| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,316,203 | T→A | Q47L (CAG→CTG) | phnK ← | ATP‑binding component of phosphonate transport |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,316,203 | 0 | T | A | 98.6% | 413.2 / ‑5.6 | 142 | Q47L (CAG→CTG) | phnK | ATP‑binding component of phosphonate transport |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (62/77); minor base C (1/1); total (63/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
Only 100 of 147 total aligned reads displayed.
GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT > NC_000913/4316131‑4316275 | cATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGt < 2:3282750/76‑1 (MQ=255) aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTg < 1:3094107/76‑1 (MQ=255) aTACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCCGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTg > 1:2974279/1‑76 (MQ=255) tACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTgg < 1:4225083/76‑1 (MQ=255) aCAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGt < 1:358671/76‑1 (MQ=255) aCAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGt > 2:741173/1‑76 (MQ=255) aCAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGt > 2:1026017/1‑76 (MQ=255) aCAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGt < 1:3697947/76‑1 (MQ=255) cAGCGAACGGTTCTCGTAGGGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTc < 1:4397895/76‑1 (MQ=255) gCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGAGCGCGGAGATCG‑ACTTCAGCAGCGCGGTCtc > 2:2338560/1‑75 (MQ=255) gCGAACGGTTCTCGTAGTGAAGTTCCCCCTGCTGCGGCGTCAGGCTCGCGGAGATCG‑ACTTCAGCAGCGTGGTCtt < 2:2291424/76‑1 (MQ=255) gAACGGTTCTGGTAGTGAATTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTcc < 2:935591/76‑1 (MQ=255) gAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTcc < 2:275701/76‑1 (MQ=255) aCGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCg < 2:1808070/76‑1 (MQ=255) cGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCTCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCgg < 2:2120096/76‑1 (MQ=255) ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGa > 1:4096880/1‑76 (MQ=255) ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGa > 2:198695/1‑76 (MQ=255) ggTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGa > 2:499581/1‑76 (MQ=255) ggTTCGCGTGGTGAATTTCCCCCGGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGa < 2:4651543/76‑1 (MQ=255) gTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAg > 1:3262328/1‑76 (MQ=255) gTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAg > 2:2887668/1‑76 (MQ=255) gTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAg > 2:3027522/1‑76 (MQ=255) ttCTCGTAGTGTGTTGCCCACTGCTGCGGCGTCTGGCTCGCGGAGATCG‑ACTTCAGCTTCGTTGTCTTCCCGGAGc < 1:2789188/76‑1 (MQ=255) ttCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCGCAC‑ACCGCCACGCGGTCTTCCCGGAGc > 2:2601652/1‑76 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGcc < 1:1805052/76‑1 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGcc < 1:198695/76‑1 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGcc < 1:2957610/76‑1 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGcc > 2:3292847/1‑76 (MQ=255) tctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGcc < 1:2478406/76‑1 (MQ=255) gctcGTAGGGAATTTCCCCCTGCGGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGcc < 2:2049159/75‑1 (MQ=255) ctcGTAGTGAATTTCCCCGTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCg < 1:578327/76‑1 (MQ=255) ctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCg < 1:42280/76‑1 (MQ=255) ctcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCg > 1:4469469/1‑76 (MQ=255) tcGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCgg < 2:2145874/76‑1 (MQ=255) cGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa > 2:3641451/1‑76 (MQ=255) cGTAGTGAATTCCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGa < 1:124768/76‑1 (MQ=255) gTAGTGAATTTCCCCCTGCTGCGCCGTCAGCCGCGCGGCGACCG‑ACTTCAGCAGCGTGGTCTCCCCGGACCCggtc > 2:822172/1‑74 (MQ=255) tAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑AATTCAGCAGCGTGGGCTTCCCGGAGCCGGAtt > 2:3731422/1‑76 (MQ=255) gaGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGCCTTCCCGGAGCCGGAtt < 2:236147/75‑1 (MQ=255) aGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTc < 1:3913950/76‑1 (MQ=255) gTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcc > 2:4252737/1‑76 (MQ=255) tGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTccc < 2:3524127/76‑1 (MQ=255) gAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTcccc < 1:3554085/76‑1 (MQ=255) tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACac > 2:3598550/1‑75 (MQ=255) tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa > 2:3084015/1‑76 (MQ=255) tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACaa < 2:2522979/76‑1 (MQ=255) tCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCAGATTCCCCGACaa > 1:2741068/1‑76 (MQ=255) cccccTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGCGCCGGACTCCCCGACaac > 2:566086/1‑75 (MQ=255) cccTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGc > 1:917625/1‑76 (MQ=255) ccggctgcGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc < 2:1651250/73‑1 (MQ=255) ccTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGcc < 2:3458368/76‑1 (MQ=255) ctgctgCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGccc > 1:141842/1‑76 (MQ=255) ctgctgCGGCGTCAGGCGCGCGGAGATCG‑ACTGCAGCAGAGTGGGCTTCCCGGAGCCGGACTCCACGGCATGGccc > 2:1676693/1‑76 (MQ=255) tgctgcGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa < 1:3518478/76‑1 (MQ=255) ggctgcGGCGTCAGGCGCGGGGAGATCG‑AGTTCTGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCa < 2:848601/75‑1 (MQ=255) gctgcGGCGGCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAg < 2:467183/76‑1 (MQ=255) tgcGGCGTCAGGCGCGCGGAGATCG‑ACTTCGGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa < 2:679510/76‑1 (MQ=255) tgcGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa > 1:4512244/1‑76 (MQ=255) tgcGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCa > 1:667842/1‑76 (MQ=255) gcggcgTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCAc < 2:2649587/76‑1 (MQ=255) cggcgTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt > 1:3142791/1‑76 (MQ=255) cggcgTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACt > 2:3220695/1‑76 (MQ=255) ggcgTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACtt > 1:4681516/1‑76 (MQ=255) gTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc < 2:4377269/76‑1 (MQ=255) gTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc > 1:54450/1‑76 (MQ=255) gTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc > 1:1706267/1‑76 (MQ=255) gTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc < 2:2409255/76‑1 (MQ=255) gTCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGAGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccc > 1:4197627/1‑76 (MQ=255) tCAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTcccc < 2:3722429/76‑1 (MQ=255) gccggCGCGCGGAGATCG‑GCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGGCAATGCCCAGCACTTcccc < 2:1035005/73‑1 (MQ=255) gaGGCGGGCGGGGGTGG‑ACTTCCGCAGCGGGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc < 2:4448558/75‑1 (MQ=255) ctggcgggcgGAGTTCG‑GCTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc < 1:1078958/74‑1 (MQ=255) cAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc > 2:3599897/1‑76 (MQ=255) cAGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTccccc < 2:1052027/76‑1 (MQ=255) aGGCGCGCGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCg > 2:3069024/1‑76 (MQ=255) gcgggcggAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGc < 2:1559297/76‑1 (MQ=255) gcgcgcGGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGc < 1:3305999/76‑1 (MQ=255) cgcgcgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGcc < 1:3599268/76‑1 (MQ=255) cgcgcgGAGATCG‑ACTTCAGCAGCGTGGGCTTCCCGGAGCAGGATTCCACGACAATGCCCAGCAATTCCCCCGGcc > 2:2750956/1‑76 (MQ=255) gcgcgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa > 1:491520/1‑76 (MQ=255) gcgcgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCa < 2:3857234/76‑1 (MQ=255) cgcgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCAt < 1:3905762/76‑1 (MQ=255) gcgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCCTa > 2:431447/1‑76 (MQ=255) gcgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATa > 1:2341975/1‑76 (MQ=255) cgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa > 2:3532622/1‑76 (MQ=255) cgGAGATCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATaa < 1:951731/76‑1 (MQ=255) gagaTCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAAt > 2:3397917/1‑76 (MQ=255) gaTCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGTATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCa < 1:1009145/76‑1 (MQ=255) aTCG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaa > 2:2282646/1‑76 (MQ=255) cG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa > 2:3940970/1‑76 (MQ=255) cG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa > 2:3909987/1‑76 (MQ=255) cG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa > 1:3239681/1‑76 (MQ=255) cG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa > 1:4814273/1‑76 (MQ=255) cG‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCaaaa < 1:741173/76‑1 (MQ=255) g‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag > 2:4021166/1‑76 (MQ=255) g‑ACTTCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAag > 1:3166141/1‑76 (MQ=255) ctgcagcagCGTGCTGTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAagag < 2:917624/73‑1 (MQ=255) ttCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAgaga < 1:1007435/76‑1 (MQ=255) tCAGCAGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGAc < 1:555612/76‑1 (MQ=255) agcagcGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAt < 1:1293548/76‑1 (MQ=255) | GCATACAGCGAACGGTTCTCGTAGTGAATTTCCCCCTGCTGCGGCGTCAGGCGCGCGGAGATCG‑ACTTCAGCTGCGTGGTCTTCCCGGAGCCGGATTCCCCGACAATGCCCAGCACTTCCCCCGGCCATAAATCAAAAGAGACAT > NC_000913/4316131‑4316275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |