Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,472,408	A→G	intergenic (+117/‑28)	b4251 → / → b4252	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,472,408	0	A	G	100.0%	251.9 / NA	94	intergenic (+117/‑28)	b4251/b4252	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/50);  total (44/50)

TGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAG  >  NC_000913/4472336‑4472480                                                                         |                                                                         tgtgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgt                                                                       <  2:2685426/76‑1 (MQ=255) tgtgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgt                                                                       >  2:3634070/1‑76 (MQ=255)  gtgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgtt                                                                      <  1:1130684/76‑1 (MQ=255)  gtgATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgtt                                                                      <  2:3854020/76‑1 (MQ=255)       aaGACAATAATTCACGCGTTAAGGCGAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGGTGtttt                                                                 >  2:2149063/1‑76 (MQ=255)        aGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAGGCGTTGTTtta                                                                >  1:300619/1‑76 (MQ=255)        aGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTtta                                                                <  1:3327771/76‑1 (MQ=255)         gACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaa                                                               >  2:3675189/1‑76 (MQ=255)         gACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaa                                                               <  1:3028420/76‑1 (MQ=255)           cAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaatt                                                             <  1:2103071/76‑1 (MQ=255)           aaataatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaatt                                                             <  2:132429/75‑1 (MQ=255)            aataatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaatta                                                            <  2:3282177/76‑1 (MQ=255)             ataatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaattaa                                                           >  2:1375212/1‑76 (MQ=255)             ataatTCACGCGGTAAGGCGAGCGGAATTGATTATCCGTTCGTCTACCGATAAAAAAGAAGCGGTGTTgtaattaa                                                           >  2:1441023/1‑76 (MQ=255)              taatTCACGCGTTAAGGCTAGCGGAATTGATTATATTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAg                                                          >  2:1257418/1‑76 (MQ=255)               aatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAgg                                                         >  2:4440497/1‑76 (MQ=255)               aatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAgg                                                         <  2:224212/76‑1 (MQ=255)               aatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAgg                                                         >  2:269880/1‑76 (MQ=255)               aatTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAgg                                                         <  2:2114541/76‑1 (MQ=255)                atTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGa                                                        >  1:3115220/1‑76 (MQ=255)                 ttCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAg                                                       >  1:3721342/1‑76 (MQ=255)                   cACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTg                                                     <  2:1554306/76‑1 (MQ=255)                   cACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTg                                                     >  2:473107/1‑76 (MQ=255)                     cgcgTTAAGGCTAGCGGCATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGa                                                   <  2:3526914/76‑1 (MQ=255)                     cgcgTTAAGGCTAGCGGAATTGATTAGCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGa                                                   >  2:1081180/1‑76 (MQ=255)                      gcgTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaa                                                  >  1:497372/1‑76 (MQ=255)                      gcgTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaa                                                  <  2:3823548/76‑1 (MQ=255)                        gTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaaaa                                                <  1:2069819/76‑1 (MQ=255)                        gTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaaaa                                                >  1:1075182/1‑76 (MQ=255)                         ttAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAg                                               >  2:3742807/1‑76 (MQ=255)                         ttAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAg                                               >  1:122737/1‑76 (MQ=255)                          tAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAga                                              <  1:4511979/76‑1 (MQ=255)                          tAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAga                                              <  2:4484277/76‑1 (MQ=255)                          tAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAga                                              >  2:107635/1‑76 (MQ=255)                          tAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAga                                              <  1:3037268/76‑1 (MQ=255)                           aaGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgat                                             >  1:4347627/1‑76 (MQ=255)                           aaGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgat                                             >  1:3997263/1‑76 (MQ=255)                             ggCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgatga                                           <  2:2438288/76‑1 (MQ=255)                             ggCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgatga                                           <  2:2869166/76‑1 (MQ=255)                               cTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatc                                         <  1:4776607/76‑1 (MQ=255)                                tAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatca                                        <  2:2367974/76‑1 (MQ=255)                                tAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatca                                        <  1:3615504/76‑1 (MQ=255)                                tAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatca                                        >  2:1589060/1‑76 (MQ=255)                                  gCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatcatc                                      <  2:89411/76‑1 (MQ=255)                                  gCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatcatc                                      >  2:2901129/1‑76 (MQ=255)                                  gCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGatcatc                                      <  1:2371426/76‑1 (MQ=255)                                  gCGGAAGTGATGTTCTTTTCGGAGAAAGAGAGAACTGAAGCGGTGTTTTAAGTAAGGAGGGGAAAAGATGatcatc                                      <  2:94340/76‑1 (MQ=255)                                   cGGAATTGATTATCTTTTCGTATAACAATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCg                                     >  2:3669315/1‑76 (MQ=255)                                    ggAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCgg                                    >  1:2566685/1‑76 (MQ=255)                                    ggAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCgg                                    >  2:2108229/1‑76 (MQ=255)                                        ttGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaa                                                  >  1:689553/1‑58 (MQ=255)                                        ttGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaa                                                  <  2:689553/58‑1 (MQ=255)                                        ttGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAat                                <  2:4660711/76‑1 (MQ=255)                                        ttGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAat                                <  1:4045862/76‑1 (MQ=255)                                         tGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAata                               >  1:469095/1‑76 (MQ=255)                                         tGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAata                               >  1:2932341/1‑76 (MQ=255)                                         tGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAata                               <  2:3617901/76‑1 (MQ=255)                                         tGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAata                               <  2:2406748/76‑1 (MQ=255)                                          gATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAatat                              <  2:1577400/76‑1 (MQ=255)                                           aTTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATAtt                             >  2:2548202/1‑76 (MQ=255)                                           aTTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATAtt                             >  2:4738979/1‑76 (MQ=255)                                            ttATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTc                            <  2:2908408/76‑1 (MQ=255)                                            ttATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTc                            >  1:455553/1‑76 (MQ=255)                                               tCTTTTCGTATAACTATAGAAATGAAGCTTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATa                         <  2:2239337/76‑1 (MQ=255)                                                cTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATaa                        <  2:4347627/76‑1 (MQ=255)                                                 ttttCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAAt                       <  1:4346102/76‑1 (MQ=255)                                                  tttCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATc                      >  1:3572575/1‑76 (MQ=255)                                                   ttCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCt                     <  1:4106069/76‑1 (MQ=255)                                                    tCGTATAACGATAGAGATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATGCTCGGAAATATTCATAATCtt                    <  2:667112/76‑1 (MQ=255)                                                     cGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTc                   <  2:97949/76‑1 (MQ=255)                                                      gTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCa                  >  1:1171994/1‑76 (MQ=255)                                                      gTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCa                  >  2:1044409/1‑76 (MQ=255)                                                       tataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAg                 <  2:2285632/76‑1 (MQ=255)                                                        ataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGc                >  2:915841/1‑76 (MQ=255)                                                        ataACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGc                <  1:2424800/76‑1 (MQ=255)                                                         taACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGcc               >  1:1576649/1‑76 (MQ=255)                                                          aaCGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCg              >  2:4095553/1‑76 (MQ=255)                                                          aaCGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCg              <  2:60768/76‑1 (MQ=255)                                                             gATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTgg           <  2:713965/76‑1 (MQ=255)                                                              aTAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGc          >  1:2674009/1‑76 (MQ=255)                                                              aTAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGc          <  1:1586284/76‑1 (MQ=255)                                                               tAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCt         >  1:4466311/1‑76 (MQ=255)                                                               tAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCt         <  2:3576289/76‑1 (MQ=255)                                                                aGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTa        >  2:1570919/1‑76 (MQ=255)                                                                aGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTa        >  2:2976440/1‑76 (MQ=255)                                                                  aaaTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcc      <  2:4113362/76‑1 (MQ=255)                                                                   aaTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAccc     >  1:3660054/1‑76 (MQ=255)                                                                   aaTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAccc     >  2:578283/1‑76 (MQ=255)                                                                    aTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcccc    <  1:4343134/76‑1 (MQ=255)                                                                    aTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGAGGATCTTCGGAAATATTCATAATCTTCAGCCGTGGCTAcccc    <  1:1441024/76‑1 (MQ=255)                                                                     tGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCa   <  2:1529412/76‑1 (MQ=255)                                                                     tGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCa   <  1:3133583/76‑1 (MQ=255)                                                                     tGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGCTGCTCCCCCa   >  2:1641432/1‑76 (MQ=255)                                                                      gAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAg  <  2:4639166/76‑1 (MQ=255)                                                                         |                                                                         TGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAG  >  NC_000913/4472336‑4472480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑