| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,537,405 | +GC | intergenic (+61/‑394) | b3407 → / → feoA | hypothetical protein/ferrous iron transport protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,537,404 | 1 | . | C | 98.6% | 170.6 / ‑3.8 | 69 | intergenic (+60/‑395) | b3407/feoA | hypothetical protein/ferrous iron transport protein A |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (21/47); total (21/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,537,404 | 2 | . | G | 98.6% | 174.8 / ‑3.5 | 69 | intergenic (+60/‑395) | b3407/feoA | hypothetical protein/ferrous iron transport protein A |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (21/47); total (21/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA > NC_000913/3537329‑3537468 || gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑Ca > 1:4002281/1‑76 (MQ=255)gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑Ca > 2:2878587/1‑76 (MQ=255) tgCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑c > 2:3041947/2‑76 (MQ=255) ggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑c < 2:713511/76‑1 (MQ=255) cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑cgc < 2:4377369/76‑3 (MQ=255) aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑cgcc < 1:4849180/76‑4 (MQ=255) aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑cgcc < 2:2546973/76‑4 (MQ=255) aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGCAGGGCAGATAAAGCGTT‑‑CA‑‑cgcc > 1:2673450/1‑73 (MQ=255) gTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTg < 2:2917401/76‑1 (MQ=37) tAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGc < 1:2820820/76‑1 (MQ=37) tAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGc < 1:2976323/76‑1 (MQ=37) tAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGc < 2:2979591/76‑1 (MQ=37) aaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCaa < 1:4364844/76‑1 (MQ=25) acccccGTACGTGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCaac < 1:303693/73‑1 (MQ=21) aCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCaac < 2:4188871/76‑1 (MQ=25) cGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCaacg > 1:1018778/1‑76 (MQ=25) gagcGTTCTGGGGTACGGTTGGAATTTGCTCGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCaacga < 2:1866286/73‑1 (MQ=12) gCCCGTACTGGCCTACGGTTGGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCaacgg < 1:2887173/76‑2 (MQ=25) gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGc < 1:4382326/76‑1 (MQ=25) gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGc < 2:3680454/76‑1 (MQ=25) aCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCt > 2:1700362/1‑76 (MQ=25) tGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCttc > 2:4004602/1‑75 (MQ=25) ggCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGc < 2:2215673/76‑1 (MQ=25) ccgaCGGGTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCt < 2:296946/73‑1 (MQ=21) ccTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCt < 2:2544883/76‑1 (MQ=25) cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTg < 2:1731013/76‑1 (MQ=25) cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTg < 2:2867886/76‑1 (MQ=25) cTACGGTTCGAATTTGCACGAAATCGGAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTg < 2:4395866/76‑1 (MQ=25) tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGa < 1:2742892/76‑1 (MQ=25) tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGa > 1:175180/1‑76 (MQ=25) ggTTGGAATTTGAACGAAATCGTAGGGAAGATAAGGCGTT‑‑CACGCCGCATCTGGNNACGAACGCCTTGCCTGACAt < 1:265855/76‑1 (MQ=21) ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACAt > 2:2782120/1‑76 (MQ=25) ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACAt < 1:1870658/76‑1 (MQ=25) ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACAt > 2:3571238/1‑76 (MQ=25) ggTTCGAATTTGCACGAAAGCGAAGGGCAGACAAAGCGTT‑‑CACGCCGCATCTCGCAACGAACGCCTTGCCTGACaa > 1:2148667/1‑75 (MQ=255) gTTCGAATTTGCACTAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATa < 1:851999/76‑1 (MQ=25) ttCGACTTTGCACGAAATCGTAGGGCCGATAAGCCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaa < 1:38666/76‑1 (MQ=25) cGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaaaa < 1:4356463/76‑1 (MQ=25) gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg < 2:3179850/76‑1 (MQ=25) gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg < 2:3914725/76‑1 (MQ=25) aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt < 2:582900/76‑1 (MQ=25) aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt < 2:2862630/76‑1 (MQ=25) aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTCCCTGACATAAAAgg > 2:299648/1‑75 (MQ=25) aTTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg > 1:2382783/1‑76 (MQ=25) aTTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg < 2:2753211/76‑1 (MQ=25) tttGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGc < 1:2227012/76‑1 (MQ=25) ttGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTccc > 2:28955/1‑76 (MQ=25) ttGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc < 1:3355120/76‑1 (MQ=25) ttGCACGAAATCGGAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc < 1:2095496/76‑1 (MQ=25) tGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCg < 1:1109822/76‑1 (MQ=25) aCGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag < 1:432183/76‑1 (MQ=25) aCGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag < 1:1355778/76‑1 (MQ=25) cGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga < 1:1101043/76‑1 (MQ=25) cGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga < 2:3277835/76‑1 (MQ=25) cGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGCGCCGgaga > 2:4846197/1‑76 (MQ=25) gAAATCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGaa < 2:3169628/76‑1 (MQ=25) gAAATCGTAGGGCAGAGAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGaa < 2:922704/76‑1 (MQ=25) gAAATCGGAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGaa < 2:1060853/76‑1 (MQ=25) aaaTCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat < 2:2171669/76‑1 (MQ=25) aaaTCGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat < 1:3322797/76‑1 (MQ=25) aaTCGGAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata < 2:4156898/76‑1 (MQ=25) cGTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCNGGAGAATAtct > 2:4150730/1‑76 (MQ=25) gTAGGGCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtctc > 1:2758650/1‑76 (MQ=37) gggggAGATAAGGGGTTCGCG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCg < 2:527055/75‑1 (MQ=37) gggCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCgg < 1:2501034/76‑1 (MQ=37) ggCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc > 1:4543958/1‑76 (MQ=37) gCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa < 1:4563627/76‑1 (MQ=37) gCAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa > 1:3068975/1‑76 (MQ=37) cAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAt > 1:4082192/1‑76 (MQ=37) cAGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAt > 1:4076746/1‑76 (MQ=37) aGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtt > 1:4636547/1‑76 (MQ=37) aGATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtt > 2:1246484/1‑76 (MQ=37) gATAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttt < 2:1351950/76‑1 (MQ=38) tAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttttt > 1:4358422/1‑76 (MQ=38) tAAGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttttt < 1:701616/76‑1 (MQ=38) aaGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTa > 1:1222007/1‑76 (MQ=38) aaGGCGTT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTa < 2:1726284/76‑1 (MQ=38) || GGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA > NC_000913/3537329‑3537468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |