BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,216,746	G→C	S252S (TCC→TCG)	yehZ ←	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,216,746	0	G	C	100.0%	315.0 / NA	120	S252S (TCC→TCG)	yehZ	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (53/67); total (53/67)

Only 100 of 120 total aligned reads displayed.

ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCC  >  NC_000913/2216676‑2216817

aTGTTTTTGCGTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc                                                                    <  2:2862081/76‑1 (MQ=255)

aTGTTTTTGCATCGAGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTc                                                                    <  1:3639641/76‑1 (MQ=255)

   tttttGCATCGAGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACg                                                                 <  2:4698189/76‑1 (MQ=255)

      ttGCATCGAGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcac                                                              <  1:249499/76‑1 (MQ=255)

       tGCATCGGGGAGGGTGAAGACTGGCTTTGTCCACTGTGCCATTTTCGGCTGCTCCCTCAACACCGACTCACGcacc                                                             <  2:4711300/76‑1 (MQ=255)

       tGCATCGAGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcacc                                                             <  2:2531246/76‑1 (MQ=255)

        gCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcacca                                                            <  1:1820244/76‑1 (MQ=255)

         cATCGAGGCTGGCGAAGACTGGCTGGAGCCACTGTGCCCTTTTCGGGTACTCCCCCCACAACCACTCACCcaccac                                                           >  1:3386290/1‑76 (MQ=255)

          aTCGAGGCGGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACt                                                          <  1:2541567/76‑1 (MQ=255)

           tCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTg                                                         <  1:2264016/76‑1 (MQ=255)

            cGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTgg                                                        <  1:1160084/76‑1 (MQ=255)

             ggggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGt                                                       <  2:4289773/74‑1 (MQ=255)

              gggcgggcgAAGACGGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTg                                                      <  1:573991/75‑1 (MQ=255)

               ggCTGGCGAAGACTGGCTGGAGCCACTGTGGCCTTTTCGGGTACTCCCCCCACACCCACCCACGCCCCACCTGTGc                                                     >  1:3006035/1‑76 (MQ=255)

                cttGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCa                                                    <  1:2160269/74‑1 (MQ=255)

                 cTGGCGAAGACTGGCTGTAGCCACTGTGCCCTTTTCGGATACTCCCCCAACACCCACTCACCCCCCCCCGGTGccg                                                   >  2:1721840/1‑74 (MQ=255)

                  gggCGAGGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAgg                                                  <  2:1284206/75‑1 (MQ=255)

                    gCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGgcg                                                <  1:872335/76‑1 (MQ=255)

                    gCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCCACTCACGCACCACCGGTGCAGgcg                                                >  2:3579923/1‑76 (MQ=255)

                     cGAAGACTGGCTGGAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCCACTCACGCACCACCGGTGCAGgcgc                                               >  1:3089457/1‑76 (MQ=255)

                      gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCCACACCGACTCACCCCCCCCCGGTGCAcgcgcg                                              >  2:2631497/1‑76 (MQ=255)

                      gAAGACTGGCTGGAGCCACTGTGCCATTTGCGGGTACTTCCTCAACACCCACTCACGCACCACTGGTGCAGGcgcg                                              >  1:3444975/1‑76 (MQ=255)

                      gAAGACTGCCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGcgcg                                              <  1:4274097/76‑1 (MQ=255)

                       aaGACTGGCTGTAGCCCCTGTGCCATTTTCGGGTACTCCCTCAACCCCCACTCCCCCACCACCGGGGCCGGcgcgg                                             >  2:695391/1‑75 (MQ=255)

                       aaGACTAGCTGCAGCCACTGTGCCATTTTCGGATACTCCCCCACCACCCCCTCACGCACCCCTGGTGCCGtcgccc                                             >  2:1647516/1‑74 (MQ=255)

                        aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTa                                            <  1:132628/76‑1 (MQ=255)

                        aGACTGGCTGGAGCCACTGTGCCATTTGCGGGTACTCCCTCAACACCCACTCACCCCCCCCCGGGGCAGGcgcggg                                            >  1:1658177/1‑74 (MQ=255)

                         gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACCGGTGCAGGCGCGTAg                                           >  2:3246710/1‑76 (MQ=255)

                          gcTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGa                                          <  2:4579638/75‑1 (MQ=255)

                          aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGa                                          <  1:4560409/76‑1 (MQ=255)

                          aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCCCCAAAACCACTCACGCACCACCGGGGCCAGCGCGgaga                                          >  1:1095246/1‑76 (MQ=255)

                            tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                        <  1:3180846/76‑1 (MQ=255)

                            tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACAACGACTCACGCACCACTGGCGCAGGCGCGGAGATa                                        >  1:2163157/1‑76 (MQ=255)

                             ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAg                                       >  2:3332544/1‑76 (MQ=255)

                                tGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                    >  2:2216694/1‑76 (MQ=255)

                                  tAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGc                                  <  2:3771786/76‑1 (MQ=255)

                                   aGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                 <  1:705003/76‑1 (MQ=255)

                                   aGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGCTGca                                 >  1:3179945/1‑76 (MQ=255)

                                   aGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGCGCAGCCGCGCAGATAGCGTGca                                 >  2:626165/1‑76 (MQ=255)

                                     ccACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGGAGATAGGTTGcaca                               >  2:1311636/1‑76 (MQ=255)

                                      cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac                              >  2:2917205/1‑76 (MQ=255)

                                      cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac                              >  2:788190/1‑76 (MQ=255)

                                      cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGCTGCacac                              >  1:822775/1‑76 (MQ=255)

                                        cTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCt                            >  1:1446553/1‑76 (MQ=255)

                                        cTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCt                            <  1:799423/76‑1 (MQ=255)

                                         tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                           <  1:4133302/76‑1 (MQ=255)

                                         tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                           <  1:4103271/76‑1 (MQ=255)

                                         cgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                           <  1:303278/75‑1 (MQ=255)

                                          gtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                          <  1:1694485/76‑1 (MQ=255)

                                          gtgCCATTTGCGGATACTCCCCCAACACCGACTCACACACCACCGGCGCACGCGCGCAGATAGGTTGCACACCTTg                          >  1:4193129/1‑76 (MQ=255)

                                           tgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGCGCAGGCGCGTAGATAGGTTGCACACCtttc                         >  2:4722861/1‑74 (MQ=255)

                                              cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                      <  1:2486846/76‑1 (MQ=255)

                                              cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                      <  1:711265/76‑1 (MQ=255)

                                              cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                      >  1:3881175/1‑76 (MQ=255)

                                              cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                      >  2:2550807/1‑76 (MQ=255)

                                               aTTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAt                     >  2:1997612/1‑76 (MQ=255)

                                               aTTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAt                     <  1:3562146/76‑1 (MQ=255)

                                                tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                    <  2:4321998/76‑1 (MQ=255)

                                                tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                    <  1:3153178/76‑1 (MQ=255)

                                                  tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGGAGATAGGTTGCACACCTTGCGGATCGc                  >  2:2315821/1‑76 (MQ=255)

                                                   gCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCt                 <  2:2211209/76‑1 (MQ=255)

                                                    gggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCtt                <  2:131501/75‑1 (MQ=255)

                                                    cGGATCCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCtt                <  2:1192159/76‑1 (MQ=255)

                                                     ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa               <  1:1869310/76‑1 (MQ=255)

                                                     ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa               <  1:4558893/76‑1 (MQ=255)

                                                     ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa               <  2:3879179/76‑1 (MQ=255)

                                                     ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa               <  1:2790981/76‑1 (MQ=255)

                                                     ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTa               >  1:3309175/1‑76 (MQ=255)

                                                      gATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTaa              <  2:1293743/76‑1 (MQ=255)

                                                       gtgcTCCCTCAACACCGGCTCACGCCCCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg             <  2:104759/73‑1 (MQ=255)

                                                       aTACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg             >  2:3187695/1‑76 (MQ=255)

                                                       aTACTCCCTCAACACCGACTAACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg             <  2:2280202/76‑1 (MQ=255)

                                                         aCTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGt           >  2:2701687/1‑76 (MQ=255)

                                                          cTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt          <  2:2521676/76‑1 (MQ=255)

                                                          cTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt          <  1:1428892/76‑1 (MQ=255)

                                                          cTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCCTGCGGCTCGCCTAAGGtt          >  2:258454/1‑76 (MQ=255)

                                                           tCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt         >  2:2301747/1‑76 (MQ=255)

                                                           tCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt         <  2:2092467/76‑1 (MQ=255)

                                                           tCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGttt         >  1:412927/1‑76 (MQ=255)

                                                             ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGc       >  2:2817017/1‑76 (MQ=255)

                                                              cgcAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa      <  1:1005750/74‑1 (MQ=255)

                                                              cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGcc      >  2:696970/1‑75 (MQ=255)

                                                              cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa      <  1:4311423/76‑1 (MQ=255)

                                                              cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa      <  1:3035648/76‑1 (MQ=255)

                                                              cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa      <  1:1575245/76‑1 (MQ=255)

                                                              cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTAGCACACCTTGCGGATCGCTTAAGGTTTGCa      >  1:2310034/1‑76 (MQ=255)

                                                               tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAg     >  1:4756360/1‑76 (MQ=255)

                                                               ccAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAg     <  2:566447/75‑1 (MQ=255)

                                                                cAACACCGACTCACGCACCACTGGTGCAGGCGCGTCGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc    >  2:1323267/1‑76 (MQ=255)

                                                                cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc    <  2:3222468/76‑1 (MQ=255)

                                                                cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc    <  2:3370338/76‑1 (MQ=255)

                                                                cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc    <  1:2859652/76‑1 (MQ=255)

                                                                 aaCACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc   >  2:2108937/1‑76 (MQ=255)

                                                                 aaCACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc   <  2:4749185/76‑1 (MQ=255)

                                                                  acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc  >  1:3062475/1‑76 (MQ=255)

                                                                  acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc  >  1:4148401/1‑76 (MQ=255)

                                                                  acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc  <  1:4781954/76‑1 (MQ=255)

                                                                  acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc  <  1:1963798/76‑1 (MQ=255)

                                                                  acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc  <  1:1185193/76‑1 (MQ=255)

                                                                  acacCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGccc  >  2:3087967/1‑76 (MQ=255)

ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCC  >  NC_000913/2216676‑2216817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`