Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,043,272 A→G S130G (AGT→GGT)  b1978 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,043,2720AG100.0% 336.6 / NA 125S130G (AGT→GGT) b1978hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/75);  total (50/75)

Only 100 of 125 total aligned reads displayed.

AAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG  >  NC_000913/2043199‑2043343
                                                                         |                                                                       
 aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtt                                                                      <  1:3952897/76‑1 (MQ=255)
 aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtt                                                                      <  2:4169582/76‑1 (MQ=255)
  gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                     >  2:4148287/1‑76 (MQ=255)
   cGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtttt                                                                    <  1:2769039/76‑1 (MQ=255)
    gTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTg                                                                   >  1:4393845/1‑76 (MQ=255)
     ttGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGa                                                                  <  1:1290063/76‑1 (MQ=255)
      tGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTTAt                                                                 >  2:402760/1‑76 (MQ=255)
       gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCTTACGTTTGCTCGAGGTTTTGATa                                                                <  1:1771560/76‑1 (MQ=255)
       gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                >  1:3792101/1‑76 (MQ=255)
       gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                >  2:4558970/1‑76 (MQ=255)
       gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                <  2:3089615/76‑1 (MQ=255)
        ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTATGCTCGAGGTTTTGATaa                                                               >  2:1896792/1‑76 (MQ=255)
         cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                              >  2:528506/1‑76 (MQ=255)
           aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGt                                                            >  1:260329/1‑76 (MQ=255)
            aCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGATTCGTACGTTTGGTCGTGGTTTTGATAAttac                                                           >  1:1293937/1‑73 (MQ=255)
              gtgtCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                         <  2:2882720/73‑1 (MQ=255)
              gTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                         >  1:4827047/1‑76 (MQ=255)
              gTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                         <  1:3434369/76‑1 (MQ=255)
               tttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                        <  1:2144857/76‑1 (MQ=255)
               tttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                        <  2:1889886/76‑1 (MQ=255)
                ttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                       >  1:2391182/1‑76 (MQ=255)
                   ggTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                    >  2:4200415/1‑76 (MQ=255)
                   ggTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                    <  2:2628260/76‑1 (MQ=255)
                     tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgggg                                                  >  1:2880448/1‑75 (MQ=255)
                      aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                 <  1:2270487/76‑1 (MQ=255)
                      aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                 <  1:2262368/76‑1 (MQ=255)
                         tCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                              <  1:3482968/76‑1 (MQ=255)
                           ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGaa                                            >  1:4483206/1‑76 (MQ=255)
                           ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGaa                                            <  1:1969663/76‑1 (MQ=255)
                             tGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                          >  2:1063043/1‑76 (MQ=255)
                             tGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                          >  2:3836431/1‑76 (MQ=255)
                             tGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                          <  1:3160341/76‑1 (MQ=255)
                              ggCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                         >  2:3827999/1‑76 (MQ=255)
                              ggCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                         >  1:2620831/1‑76 (MQ=255)
                                gtGAGGTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGa                                       <  2:360556/75‑1 (MQ=255)
                                cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGa                                       >  1:532662/1‑76 (MQ=255)
                                cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGa                                       <  2:1783856/76‑1 (MQ=255)
                                atGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGa                                       >  1:1883316/2‑76 (MQ=255)
                                 tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAt                                      <  2:3879892/76‑1 (MQ=255)
                                  gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGCACTGGATg                                     >  2:4232544/1‑76 (MQ=255)
                                   aGTTACGCAAACTCAACCAGTTTCGTACGTCTGCTCGAGGTTTTGATAATCTCCGACGGGGTGATGAACTGGCTGt                                    >  2:3479405/1‑76 (MQ=255)
                                    gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGCAAATGTCCGCCAGGGTTATGAACTGGATGTc                                   <  1:2863828/76‑1 (MQ=255)
                                     ttACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTca                                  <  2:1224687/76‑2 (MQ=255)
                                     ttACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGGGATGAACTGGATGtgc                                  >  2:126797/1‑74 (MQ=255)
                                       aCGCAAACTCAACCAGTTTCGTCCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCg                                <  2:1586509/76‑1 (MQ=255)
                                       aCGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCg                                <  2:1507985/76‑1 (MQ=255)
                                       aCGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCg                                <  2:807348/76‑1 (MQ=255)
                                       aCGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCg                                <  1:766720/76‑1 (MQ=255)
                                         gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                              <  2:483385/76‑1 (MQ=255)
                                         gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                              >  1:151099/1‑76 (MQ=255)
                                          cAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGca                             <  2:3252276/76‑1 (MQ=255)
                                          cAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGca                             <  2:3175197/76‑1 (MQ=255)
                                           aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcac                            >  2:3577695/1‑76 (MQ=255)
                                            aaCTCACCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGccca                           <  2:4405387/76‑4 (MQ=255)
                                            aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcaca                           <  2:4428189/76‑1 (MQ=255)
                                            aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcaca                           <  1:3818490/76‑1 (MQ=255)
                                             aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                          <  2:3311288/76‑1 (MQ=255)
                                             aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                          <  1:4148287/76‑1 (MQ=255)
                                              cTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAg                         <  1:1786022/76‑1 (MQ=255)
                                               tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                        <  1:3170242/76‑1 (MQ=255)
                                               tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                        >  1:322197/1‑76 (MQ=255)
                                               tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                        <  1:552779/76‑1 (MQ=255)
                                                cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGtt                       <  2:1020179/76‑1 (MQ=255)
                                                cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGtt                       <  1:4594446/76‑1 (MQ=255)
                                                 aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                      >  2:3912190/1‑76 (MQ=255)
                                                 aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                      <  2:1908326/76‑1 (MQ=255)
                                                  aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGttcg                     >  2:3718406/1‑74 (MQ=255)
                                                   ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                    <  2:2448525/76‑1 (MQ=255)
                                                   ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                    <  1:1523669/76‑1 (MQ=255)
                                                   ccAGTTTCGTACGTTTGCGCGAGGTTTGGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                    <  2:1385089/76‑1 (MQ=255)
                                                    cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTg                   <  1:4328603/76‑1 (MQ=255)
                                                      gTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaa                 <  1:649554/76‑1 (MQ=255)
                                                      gTTTCGTACATTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaa                 <  1:1296752/76‑1 (MQ=255)
                                                       tttCGTCCGTGTGCTCGAGGATTTGCTAATGTCCGCCAGGGTGAGGAACTGGGTGTCGCGGCGCAAGTTAGTGaaa                <  2:4123106/76‑1 (MQ=255)
                                                        ttCGTACGTTTGCTCGCGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGCCCCGTCACAAGTTAGTGaaaa               <  2:3871662/76‑1 (MQ=255)
                                                        ttCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaa               <  1:415272/76‑1 (MQ=255)
                                                         tCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa              <  1:26879/76‑1 (MQ=255)
                                                           gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaa            >  1:1027696/1‑76 (MQ=255)
                                                           gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaa            >  1:1769371/1‑76 (MQ=255)
                                                           gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaa            >  2:1225704/1‑76 (MQ=255)
                                                            tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaa           <  1:4463913/76‑1 (MQ=255)
                                                             aCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaaa          <  2:3642406/76‑2 (MQ=255)
                                                              cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAtt         >  2:4217212/1‑76 (MQ=255)
                                                              cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAtt         >  1:913394/1‑76 (MQ=255)
                                                               gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTa        <  2:3361479/76‑1 (MQ=255)
                                                               gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTa        <  2:2470944/76‑1 (MQ=255)
                                                               gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAATa        >  2:3164950/1‑76 (MQ=255)
                                                                tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa       >  1:4260953/1‑76 (MQ=255)
                                                                 ttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAc      <  1:3446615/76‑1 (MQ=255)
                                                                  tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACCAGTTAGGGAAAAAAAATAAAcc     >  2:151621/1‑76 (MQ=255)
                                                                  tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc     <  2:2550247/76‑1 (MQ=255)
                                                                  tGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc     <  2:4221483/76‑1 (MQ=255)
                                                                   gCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc    >  1:569984/1‑76 (MQ=255)
                                                                   gCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc    <  1:3836431/76‑1 (MQ=255)
                                                                   gCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc    >  2:1175779/1‑76 (MQ=255)
                                                                   gCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc    <  1:788856/76‑1 (MQ=255)
                                                                   gCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc    <  2:727832/76‑1 (MQ=255)
                                                                    cTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc   >  1:2067420/1‑76 (MQ=255)
                                                                     tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccg  <  2:1120660/76‑1 (MQ=255)
                                                                     tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccg  <  2:1080478/76‑1 (MQ=255)
                                                                         |                                                                       
AAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG  >  NC_000913/2043199‑2043343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑