCGTGATCCGCATTCCGTTCTCATGGc < 2:3671562/76‑1 (MQ=255) ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGc > 2:1802555/1‑76 (MQ=255) cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TACCAGGCAGTGATCCGCATTCCGTTCTCATGGCg > 2:3574147/1‑76 (MQ=255) gACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGa < 2:4756259/76‑1 (MQ=255) gACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGa < 1:3326438/76‑1 (MQ=255) aCAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg > 1:2190671/1‑76 (MQ=255) agagTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGc > 1:3357989/1‑76 (MQ=255) agagTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGc > 1:4292714/1‑76 (MQ=255) gagTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCa > 1:2420363/1‑76 (MQ=255) gagTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCa > 2:2670726/1‑76 (MQ=255) gagTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCa < 1:277965/76‑1 (MQ=255) gagTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGACa > 2:1206780/1‑76 (MQ=255) agTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa > 2:2374430/1‑76 (MQ=255) agTAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa < 2:3072184/76‑1 (MQ=255) tAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa < 2:4616759/76‑1 (MQ=255) tAATAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa > 1:3275388/1‑76 (MQ=255) aTAGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt < 1:74294/76‑1 (MQ=255) aGGTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc < 1:2620250/76‑1 (MQ=255) aGGTTGTGCTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc < 2:1646019/76‑1 (MQ=255) aGGGTTTACTTAATAGCTCTTCCT‑GGCCC‑TTCCAGGCAGTGATCCGCATTCCCTGCGCATGGCGAGGCAACATTTc > 2:1348554/1‑76 (MQ=255) gggtttACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACCTTTCg > 2:4661470/4‑76 (MQ=255) ggTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg < 1:525880/76‑1 (MQ=255) ggTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg > 2:2304918/1‑76 (MQ=255) gTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg < 2:824747/76‑1 (MQ=255) gTTTTACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg < 2:1437430/76‑1 (MQ=255) ttttACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCggg < 2:3816911/76‑1 (MQ=255) tttACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGa < 1:4852677/76‑1 (MQ=255) ttACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAt < 2:3389083/76‑1 (MQ=255) ttACTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTCATCATGGCGAGGCAACATTTCGGGAt > 1:3965618/1‑76 (MQ=255) aCTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACCTTTCGGGATgg > 2:4173431/1‑76 (MQ=255) aCTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg > 2:2145691/1‑76 (MQ=255) cTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa > 1:2653977/1‑76 (MQ=255) cTTAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa < 1:1971462/76‑1 (MQ=255) cTTAATAGATCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa < 1:1984225/76‑1 (MQ=255) ttAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa > 1:3232065/1‑76 (MQ=255) ttAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa > 1:1770375/1‑76 (MQ=255) ttAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa < 2:1033431/76‑1 (MQ=255) tAATAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg < 2:168802/76‑1 (MQ=255) aaTAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGa > 1:1237567/1‑76 (MQ=255) aaTAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGa < 2:3648507/76‑1 (MQ=255) aTAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCTACATTTCGGGATGGAAGAt > 1:1942305/1‑76 (MQ=255) aTAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAt > 1:546392/1‑76 (MQ=255) tAGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATa > 1:667814/1‑76 (MQ=255) tAGCTCTTCCT‑GCCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGTGAGGCAACATTTCGGGATGGAAGATa > 2:4300907/1‑76 (MQ=255) aGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATaa > 2:1192042/1‑76 (MQ=255) aGCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATaa < 1:229281/76‑1 (MQ=255) gCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTTGGGATGGAAGATAAt < 2:3928980/76‑1 (MQ=255) gCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAt < 2:3685527/76‑1 (MQ=255) gCTCTTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAt < 1:3362607/76‑1 (MQ=255) ctctTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg < 1:4819738/76‑1 (MQ=255) tctTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGCTAATGt > 1:4061943/1‑76 (MQ=255) tctTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt < 2:4016319/76‑1 (MQ=255) tctTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt < 2:1718725/76‑1 (MQ=255) ctTCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt < 2:1033184/76‑1 (MQ=255) ttCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc < 2:3783031/76‑1 (MQ=255) ttCCT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc > 2:1796927/1‑76 (MQ=255) ccT‑GTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt < 1:3846112/76‑1 (MQ=255) | CAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCT‑GT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT > NC_000913/3256816‑3256961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |