| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,091,351 | C→T | G168D (GGC→GAC) | rhaD ← | rhamnulose‑phosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,091,351 | 0 | C | T | 98.6% | 421.5 / NA | 142 | G168D (GGC→GAC) | rhaD | rhamnulose‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (72/68); minor base G (0/2); total (72/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 145 total aligned reads displayed.
TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG > NC_000913/4091278‑4091423 | ttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt > 2:4348492/1‑76 (MQ=255) tggTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCtt < 2:3391190/74‑1 (MQ=255) tCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCtt < 2:945901/76‑1 (MQ=255) cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc < 2:2890189/76‑1 (MQ=255) gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc < 1:2293327/76‑1 (MQ=255) gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCCGTCTTcc > 2:1534400/1‑76 (MQ=255) tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc > 2:4095283/1‑76 (MQ=255) tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc > 1:1626939/1‑76 (MQ=255) ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcccc > 2:2994348/1‑75 (MQ=255) ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCACACACTCGGTGCTGTCTTCCca > 2:4857760/1‑76 (MQ=255) gTGCCGGGCTCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCATACACTCGGTGCTGTCTTCCcaca < 1:2105153/76‑1 (MQ=255) gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca > 2:3627762/1‑76 (MQ=255) gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca > 1:1991956/1‑76 (MQ=255) ggCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAg < 1:1215191/75‑1 (MQ=255) gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt > 2:2021334/1‑76 (MQ=255) ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt > 2:3293006/1‑76 (MQ=255) ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt > 1:84144/1‑76 (MQ=255) gggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg < 1:1973380/76‑1 (MQ=255) gggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg > 1:637728/1‑76 (MQ=255) cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa < 1:2836913/76‑1 (MQ=255) cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa > 2:1642387/1‑76 (MQ=255) cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa < 1:1204954/76‑1 (MQ=255) aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACCGTTGGCGAg > 1:4696080/1‑76 (MQ=255) aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg > 1:3705083/1‑76 (MQ=255) ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt < 1:1655253/76‑1 (MQ=255) cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTg > 2:1668905/1‑76 (MQ=255) aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa < 2:3881104/76‑1 (MQ=255) aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa < 1:3221547/76‑1 (MQ=255) tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa < 2:1991955/76‑1 (MQ=255) tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa < 1:706869/76‑1 (MQ=255) ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg < 1:1878850/76‑1 (MQ=255) cgcGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa < 2:3186266/74‑1 (MQ=255) cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa > 1:4820277/1‑76 (MQ=255) cACGGCAAAATGCCAACGCCATCCGGGAACACCACCAGACACTCGCTGCTGTCTTCCCACACACGGCGACTGCAGa > 2:4400008/1‑76 (MQ=255) aCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGCTGCTGTCTTCCCACAGTTGGCGAGTGAAGAc > 1:2102277/1‑76 (MQ=255) ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcg > 1:1385783/1‑76 (MQ=255) gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc < 1:2327308/76‑1 (MQ=255) gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc > 2:2651594/1‑76 (MQ=255) gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc < 1:3953892/76‑1 (MQ=255) gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc < 1:4730776/76‑1 (MQ=255) cAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGACGACcgcg > 2:1172791/1‑76 (MQ=255) cAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg < 1:920438/76‑1 (MQ=255) cAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg < 1:186881/76‑1 (MQ=255) aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGGGCTGTCTGCCCACCGTCGGCGAGTGAAGACCGCgg > 2:423446/1‑76 (MQ=255) aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt < 2:3872435/76‑1 (MQ=255) aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg > 1:2379949/1‑76 (MQ=255) gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg < 1:2597507/76‑1 (MQ=255) gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg > 2:3283792/1‑76 (MQ=255) gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg < 1:4323519/76‑1 (MQ=255) ccAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt < 1:3214350/76‑1 (MQ=255) ccAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTCAAGACCGCGGTGTCGt > 2:3753598/1‑76 (MQ=255) cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt > 1:4318970/1‑76 (MQ=255) aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt > 2:4021957/1‑76 (MQ=255) aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt < 2:4300096/76‑1 (MQ=255) aCGCCATCGGGGAATACCACCAGACTCTCGGTGCTGTCTTCCCACAGTTGGCGATTGAAGAGCGCGGTGTCGtttt < 1:813468/76‑1 (MQ=255) cGCCATCCGGGAATACCACCAGACACTCGGCGCTGTCTTCCCCCAGTCGGCGAGTGAAGACCGCGGGGGCGTTTTc > 2:42485/1‑76 (MQ=255) gCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCa < 2:2818572/76‑1 (MQ=255) gCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCa > 2:3143439/1‑76 (MQ=255) ccATCCCGGGATACCACCAGACACTCACCGCTGTCTTTCCACAGTTGGCGCGTGGAGACCGCGGTGTCGTATTcga > 2:1141307/1‑74 (MQ=255) ctCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt < 1:3372781/75‑1 (MQ=255) aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCACGt > 2:254983/1‑76 (MQ=255) aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt > 1:1389779/1‑76 (MQ=255) aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt > 1:2527818/1‑76 (MQ=255) aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt > 1:2152552/1‑76 (MQ=255) aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt > 1:3217672/1‑76 (MQ=255) aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt < 1:3854169/76‑1 (MQ=255) ccGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAc < 2:1844995/76‑1 (MQ=255) gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAt > 2:2581700/1‑76 (MQ=255) ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa < 1:2885207/76‑1 (MQ=255) ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGCGAAGCCCGCGGTGGCGTTTTCAAGTCCATa > 2:4592732/1‑76 (MQ=255) aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg > 2:4530791/1‑76 (MQ=255) aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg < 1:42086/76‑1 (MQ=255) aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAgg > 1:3962105/1‑76 (MQ=255) aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAggg > 2:3189002/1‑75 (MQ=255) tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTg < 1:3283792/76‑1 (MQ=255) caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg < 1:4520325/76‑1 (MQ=255) caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg < 1:3779249/76‑1 (MQ=255) caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg < 1:1445212/76‑1 (MQ=255) caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg > 1:481080/1‑76 (MQ=255) caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCCCGGTGTCGTTTTCCAGTACATAGGTGCgg > 2:603215/1‑76 (MQ=255) cccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg < 2:1126836/75‑1 (MQ=255) accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg < 1:3293006/76‑1 (MQ=255) accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg > 2:936525/1‑76 (MQ=255) accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg < 1:3778951/76‑1 (MQ=255) cggACACGCGGTGGTGGCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg < 2:280703/74‑1 (MQ=255) aGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGa < 1:1098038/76‑1 (MQ=255) acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg > 1:1753833/1‑73 (MQ=255) acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg < 2:1753832/73‑1 (MQ=255) acacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc < 1:4775342/76‑1 (MQ=255) acacTCGGTGCCGGCCACCCGCTGTCCGCGATTGAATACCGCGGTGTAGTTTTCAAATTCATAGGTGAGGGCGATc < 1:162522/76‑1 (MQ=255) cacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCa > 1:3482478/1‑76 (MQ=255) cacTCAGTCCTGTCTGCCCACAGTTGGCGAGTCAAGCCCGCGGTGGCGTTCTCAAGAACATAGGTGCGGGCGATCa > 2:272957/1‑76 (MQ=255) cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg < 2:3404069/76‑1 (MQ=255) tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt < 1:3313709/76‑1 (MQ=255) ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTCCATAGGGGAGGGCGATCCGGTtg > 2:3367510/1‑76 (MQ=255) ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg > 1:3157474/1‑76 (MQ=255) ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg < 1:1638806/76‑1 (MQ=255) ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg < 1:2163521/76‑1 (MQ=255) gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtg < 1:1543553/76‑1 (MQ=255) gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGGACATAGGTGAGGGCGATCGGGTtgggg > 1:298341/1‑74 (MQ=255) | TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG > NC_000913/4091278‑4091423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |