BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,626	T→C	E180E (GAA→GAG)	rspA ←	starvation sensing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,626	0	T	C	100.0%	283.1 / NA	104	E180E (GAA→GAG)	rspA	starvation sensing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (55/49); total (55/49)

Only 100 of 108 total aligned reads displayed.

CAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGT  >  NC_000913/1652556‑1652697

cAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                    >  1:2500183/1‑76 (MQ=255)

  aaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                  <  2:1442221/76‑1 (MQ=255)

  aaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                  <  2:281887/76‑1 (MQ=255)

   aCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCgg                                                                 >  1:4658295/1‑76 (MQ=255)

   aCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCgg                                                                 <  2:4244429/76‑1 (MQ=255)

    cTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                <  2:2388715/76‑1 (MQ=255)

     ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTGTCCGTCGACCACAGCTGCTGTTACGGcc                                                               >  1:1199902/1‑76 (MQ=255)

     ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGcc                                                               <  2:1210506/76‑1 (MQ=255)

      tGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCa                                                              <  2:1913490/76‑1 (MQ=255)

         tACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACtc                                                           >  2:2667704/1‑75 (MQ=255)

         tACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                           >  2:2717222/1‑76 (MQ=255)

          aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGt                                                          >  1:4305566/1‑76 (MQ=255)

          aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGt                                                          >  2:4705715/1‑76 (MQ=255)

           cGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGGCCACAGCTGCTCTTCCGGCCACTGTc                                                         <  2:3943393/76‑1 (MQ=255)

            gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTcc                                                        >  1:2665496/1‑76 (MQ=255)

             tACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCt                                                       <  1:543250/76‑1 (MQ=255)

              aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                      <  2:3433936/76‑1 (MQ=255)

                cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                    <  1:1058348/76‑1 (MQ=255)

                cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                    <  1:531512/76‑1 (MQ=255)

                 gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                   >  2:829742/1‑76 (MQ=255)

                    tCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTg                                                <  1:2548444/76‑1 (MQ=255)

                      aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCg                                              >  2:627093/1‑76 (MQ=255)

                       aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCgg                                             >  2:674860/1‑76 (MQ=255)

                       aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCgg                                             <  1:4861779/76‑1 (MQ=255)

                         cAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGt                                           >  2:558943/1‑76 (MQ=255)

                            tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCa                                        <  2:2665853/76‑1 (MQ=255)

                            tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCa                                        >  1:3128918/1‑76 (MQ=255)

                            tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCa                                        <  2:4401679/76‑1 (MQ=255)

                            tttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCa                                        >  1:4363983/1‑76 (MQ=255)

                              tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCCTa                                      >  2:3595689/1‑76 (MQ=255)

                              tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATa                                      >  1:3618002/1‑76 (MQ=255)

                              tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATa                                      <  2:2768069/76‑1 (MQ=255)

                              tCGGCATGAAAGCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATa                                      <  2:3225358/76‑1 (MQ=255)

                               cGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                     <  2:1778755/76‑1 (MQ=255)

                                ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAg                                    <  1:1973658/76‑1 (MQ=255)

                                 gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGc                                   >  2:4096810/1‑76 (MQ=255)

                                  cATGAAATCGAGGTATTTCTCCGTCGCCCGCAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                  <  1:4677669/76‑1 (MQ=255)

                                   aTGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCa                                 <  1:814484/76‑1 (MQ=255)

                                    tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAg                                >  2:1319851/1‑76 (MQ=255)

                                       aaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                             <  2:2644130/76‑1 (MQ=255)

                                       aaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                             <  2:3713626/76‑1 (MQ=255)

                                        aTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                            <  2:1923104/76‑1 (MQ=255)

                                         tCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                           <  2:4421732/76‑1 (MQ=255)

                                         tCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                           >  2:2734945/1‑76 (MQ=255)

                                         tCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                           <  2:4442561/76‑1 (MQ=255)

                                          cGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTCTTTGCGGGTTCATAACCCCGaccttc                          >  2:330279/1‑75 (MQ=255)

                                           gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                         >  2:1494188/1‑76 (MQ=255)

                                           gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                         <  1:1863462/76‑1 (MQ=255)

                                           gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                         >  1:452494/1‑76 (MQ=255)

                                            aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttac                        >  1:2725376/1‑76 (MQ=255)

                                             ggTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacc                       >  2:3269300/1‑76 (MQ=255)

                                               tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                     <  1:2625400/76‑1 (MQ=255)

                                               tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                     <  1:1383079/76‑1 (MQ=255)

                                               tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                     <  1:3713294/76‑1 (MQ=255)

                                               tATTTCTCCGGCGACCACAGCTGCTCTGCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                     <  1:1411523/76‑1 (MQ=255)

                                               tATTGCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                     <  1:627093/76‑1 (MQ=255)

                                                aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttaccttt                    >  1:3847972/1‑76 (MQ=255)

                                                aTTTCTCCGTCGACCACAGCTGCGCTTCCGGCCACTGTCCTTTGGTTGCGTGTTCATAAGCCAGacctttaccttt                    <  1:1045446/76‑1 (MQ=255)

                                                 tttCTCTGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                   >  2:1653795/1‑76 (MQ=255)

                                                 tttCTCCGTCGACCGCAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                   <  2:4537140/76‑1 (MQ=255)

                                                 tttCGCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                   <  1:1651036/76‑1 (MQ=255)

                                                  ttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCg                  >  1:2208023/1‑76 (MQ=255)

                                                  ttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCg                  <  2:4303644/76‑1 (MQ=255)

                                                   tctcCGTCGACCACAGCTGCTCTTCCGGCCCCTGTCCTTTGGTTGCGGGTCCACAACCCAGCCCTTTACCtctccc                 >  2:302766/1‑74 (MQ=255)

                                                   tctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCgc                 >  2:3401871/1‑75 (MQ=255)

                                                    ctcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAc                <  1:3303733/76‑1 (MQ=255)

                                                     tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa               >  2:1522883/1‑76 (MQ=255)

                                                     tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa               >  2:3850298/1‑76 (MQ=255)

                                                      ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt              <  1:2488219/76‑1 (MQ=255)

                                                      ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt              <  1:4096810/76‑1 (MQ=255)

                                                      ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt              >  1:190564/1‑76 (MQ=255)

                                                       cGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATg             >  1:1830562/1‑76 (MQ=255)

                                                        gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc            >  1:2573224/1‑76 (MQ=255)

                                                        gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc            >  2:2776445/1‑76 (MQ=255)

                                                        gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGc            >  2:2923549/1‑76 (MQ=255)

                                                         tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc           <  2:863209/76‑1 (MQ=255)

                                                         tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc           >  1:748804/1‑76 (MQ=255)

                                                         tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc           >  2:3822162/1‑76 (MQ=255)

                                                          cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCg          <  1:3434339/76‑1 (MQ=255)

                                                          cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCg          >  1:3674606/1‑76 (MQ=255)

                                                           gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGCCCTTTACCTTTAGACCTGCCGt         >  2:243037/1‑76 (MQ=255)

                                                           gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt         >  1:1607190/1‑76 (MQ=255)

                                                           gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt         <  1:2717222/76‑1 (MQ=255)

                                                           gACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGt         >  1:2425044/1‑76 (MQ=255)

                                                            gccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa        <  1:1922989/75‑1 (MQ=255)

                                                            aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCCTAAGCCAGACCTTTACCTTTCGACATCCCgca        >  2:1626303/1‑74 (MQ=255)

                                                             ccccaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg       <  1:4515709/73‑1 (MQ=255)

                                                             ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg       >  1:4271988/1‑76 (MQ=255)

                                                             ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg       >  1:3824058/1‑76 (MQ=255)

                                                             ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg       <  2:1487512/76‑1 (MQ=255)

                                                               acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt     <  2:2537273/76‑1 (MQ=255)

                                                               acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGCAggt     >  2:4436168/1‑76 (MQ=255)

                                                                caGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCCGACCTTTACCTTTCGACATGCCGTAggcg    >  2:1727018/1‑74 (MQ=255)

                                                                caGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTAGACATGACGTAggtg    >  1:4076780/1‑76 (MQ=255)

                                                                 aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg   >  1:4787605/1‑76 (MQ=255)

                                                                 aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg   <  1:3580780/76‑1 (MQ=255)

                                                                 aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg   >  2:3642767/1‑76 (MQ=255)

                                                                  gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt  <  1:1326177/76‑1 (MQ=255)

                                                                  gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt  <  1:1653796/76‑1 (MQ=255)

                                                                  gctgctCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt  <  1:13638/76‑1 (MQ=255)

CAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGT  >  NC_000913/1652556‑1652697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`