Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,370	T→C	E28E (GAA→GAG)	yeaA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,370	0	T	C	100.0%	166.0 / NA	63	E28E (GAA→GAG)	yeaA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/38);  total (25/38)

ATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAG  >  NC_000913/1860322‑1860440                                                 |                                                                       cagtaCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaa                                             <  2:2334189/73‑1 (MQ=255) cagtaCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaa                                             <  1:4656919/73‑1 (MQ=255)  agtaCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaaa                                            >  2:931482/3‑76 (MQ=255)   gtaCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGCCTCGGTCCCATGAGTCTGCGTCACGTaaaa                                           >  2:4538582/2‑76 (MQ=255)     aCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACt                                         <  1:1104533/76‑1 (MQ=255)     aCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACt                                         <  1:2195760/76‑1 (MQ=255)      cGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTg                                        <  2:468947/76‑1 (MQ=255)       gCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                       >  2:2404462/1‑76 (MQ=255)       gCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                       <  1:2660393/76‑1 (MQ=255)         cGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAt                                     >  2:394531/1‑76 (MQ=255)          gTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtc                                    <  2:1019267/76‑1 (MQ=255)            cACTCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtctc                                  <  1:634617/76‑1 (MQ=255)            cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtctc                                  <  1:2372430/76‑1 (MQ=255)            cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtctc                                  <  2:541991/76‑1 (MQ=255)            cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtctc                                  <  1:2812273/76‑1 (MQ=255)            cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtctc                                  <  2:1046976/76‑1 (MQ=255)             aCGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                 <  2:2111547/76‑1 (MQ=255)             aCGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                 >  1:4734130/1‑76 (MQ=255)             aCGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                 <  2:433361/76‑1 (MQ=255)             aCGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                 <  2:2619356/76‑1 (MQ=255)              cGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCgg                                <  2:2604888/76‑1 (MQ=255)               gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTACAACTGCATCTCggc                               >  2:2223447/1‑75 (MQ=255)               gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                               >  2:2215273/1‑76 (MQ=255)               gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                               >  1:1793529/1‑76 (MQ=255)               gCTTGTTAGGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                               <  2:146610/76‑1 (MQ=255)                cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCggcc                              >  2:4606598/1‑74 (MQ=255)                cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGAc                              >  2:4544902/1‑76 (MQ=255)                cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGAc                              >  1:133864/1‑76 (MQ=255)                 ttGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACa                             <  1:765392/76‑1 (MQ=255)                 ttGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACa                             >  1:2826965/1‑76 (MQ=255)                 ttGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACa                             >  2:787423/1‑76 (MQ=255)                  tGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACaa                            <  1:898018/76‑1 (MQ=255)                  tGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACaa                            >  2:4761912/1‑76 (MQ=255)                     tATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtt                         >  1:3227864/1‑76 (MQ=255)                      aTGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttt                        <  2:2582429/76‑1 (MQ=255)                      aTGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCGCGGACAAAttt                        <  2:2608900/76‑1 (MQ=255)                       tGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttt                       <  2:3308638/76‑1 (MQ=255)                         cAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                     <  1:3862545/76‑1 (MQ=255)                          aGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttttttt                    <  1:3408015/76‑1 (MQ=255)                            tAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTc                  >  1:3656575/1‑76 (MQ=255)                            tAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTc                  <  1:2419779/76‑1 (MQ=255)                              aaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAg                <  1:1544617/76‑1 (MQ=255)                                cGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGtt              <  2:4787854/76‑1 (MQ=255)                                 gACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttt             >  1:103528/1‑75 (MQ=255)                                 gACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttc             >  1:1241554/1‑76 (MQ=255)                                  gcccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAACTTTTTTTTCAGttct            <  1:3272667/75‑1 (MQ=255)                                   cccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttctt           <  1:3064767/76‑1 (MQ=255)                                    ccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc          <  2:3043214/76‑1 (MQ=255)                                     cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct         <  1:2577422/76‑1 (MQ=255)                                     cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct         >  2:392639/1‑76 (MQ=255)                                     cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct         <  2:1735202/76‑1 (MQ=255)                                     cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct         <  1:4846968/76‑1 (MQ=255)                                     cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct         <  2:4595908/76‑1 (MQ=255)                                      ggaaaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTg        <  2:240496/74‑1 (MQ=255)                                      gTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTCCAACTGCATCTCGGACAAATTTTTTTTCAGTTTTTCTg        >  2:4328722/1‑76 (MQ=255)                                      gTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTg        >  2:4282109/1‑76 (MQ=255)                                      gTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTg        >  1:2370583/1‑76 (MQ=255)                                       tAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGc       <  1:55868/76‑1 (MQ=255)                                        aaaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc      >  2:2756910/1‑76 (MQ=255)                                        aaaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGcc      >  2:2728582/1‑76 (MQ=255)                                          aTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGa    >  2:1403881/1‑76 (MQ=255)                                          aTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGa    <  2:4068875/76‑1 (MQ=255)                                          aTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGa    <  1:1245743/76‑1 (MQ=255)                                           gggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCGCGGACAAATTTTTTTTCAGTTCTTCTGCCGaa   <  2:195356/75‑1 (MQ=255)                                            ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGaac  >  2:199771/1‑75 (MQ=255)                                                 |                                                                       ATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAG  >  NC_000913/1860322‑1860440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑