BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,782	A→G	N2S (AAC→AGC)	oppD →	OppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,782	0	A	G	98.5%	347.3 / NA	135	N2S (AAC→AGC)	oppD	OppD
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (79/54); minor base T (1/1); total (80/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TTGTTTCAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAACGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGATTT  >  NC_000913/1302709‑1302851

 tgtttCAGCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGt                                                                    <  2:1807424/76‑1 (MQ=255)

  gtttCAACTTTATCGGCGATTNCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTa                                                                   >  1:2868737/1‑76 (MQ=255)

   tttCAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTaa                                                                  <  1:514489/76‑1 (MQ=255)

    ttCACCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAAt                                                                 <  1:1283036/76‑1 (MQ=255)

    ttCAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCCTGAGCGTAAt                                                                 >  2:677590/1‑76 (MQ=255)

    tacAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGATCGTAAt                                                                 <  1:4750726/74‑1 (MQ=255)

     tCACCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAAtt                                                                <  1:67596/76‑1 (MQ=255)

       aaCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGa                                                              >  1:1949583/1‑76 (MQ=255)

       aaCTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGa                                                              <  1:69270/76‑1 (MQ=255)

          tttATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAAc                                                           >  2:2261648/1‑76 (MQ=255)

            tATGGGCGATGGCTTGCGTGATGCCCGCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTg                                                         <  2:622158/76‑1 (MQ=255)

            tATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACtc                                                         >  1:317151/1‑75 (MQ=255)

            tATCGGCGATGGCTTGCGTGATGCCCCCCACCCGAAAGATCGTTAACGAGTGCCGCCATGAGCGTAATTCAAACTg                                                         >  2:4154997/1‑76 (MQ=255)

             aTCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGc                                                        <  1:790378/76‑1 (MQ=255)

             aTCGGCGATGGCTTGCGTGATGCCCCCGACCCGAAAGATCGTTAAGGAGTGCAGCCCTGAGCGTAATTGAAACTGc                                                        >  2:3012250/1‑76 (MQ=255)

             aTCGGCGATGGCTTGCGTGATGCCCCCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGc                                                        >  2:2534642/1‑76 (MQ=255)

              tCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCa                                                       <  1:571324/76‑1 (MQ=255)

              gcGGCGATGGCTTGCGCAATCCCCTCGACCCGAAACATCGCTAAGGAGCGCACCAATGAGACAAATTGAAACCGCa                                                       >  2:4799485/2‑76 (MQ=255)

               cGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCaa                                                      <  1:3464004/76‑1 (MQ=255)

                ggCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAAc                                                     >  1:2583215/1‑76 (MQ=255)

                 gCGATGGCTTGCGTGATGCCATCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACGGCAACt                                                    >  1:3595418/1‑76 (MQ=255)

                  cGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCACGAGCGTAATTGAAACTGCAACtg                                                   >  2:677602/1‑76 (MQ=255)

                   gATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgt                                                  >  2:4157332/1‑76 (MQ=255)

                   gATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgt                                                  <  1:3654790/76‑1 (MQ=255)

                    aTGGCTTGCGTGATGCCCTCGACCCGGAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAAGTGCAACtgtg                                                 >  1:1766439/1‑76 (MQ=255)

                    aTGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACtgtg                                                 >  2:910299/1‑76 (MQ=255)

                      ggCTTGCGTGATGCGCTCGGCGCGAGAGATCGTTAAGGAGTGCGGCCATGAGCGTAATTGAAACTGCAACTGTGcc                                               <  1:30950/76‑1 (MQ=255)

                      ggCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGcc                                               <  1:2868385/76‑1 (MQ=255)

                      ggCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGcc                                               <  1:209357/76‑1 (MQ=255)

                       gCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCg                                              >  1:3451357/1‑76 (MQ=255)

                       gCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCg                                              <  1:4663246/76‑1 (MQ=255)

                       gCTGGCGTGATGCCATCGACCCGAAAGAACGTTAAGGAGTGAAGCCATGAGCCTAATTGAAACGGCAACTGTGACg                                              >  2:4201188/1‑76 (MQ=255)

                         ttGGGTGATGCCGTCGACCCTAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGCAACTGCACCTGTGCCGCt                                            <  2:1070074/76‑1 (MQ=255)

                         ttGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCt                                            >  1:3233291/1‑76 (MQ=255)

                         ttGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCt                                            >  1:3084007/1‑76 (MQ=255)

                         ttGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCt                                            >  2:1884697/1‑76 (MQ=255)

                         ttGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCt                                            >  1:3965792/1‑76 (MQ=255)

                            cGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGc                                         <  2:1040864/76‑1 (MQ=255)

                             gTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGca                                        >  2:3003667/1‑76 (MQ=255)

                             gTGATGCCCACGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGcc                                        >  2:1467803/1‑75 (MQ=255)

                              tGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGcac                                       >  2:2118319/1‑76 (MQ=255)

                              tGATGCCCGCGACCCAAAATATCGTTAAGAAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGcac                                       <  1:117259/76‑1 (MQ=255)

                                aTGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaa                                     >  1:104960/1‑76 (MQ=255)

                                aTGCCCACGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGATACTGCAACTGTGCCGCTCGcagac                                     >  1:1000284/1‑73 (MQ=255)

                                 tGCCCTGGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaac                                    <  2:1166034/76‑1 (MQ=255)

                                 tGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGGGCCGCTCGCacaac                                    >  2:2202604/1‑76 (MQ=255)

                                  gCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaaca                                   >  1:149602/1‑76 (MQ=255)

                                  gCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaaca                                   >  1:4146431/1‑76 (MQ=255)

                                  gCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaaca                                   >  1:1808456/1‑76 (MQ=255)

                                  gCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaaca                                   >  2:3775303/1‑76 (MQ=255)

                                   cccTCGACCCGAAAGAGCGTTAAGGAGTGCAGAAATGAGCGTAATTGAAAATGCAACTGTGCCGATCGCacaacaa                                  >  2:4325636/1‑76 (MQ=255)

                                    ccTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaac                                 >  1:4321542/1‑76 (MQ=255)

                                    ccTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaac                                 >  1:4328599/1‑76 (MQ=255)

                                    ccTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCacaacaac                                 >  2:1289188/1‑76 (MQ=255)

                                     cTCGACCCTAAAGATCGTTAATGAGCGCATCCATGATCGTAATCCAAACTGCCACTGTCCCGCTATCacaaccacc                                >  2:728949/1‑75 (MQ=255)

                                      tCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAg                               <  2:3534972/76‑1 (MQ=255)

                                      tCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAg                               <  2:3688689/76‑1 (MQ=255)

                                        gACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACCACAGGc                             >  2:426363/1‑76 (MQ=255)

                                        gACCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGc                             >  1:836263/1‑76 (MQ=255)

                                         aCCCGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCt                            >  2:1869539/1‑76 (MQ=255)

                                          ccggAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCTCTCGCACAACAACAGGCTg                           <  1:3876906/73‑1 (MQ=255)

                                          cccGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTg                           >  1:3732671/1‑76 (MQ=255)

                                           ccGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGa                          <  1:4601490/76‑1 (MQ=255)

                                           ccGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGa                          <  2:1949988/76‑1 (MQ=255)

                                           ccGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGa                          >  1:94062/1‑76 (MQ=255)

                                            cGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGAc                         >  2:3952621/1‑76 (MQ=255)

                                            cGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGAc                         <  2:2651244/76‑1 (MQ=255)

                                            cGAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGAc                         >  2:3543689/1‑76 (MQ=255)

                                             gAAAGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAGCTGCAACTGTGCCGCTCGCACAACAACAGGCTGACg                        >  1:2921018/1‑76 (MQ=255)

                                               aaGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCa                      >  1:4833489/1‑76 (MQ=255)

                                               aaGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCa                      >  1:4150272/1‑76 (MQ=255)

                                               aaGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCa                      >  1:2388081/1‑76 (MQ=255)

                                               aaGATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCa                      <  2:2590735/76‑1 (MQ=255)

                                               aaGATCGTGAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCa                      <  2:2863894/76‑1 (MQ=255)

                                                 gATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCAct                    <  1:2548095/76‑1 (MQ=255)

                                                 gATCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCAct                    <  1:3648345/76‑1 (MQ=255)

                                                  aTCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActg                   >  1:2279023/1‑76 (MQ=255)

                                                  aTCGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActg                   >  2:4578020/1‑76 (MQ=255)

                                                    cGTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgct                 >  1:3926776/1‑76 (MQ=255)

                                                     gTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgctg                <  2:2814916/76‑1 (MQ=255)

                                                     gTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgctg                >  2:1231268/1‑76 (MQ=255)

                                                     gTTAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCActgctg                <  1:333756/76‑1 (MQ=255)

                                                      ttAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGa               <  2:4597221/76‑1 (MQ=255)

                                                       tAAGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACACCCGGCTGACGCACTGCTGaa              >  2:3828326/1‑76 (MQ=255)

                                                        aaGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAAc             <  1:2135531/76‑1 (MQ=255)

                                                        aaGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAAc             <  2:4422659/76‑1 (MQ=255)

                                                        aaGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACCCCCTGCTGAAc             >  2:4765716/1‑76 (MQ=255)

                                                         aGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACg            <  2:2516792/76‑1 (MQ=255)

                                                         aGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACg            <  1:1785889/76‑1 (MQ=255)

                                                         aGGAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACg            >  2:4035546/1‑76 (MQ=255)

                                                          ggAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGt           >  1:3251831/1‑76 (MQ=255)

                                                          ggAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGt           <  1:1231269/76‑1 (MQ=255)

                                                          ggAGGGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGt           <  2:3374040/76‑1 (MQ=255)

                                                           gAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTg          <  1:679415/76‑1 (MQ=255)

                                                           gAGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTg          >  2:3024115/1‑76 (MQ=255)

                                                            aGTGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCAGAACGTGa         >  2:3607875/1‑76 (MQ=255)

                                                              tGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGaaa       >  1:2280782/1‑76 (MQ=255)

                                                              tGCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGaaa       >  2:1051581/1‑76 (MQ=255)

                                                               gCAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTAAACGTGAAAg      >  1:1889335/1‑76 (MQ=255)

                                                                cAGCCATGAGCGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGa     >  2:547904/1‑76 (MQ=255)

TTGTTTCAACTTTATCGGCGATGGCTTGCGTGATGCCCTCGACCCGAAAGATCGTTAAGGAGTGCAGCCATGAACGTAATTGAAACTGCAACTGTGCCGCTCGCACAACAACAGGCTGACGCACTGCTGAACGTGAAAGATTT  >  NC_000913/1302709‑1302851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`