BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,528	T→C	Q8R (CAG→CGG)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,528	0	T	C	100.0%	273.7 / NA	104	Q8R (CAG→CGG)	araD	L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (43/61); total (43/61)

Only 100 of 104 total aligned reads displayed.

ACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATC  >  NC_000913/66456‑66599

aCGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGc                                                                      <  1:1899747/76‑1 (MQ=255)

 cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGcc                                                                     >  2:3900802/1‑75 (MQ=255)

  ggCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGc                                                                    <  1:3252902‑M1/76‑2 (MQ=255)

   gcgcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcc                                                                   <  1:3938644‑M1/76‑3 (MQ=255)

    cgcTGACGTTGCCCCATGTGAGCGTGGCCAGGTTGTGTTTTGGCAGCGCGGTGTTGTTTTCTAATACCCGGCgccc                                                                  <  1:759690/76‑4 (MQ=255)

     gcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg                                                                 <  1:3243914‑M1/76‑5 (MQ=255)

     gcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccg                                                                 <  2:745343‑M1/76‑5 (MQ=255)

      cTGAGGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccga                                                                <  2:1174149‑M1/76‑6 (MQ=255)

      cTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccca                                                                >  2:495532‑M1/1‑71 (MQ=255)

       tGACGTTGCCCCATGTGAGCGTGAGCGTGTTGTGTTTTGGCGGCGCCAGGTTGGCTTCTAATGCCCGGCGcccgag                                                               <  1:4276833‑M1/76‑7 (MQ=255)

       tGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag                                                               <  2:2271937‑M1/76‑7 (MQ=255)

        gACGTTGCGCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgaga                                                              <  1:2495232‑M1/76‑8 (MQ=255)

        gACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagc                                                              >  1:8232‑M1/1‑69 (MQ=255)

        gACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgaga                                                              <  1:3466670‑M1/76‑8 (MQ=255)

           gTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagaccc                                                           <  1:2476932‑M1/76‑11 (MQ=255)

            ttGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgcgacccg                                                          >  2:3211824‑M1/1‑65 (MQ=255)

            ttGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgcgacccc                                                          >  2:691629‑M1/1‑65 (MQ=255)

            ttGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccg                                                          >  2:3838448‑M1/1‑65 (MQ=255)

             tGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgc                                                         >  2:157163‑M1/1‑64 (MQ=255)

             tGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgc                                                         >  1:4016401‑M1/1‑64 (MQ=255)

             tGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagaccccc                                                         >  2:771063‑M1/1‑64 (MQ=255)

              gCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgca                                                        <  2:510682‑M1/76‑14 (MQ=255)

               ccccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCGAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac                                                       <  2:3113256‑M1/76‑15 (MQ=255)

               ccccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac                                                       <  1:1698891‑M1/76‑15 (MQ=255)

               ccccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac                                                       <  1:1700027‑M1/76‑15 (MQ=255)

               ccccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcac                                                       <  2:4143301‑M1/76‑15 (MQ=255)

                cccATGTGAGCGTGCCCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacg                                                      <  2:3320028‑M1/76‑16 (MQ=255)

                cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacg                                                      >  2:3507067‑M1/1‑61 (MQ=255)

                cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacg                                                      <  1:2313396‑M1/76‑16 (MQ=255)

                cccATGTGAGCGTGACCAGGTGGTGTTTTTGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacg                                                      <  1:2318838‑M1/76‑16 (MQ=255)

                 ccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgc                                                     >  2:1797482‑M1/1‑60 (MQ=255)

                  cATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcg                                                    <  2:2184386‑M1/76‑18 (MQ=255)

                   aTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgtgt                                                   <  2:1741497‑M1/76‑19 (MQ=255)

                   aTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgt                                                   <  1:1618166‑M1/76‑19 (MQ=255)

                    tgtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc                                                  >  2:1074848‑M1/1‑57 (MQ=255)

                    tgtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc                                                  <  2:2394519‑M1/76‑20 (MQ=255)

                    tgtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc                                                  <  1:3607176‑M1/76‑20 (MQ=255)

                       gAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgac                                               >  1:4266577‑M1/1‑54 (MQ=255)

                       gAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgac                                               >  1:3377513‑M1/1‑54 (MQ=255)

                         gCGGGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacct                                             <  2:3418485‑M1/76‑25 (MQ=255)

                            tGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgca                                          <  2:1749438‑M1/76‑28 (MQ=255)

                            tGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgca                                          >  2:1958729‑M1/1‑49 (MQ=255)

                               ccAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt                                       <  1:653044‑M1/76‑31 (MQ=255)

                               ccAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt                                       >  1:2260867‑M1/1‑46 (MQ=255)

                               ccAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt                                       <  1:2959238‑M1/76‑31 (MQ=255)

                               ccAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggt                                       <  1:2053116‑M1/76‑31 (MQ=255)

                                 gggtTGGGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtat                                     <  2:4491208‑M1/75‑33 (MQ=255)

                                 aGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtat                                     >  1:3026532‑M1/1‑44 (MQ=255)

                                 aGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtat                                     <  1:4640174‑M1/76‑33 (MQ=255)

                                  ggTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatg                                    <  1:2313934‑M1/76‑34 (MQ=255)

                                  ggTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatg                                    >  1:739868‑M1/1‑43 (MQ=255)

                                   gTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatgg                                   >  1:1267988‑M1/1‑42 (MQ=255)

                                      gtgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggaga                                <  2:1368656‑M1/76‑38 (MQ=255)

                                       tgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaa                               <  1:1010794‑M1/76‑39 (MQ=255)

                                       tgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaa                               <  1:157163‑M1/76‑39 (MQ=255)

                                       tgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaa                               <  1:3565608‑M1/76‑39 (MQ=255)

                                        gtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcagggatggagaaa                              >  1:1844697‑M1/1‑37 (MQ=255)

                                        gtTTTGGCAGCGCCAGGTTGGCATCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaa                              <  2:3107746‑M1/76‑40 (MQ=255)

                                         ttttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaac                             <  1:197660‑M1/76‑41 (MQ=255)

                                          tttGGGAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaca                            <  2:1373430‑M1/76‑42 (MQ=255)

                                          tttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaca                            <  1:1242884‑M1/76‑42 (MQ=255)

                                          tttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaca                            >  1:3915558‑M1/1‑35 (MQ=255)

                                          tgtggCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaca                            <  1:4557720‑M1/74‑42 (MQ=255)

                                           ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagacacag                           >  2:4331001‑M1/1‑34 (MQ=255)

                                           ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaaccg                           >  2:4314002‑M1/1‑34 (MQ=255)

                                           ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacag                           >  1:3275443‑M1/1‑34 (MQ=255)

                                           ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacag                           >  1:3080217‑M1/1‑34 (MQ=255)

                                            tGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacaga                          >  1:1157813‑M1/1‑33 (MQ=255)

                                             ggCAGCGCCAGGTTGGGTTATAATACCCGGCGcgcgagacccgcacgcggcgacctgcaggtatggagaaacagta                         <  2:1250967‑M1/76‑45 (MQ=255)

                                             ggCAGCGCCAGGTTGGCTTCGAATACCCGGCGcccgagacccgcacgcgccgacctgcaggtatggagaaacagta                         >  1:693224‑M1/1‑32 (MQ=255)

                                              gCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtag                        <  2:3973644‑M1/76‑46 (MQ=255)

                                              gCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtag                        >  2:3926000‑M1/1‑31 (MQ=255)

                                               cAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtaga                       >  1:4270585‑M1/1‑30 (MQ=255)

                                                 gcgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacggtagaga                     <  2:3575043‑M1/76‑49 (MQ=255)

                                                  ggccAGGTTGGCTTCTAATGCCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagag                    <  2:3451801‑M1/75‑50 (MQ=255)

                                                  cgcCAGGTTGGCTTCTAATACCCGGCGcccgagccccgcgcgcgtcgacctgcaggtatggagaaacagtagagag                    <  2:2388031‑M1/76‑50 (MQ=255)

                                                   gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtcgagagg                   >  2:122560‑M1/1‑26 (MQ=255)

                                                   gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt                   <  2:2092975‑M1/76‑51 (MQ=255)

                                                   gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt                   <  1:4456828‑M1/76‑51 (MQ=255)

                                                   gcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagt                   >  1:882986‑M1/1‑26 (MQ=255)

                                                    ccAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagtt                  <  2:4270585‑M1/76‑52 (MQ=255)

                                                      aGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgc                >  1:3811544‑M1/1‑23 (MQ=255)

                                                      aGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgc                <  1:1186057‑M1/76‑54 (MQ=255)

                                                      aGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagaggtgc                >  1:1137427‑M1/1‑23 (MQ=255)

                                                       ggTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggcgaaacagtagagaggtgcg               >  2:943676‑M1/1‑22 (MQ=255)

                                                         ttGGCTTCTAATCCCCGGCGcccgagccccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgat             <  1:1813423‑M1/76‑57 (MQ=255)

                                                          tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata            >  1:558185‑M1/1‑19 (MQ=255)

                                                          tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata            >  1:1296380‑M1/1‑19 (MQ=255)

                                                           ggCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataa           >  1:3804016‑M1/1‑18 (MQ=255)

                                                           ggCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataa           >  1:219092‑M1/1‑18 (MQ=255)

                                                            gCTTCTAAACCCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaa          <  1:308722‑M1/76‑60 (MQ=255)

                                                             cTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaa         >  1:4073356‑M1/1‑16 (MQ=255)

                                                              ttCTAATACCCGGCGcccgagacccgcacgcgtcgccctgcaggtatggagaaacagtagagagttgcgataaaaa        >  2:4367375‑M1/1‑15 (MQ=255)

                                                              ttCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa        <  1:4558691‑M1/76‑62 (MQ=255)

                                                              ttCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa        <  1:1386952‑M1/76‑62 (MQ=255)

                                                                cTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagc      >  2:4546796‑M1/1‑13 (MQ=255)

                                                                cTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagc      <  1:1274443‑M1/76‑64 (MQ=255)

                                                                 tAATACCCGGGGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcg     <  2:212978‑M1/76‑65 (MQ=255)

                                                                 tAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcg     >  1:3767865‑M1/1‑12 (MQ=255)

                                                                    tACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtca  >  1:4457279‑M1/1‑9 (MQ=255)

ACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATC  >  NC_000913/66456‑66599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`