| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,978,702 | C→T | S68F (TCC→TTC) | yifK → | putative amino acid/amine transport protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,978,702 | 0 | C | T | 99.3% | 426.3 / NA | 139 | S68F (TCC→TTC) | yifK | putative amino acid/amine transport protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (76/62); minor base G (0/1); total (76/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
CCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCA > NC_000913/3978630‑3978773 | ccGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCt > 2:1148561/1‑76 (MQ=255) cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc < 1:3805721/76‑1 (MQ=255) cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc > 1:535834/1‑76 (MQ=255) cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc < 2:1663799/76‑1 (MQ=255) cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc > 1:287205/1‑76 (MQ=255) cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc > 2:3661988/1‑76 (MQ=255) cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc < 2:4550859/76‑1 (MQ=255) gtgttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg < 2:1972590/73‑1 (MQ=255) gTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg > 2:2797825/1‑76 (MQ=255) gTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg < 2:2049146/76‑1 (MQ=255) aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa < 2:4294674/76‑1 (MQ=255) aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa < 1:4315121/76‑1 (MQ=255) aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa < 1:126951/76‑1 (MQ=255) aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa < 1:468398/76‑1 (MQ=255) ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGCAATGTTGTTCCTCGAac > 2:4082635/1‑76 (MQ=255) tgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc > 1:1870606/1‑76 (MQ=255) tgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc < 1:4085678/76‑1 (MQ=255) tgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggt < 2:3019869/76‑1 (MQ=255) tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg < 1:46219/76‑1 (MQ=255) tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg < 1:3379990/76‑1 (MQ=255) atatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccgg > 2:4193222/1‑76 (MQ=255) atatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGCTGTTCCTCGAaccggttaccgg > 1:4189548/1‑76 (MQ=255) tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggt < 1:2684167/76‑1 (MQ=255) atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt > 1:931082/1‑76 (MQ=255) atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt < 2:4757346/76‑1 (MQ=255) atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt > 1:1378014/1‑76 (MQ=255) tcatcGCCGGGCTGTTCGTCTTTTTCCTCATGCGTTCAATGGGCGAAATGTTGTTCCCCGAACCGGTTACCGgttc > 2:4837431/1‑76 (MQ=255) tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc < 1:4093709/76‑1 (MQ=255) tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc < 1:4344137/76‑1 (MQ=255) tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt < 2:3455996/76‑1 (MQ=255) cGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc > 1:2991877/1‑76 (MQ=255) cGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc < 1:3508669/76‑1 (MQ=255) cGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc < 1:2964637/76‑1 (MQ=255) cGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc < 1:2322542/76‑1 (MQ=255) gCCTGGCTGTTCGTCTTTTTCATCATTCTTTCAATGCGCGAAATGTCTTTCCTCGAACCGGTTACCGgctccttcc > 2:1457423/1‑75 (MQ=255) gCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGttcgttcg < 2:897634/76‑1 (MQ=255) ccGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc > 2:655691/1‑76 (MQ=255) ccGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc > 1:2889941/1‑76 (MQ=255) cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGcc < 1:394435/76‑1 (MQ=255) cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGcc > 2:1369034/1‑76 (MQ=255) cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGcc > 1:480883/1‑76 (MQ=255) cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGcc > 2:573818/1‑76 (MQ=255) gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg > 2:858835/1‑76 (MQ=255) gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGACCCGGTTACCGGTTCGTTCGCCGtt < 1:3856954/76‑1 (MQ=255) gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt > 1:1847398/1‑76 (MQ=255) tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa > 1:17566/1‑76 (MQ=255) tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa > 2:3365959/1‑76 (MQ=255) gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt > 2:562309/1‑76 (MQ=255) gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt < 1:1004950/76‑1 (MQ=255) ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGGACCGGTTACCGGTTCGTTCGCCGTTTATg > 1:2486845/1‑76 (MQ=255) ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATg < 2:1184850/76‑1 (MQ=255) tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAtcc > 2:343399/1‑74 (MQ=255) tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATATTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc > 1:667117/1‑76 (MQ=255) cGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTCATgcc > 2:568169/1‑75 (MQ=255) gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGATCCGGTTACCGGTTCGTTCGCCGTTTATgcgc < 1:959492/76‑1 (MQ=255) gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc < 1:52881/76‑1 (MQ=255) gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc > 1:1315859/1‑76 (MQ=255) gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc > 2:1548209/1‑76 (MQ=255) tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCCTc > 2:63934/1‑76 (MQ=255) tttttCATCATGCGTTCAAGGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTCCGCCGTTTATGCGCATc > 1:4554883/1‑76 (MQ=255) ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCg > 1:2105202/1‑76 (MQ=255) tcatcaTGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta > 2:937323/1‑76 (MQ=255) catcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat > 2:2409382/1‑76 (MQ=255) catcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat > 2:2387207/1‑76 (MQ=255) atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata > 2:4827884/1‑76 (MQ=255) atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata < 1:1194313/76‑1 (MQ=255) atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata > 2:1856430/1‑76 (MQ=255) tcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatat > 2:1862060/1‑76 (MQ=255) tcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatat > 2:3082615/1‑76 (MQ=255) catGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATg > 2:790746/1‑76 (MQ=255) atGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa > 1:2484446/1‑76 (MQ=255) atGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa < 2:3798995/76‑1 (MQ=255) tGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg < 2:4128354/76‑1 (MQ=255) tGCGCTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatatcac > 2:2696727/1‑73 (MQ=255) cGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc > 1:3627105/1‑76 (MQ=255) cGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc < 2:2284488/76‑1 (MQ=255) cGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc < 2:4420369/76‑1 (MQ=255) gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc < 1:4826310/76‑1 (MQ=255) gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc > 2:391209/1‑76 (MQ=255) gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc < 1:100192/76‑1 (MQ=255) tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt < 1:3166734/76‑1 (MQ=255) cAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATTGTTATATGAGCCCGtt < 1:1470350/76‑1 (MQ=255) cAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGtt < 1:4543107/76‑1 (MQ=255) aaTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGTGCCCGTTc > 2:84527/1‑76 (MQ=255) aaTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTc > 1:1944460/1‑76 (MQ=255) tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt > 1:522409/1‑76 (MQ=255) ggCGAAAGGTTGTGCCTCGAACCGGTGACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTg < 2:3935111/76‑1 (MQ=255) gCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg < 1:2849370/76‑1 (MQ=255) gCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg < 1:4771758/76‑1 (MQ=255) gCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTgg < 2:1066334/76‑1 (MQ=255) gAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt > 1:103889/1‑76 (MQ=255) aaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAt < 2:522239/76‑1 (MQ=255) aTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtc > 2:1377243/1‑76 (MQ=255) tgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct > 1:3282435/1‑76 (MQ=255) gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtctc < 1:2472870/76‑1 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa > 1:1334592/1‑76 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa > 2:3640558/1‑76 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa < 2:1496545/76‑1 (MQ=255) ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa < 1:663689/76‑1 (MQ=255) ttgtTCCACGAACCTGTTACCGGTCCGTTCGCCGTTTATGCGCCTCTTTATATGAGCCCATTCTTCGTCCATCTCa < 1:2536734/76‑1 (MQ=255) | CCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCA > NC_000913/3978630‑3978773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |