BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,743	C→T	P101L (CCC→CTC)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,743	0	C	T	97.7%	398.7 / ‑4.9	133	P101L (CCC→CTC)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (62/68); minor base G (1/2); total (63/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA  >  NC_000913/4016671‑4016815

tGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaa                                                                       <  2:1624319/76‑1 (MQ=255)

tGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaa                                                                       <  2:2659712/76‑1 (MQ=255)

 gACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaca                                                                      >  2:2992186/1‑74 (MQ=255)

 gACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaaa                                                                      <  2:1243939/76‑1 (MQ=255)

 gACAGCGAAATTCTCCCGCCTGGTCGGAGAAACGGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCaaa                                                                      <  2:1267039/76‑1 (MQ=255)

   cAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAt                                                                    <  2:2374372/76‑1 (MQ=255)

   cAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAt                                                                    >  1:281543/1‑76 (MQ=255)

    aGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATg                                                                   >  2:873816/1‑76 (MQ=255)

     gCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGACTCCATGCTCAAAATgg                                                                  <  1:1788681/76‑1 (MQ=255)

      cGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATggg                                                                 >  2:3556769/1‑76 (MQ=255)

      cGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATggg                                                                 <  2:1995554/76‑1 (MQ=255)

       gAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGCTATCAATGAATCCATGCTCAAAATggtc                                                                <  1:2379529/76‑3 (MQ=255)

       gAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGc                                                                >  2:3641790/1‑76 (MQ=255)

        aaaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                               >  2:3646858/1‑76 (MQ=255)

        aaaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                               <  1:642830/76‑1 (MQ=255)

        aaaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                               <  1:62806/76‑1 (MQ=255)

          aTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgc                                                             <  1:4206095/76‑1 (MQ=255)

              tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgaga                                                         <  1:2303586/76‑1 (MQ=255)

               cccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGaa                                                        >  2:2039844/1‑76 (MQ=255)

                ccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAg                                                       >  1:137065/1‑76 (MQ=255)

                 cGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                      >  2:2395506/1‑76 (MQ=255)

                  gCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCt                                                     <  2:1548879/76‑1 (MQ=255)

                  gCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCt                                                     <  2:3611174/76‑1 (MQ=255)

                   ccTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTg                                                    >  2:1954120/1‑76 (MQ=255)

                    cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGc                                                   <  2:2244150/76‑1 (MQ=255)

                    cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGc                                                   >  2:83316/1‑76 (MQ=255)

                     ttgTCGGAGAAGCTGGCAAAGTGGTCCTTGCTGATATCAATGATTCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                  <  2:1655932/74‑1 (MQ=255)

                     tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                  <  2:4096360/76‑1 (MQ=255)

                     tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                  >  2:878303/1‑76 (MQ=255)

                     tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                  >  1:1577955/1‑76 (MQ=255)

                     tGGTCGGAGAAACGGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                  <  2:1628027/76‑1 (MQ=255)

                        tCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                               <  1:4217778/76‑1 (MQ=255)

                        tCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                               >  1:2789236/1‑76 (MQ=255)

                         cGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAat                                              <  2:2698385/76‑1 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTCata                                             >  2:2321945/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                             >  2:2686724/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                             >  1:2773011/1‑76 (MQ=255)

                          ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAata                                             >  2:1136547/1‑76 (MQ=255)

                            agaAGCTTGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAAGATc                                           <  2:2127869/76‑1 (MQ=255)

                              aaaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                         >  1:1330128/1‑76 (MQ=255)

                               aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                        <  1:2773709/76‑1 (MQ=255)

                               aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                        <  2:3578916/76‑1 (MQ=255)

                                aCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                       >  2:2094500/1‑76 (MQ=255)

                                 cTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtgt                                      >  2:1911941/1‑76 (MQ=255)

                                 cTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGGAATATCGgtgt                                      >  2:685879/1‑76 (MQ=255)

                                  tGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGtgtg                                     >  2:1230638/1‑76 (MQ=255)

                                   ggCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGa                                    <  2:2687443/76‑1 (MQ=255)

                                     gaaaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                  <  2:3157/75‑1 (MQ=255)

                                     gaaaGTGGTCCGTGCTGATATAAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                  <  2:1119860/75‑1 (MQ=255)

                                     cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                  <  1:1995020/76‑1 (MQ=255)

                                      aaaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                                 >  1:2707239/1‑76 (MQ=255)

                                       aaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCGGCGTAATATCGGTGTGTTTgg                                >  2:66063/1‑76 (MQ=255)

                                        aGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGc                               >  1:1638670/1‑76 (MQ=255)

                                         gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCa                              >  2:423520/1‑76 (MQ=255)

                                         gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCa                              >  2:4143601/1‑76 (MQ=255)

                                          tGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCac                             >  2:456604/1‑75 (MQ=255)

                                          tGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCaa                             >  1:907183/1‑76 (MQ=255)

                                          tGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCaa                             <  1:2683204/76‑1 (MQ=255)

                                           ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                            >  1:4632881/1‑76 (MQ=255)

                                           ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                            >  1:219466/1‑76 (MQ=255)

                                            gTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACg                           >  1:4686495/1‑76 (MQ=255)

                                             tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGt                          >  1:4355943/1‑76 (MQ=255)

                                             tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGt                          >  2:2364419/1‑76 (MQ=255)

                                                ttGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                       >  1:2693872/1‑76 (MQ=255)

                                                ttGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                       >  2:465793/1‑76 (MQ=255)

                                                 tGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAg                      >  1:3516073/1‑76 (MQ=255)

                                                 tGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAg                      <  1:873817/76‑1 (MQ=255)

                                                  gCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGt                     >  2:3389988/1‑76 (MQ=255)

                                                  gCTGATATCAATGAATCAATGCTAAAAATGGGCCGAGCGAAGCGGCGTAATATAGGTGTGATTGGCAACCTTGAgg                     >  2:1824047/1‑75 (MQ=255)

                                                   cTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTa                    <  2:611750/76‑1 (MQ=255)

                                                   cTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTa                    >  1:3243785/1‑76 (MQ=255)

                                                    tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAt                   <  2:4340298/76‑1 (MQ=255)

                                                      atatCAATGAATCCATGCGCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGt                 <  2:3835622/76‑1 (MQ=255)

                                                          cAATGAATCCATGCTCACAATGGGCCGCGAGACGCTGCGTAATATCGGAGTGATTGGCAACGGTGAGTATGGTCAg             >  1:3539129/1‑76 (MQ=255)

                                                          cAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg             <  2:1747098/76‑1 (MQ=255)

                                                          cAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg             <  1:1929634/76‑1 (MQ=255)

                                                          cAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAg             <  1:4816699/76‑1 (MQ=255)

                                                           aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAgg            <  1:2504831/76‑1 (MQ=255)

                                                           aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAgg            >  2:4243828/1‑76 (MQ=255)

                                                           aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAgg            <  1:43772/76‑1 (MQ=255)

                                                           aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGGAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAgg            >  1:554832/1‑76 (MQ=255)

                                                             tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCg          <  1:2703093/76‑1 (MQ=255)

                                                             tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCg          >  2:3499836/1‑76 (MQ=255)

                                                             tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCg          >  2:1992766/1‑76 (MQ=255)

                                                             tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCg          <  2:2490053/76‑1 (MQ=255)

                                                              gAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGa         >  1:3645738/1‑76 (MQ=255)

                                                              gAATCCATGCGCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGa         <  2:1679325/76‑1 (MQ=255)

                                                               aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa        <  2:3986658/76‑1 (MQ=255)

                                                               aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGaa        >  1:3561719/1‑76 (MQ=255)

                                                                aTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAc       <  1:1640926/76‑1 (MQ=255)

                                                                 tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACg      <  2:1101192/76‑1 (MQ=255)

                                                                 tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACg      <  1:867139/76‑1 (MQ=255)

                                                                  ccATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGc     <  2:2131224/76‑1 (MQ=255)

                                                                  ccATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGc     <  1:4452917/76‑1 (MQ=255)

                                                                   cATGCTCAAAATGGGCCGCGAGACGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCt    >  2:2208257/1‑76 (MQ=255)

                                                                   cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCt    <  1:1286749/76‑1 (MQ=255)

                                                                    aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTg   >  2:2624794/1‑76 (MQ=255)

                                                                    aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTg   >  1:1331676/1‑76 (MQ=255)

                                                                     tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa  <  1:4016816/76‑1 (MQ=255)

                                                                     tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGa  <  1:2740161/76‑1 (MQ=255)

TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA  >  NC_000913/4016671‑4016815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`