BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,691,024	A→T	F847Y (TTT→TAT)	purL ←	phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,691,024	0	A	T	100.0%	545.6 / NA	178	F847Y (TTT→TAT)	purL	phosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (86/92); total (86/92)

Only 100 of 184 total aligned reads displayed.

CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTA  >  NC_000913/2690951‑2691095

cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCACGCTGCCGataa                                                                       >  2:188771/1‑76 (MQ=255)

 tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataaa                                                                      <  2:2259227/76‑1 (MQ=255)

 tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataaa                                                                      >  1:3183062/1‑76 (MQ=255)

  gAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAc                                                                     >  2:3524475/1‑76 (MQ=255)

  gAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAc                                                                     >  2:4325418/1‑76 (MQ=255)

  gAAGCGGGTCATGGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAc                                                                     <  2:297839/76‑1 (MQ=255)

   aaTCGCGTCATAGAAGCCTTTCATTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAcc                                                                    <  2:1325521/76‑1 (MQ=255)

   aaTCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAcc                                                                    >  2:2910201/1‑76 (MQ=255)

    aTCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCt                                                                   <  1:1716190/76‑1 (MQ=255)

     tCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTAGAGCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTg                                                                  <  2:3108604/76‑1 (MQ=255)

      ggcgTCATAGAAGCGTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgc                                                                 <  2:3550848/75‑1 (MQ=255)

      cgcgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTCGTCGCCAAGCTGAAGATAATCCTgc                                                                 >  2:2050355/1‑76 (MQ=255)

       gcgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgcg                                                                >  1:3501136/1‑76 (MQ=255)

       gcgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgcg                                                                <  2:2750404/76‑1 (MQ=255)

        cgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgcgc                                                               <  2:2389139/76‑1 (MQ=255)

        cgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgcgc                                                               <  1:4184071/76‑1 (MQ=255)

            aTAGAGGCCTTTCGGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                           <  1:639424/76‑1 (MQ=255)

            aTAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCCgc                                                           >  1:702527/1‑76 (MQ=255)

            aTAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                           <  1:2109556/76‑1 (MQ=255)

            aTAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                           <  1:54005/76‑1 (MQ=255)

             tAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcg                                                          >  1:3137499/1‑76 (MQ=255)

              aGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcgc                                                         <  2:4155305/76‑1 (MQ=255)

              aGAAGCCTTTCAGTTGCGCGACATCGAGTACATCTGCCGGTTTGTCGCCCAGCTGACGATAAACCTGCGCCAgcgc                                                         >  2:4193205/1‑76 (MQ=255)

                aaGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCg                                                       <  1:4327947/76‑1 (MQ=255)

                   ccTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTg                                                    <  2:2019009/76‑1 (MQ=255)

                   ccTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGATg                                                    >  1:1159958/1‑76 (MQ=255)

                    cTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGtttc                                                   >  1:95008/1‑74 (MQ=255)

                    cTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                                   >  1:2165070/1‑76 (MQ=255)

                    cTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                                   <  1:888858/76‑1 (MQ=255)

                    cTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                                   >  1:3627543/1‑76 (MQ=255)

                    cTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                                   >  1:4622079/1‑76 (MQ=255)

                    cTTGCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                                   <  2:3924399/76‑1 (MQ=255)

                      ttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcgc                                                 <  1:2278231/76‑1 (MQ=255)

                         aGTTGCGCGACATCGCGTACATCTGCCTGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCa                                              <  2:3124934/76‑1 (MQ=255)

                         aGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCa                                              >  1:361873/1‑76 (MQ=255)

                         aGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCa                                              <  1:3765117/76‑1 (MQ=255)

                          gTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAg                                             >  1:4400530/1‑76 (MQ=255)

                          gTTGCGCGACAGCGCGTACATCTGCGGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAg                                             <  2:3772496/76‑1 (MQ=255)

                           ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgc                                            <  1:1322574/76‑1 (MQ=255)

                           ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgc                                            <  1:2206919/76‑1 (MQ=255)

                            tGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcg                                           <  1:1015325/76‑1 (MQ=255)

                            tGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcg                                           <  1:3137072/76‑1 (MQ=255)

                            tGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcg                                           <  1:2309570/76‑1 (MQ=255)

                             gcgcGACATCGCGTCCATCTGCCGGTTGGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCACCCAgcgc                                          <  2:1406853/76‑1 (MQ=255)

                             gcgcGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                          <  2:2298504/76‑1 (MQ=255)

                             gcgcGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                          <  1:1456668/76‑1 (MQ=255)

                             gcgcGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                          >  1:155883/1‑76 (MQ=255)

                             gcgcGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                          >  2:1172710/1‑76 (MQ=255)

                              cgcgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCcgcgcg                                         >  2:932993/1‑76 (MQ=255)

                               gcgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCCGCGCGt                                        >  1:199208/1‑76 (MQ=255)

                                cgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGCTGCGCCCAGCGCGtt                                       >  1:719861/1‑76 (MQ=255)

                                 gACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTa                                      >  1:264808/1‑76 (MQ=255)

                                 gACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGCTGCGCCCAGCGCGTTa                                      >  2:4717352/1‑76 (MQ=255)

                                 gACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGCTGCGCCCAGCGCGCTa                                      >  2:162621/1‑76 (MQ=255)

                                  aCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGCCGCTACACCTGCGCCCGCGCCGTTGCGCCCCCCCCGTTAt                                     >  2:535508/1‑76 (MQ=255)

                                  aCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                     <  1:3705146/76‑1 (MQ=255)

                                  aCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                     >  2:488852/1‑76 (MQ=255)

                                      cgcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                 <  1:3313552/76‑1 (MQ=255)

                                      cgcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                 <  2:848153/76‑1 (MQ=255)

                                      cgcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                 >  1:4275092/1‑76 (MQ=255)

                                       gcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCt                                <  2:1656328/76‑1 (MQ=255)

                                       gcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCt                                >  1:3401394/1‑76 (MQ=255)

                                        cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCtt                               <  1:1087247/76‑1 (MQ=255)

                                        cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATCGCCtt                               >  2:3319944/1‑76 (MQ=255)

                                        cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGGTATTTCCtt                               >  1:1835617/1‑76 (MQ=255)

                                         gTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCttt                              >  1:4825597/1‑76 (MQ=255)

                                         gTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCttt                              <  1:1617980/76‑1 (MQ=255)

                                          tACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTg                             >  2:3518330/1‑76 (MQ=255)

                                          gaCATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTg                             <  2:2481495/75‑1 (MQ=255)

                                           aCATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTACTGCCTTTGc                            >  1:3067163/1‑76 (MQ=255)

                                            cATCTGCCGGGTTGTCGACAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc                           >  1:4769854/1‑76 (MQ=255)

                                               cTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCaa                        <  1:1047803/76‑1 (MQ=255)

                                                ggCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCaaa                       <  2:698518/75‑1 (MQ=255)

                                                 gCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaat                      >  1:1994171/1‑76 (MQ=255)

                                                  ccGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatc                     <  1:1650225/76‑1 (MQ=255)

                                                  ccGGTTTGTCGCCAAGCTGACGATAAAACTGCGCCAGCGCCGGCACGCCCAGCGCGATATTGCCTTTTCCCaaaac                     >  1:2459878/1‑74 (MQ=255)

                                                  ccGGTTGGGCCCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatc                     <  2:399532/76‑1 (MQ=255)

                                                   cGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatca                    <  2:2126536/76‑1 (MQ=255)

                                                   cGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatca                    <  1:4753914/76‑1 (MQ=255)

                                                   cGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatca                    >  1:38446/1‑76 (MQ=255)

                                                     gTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaat                  <  2:3501136/76‑1 (MQ=255)

                                                      tttGTCGACAAGCTGACGATAAACCTGCGCCAGCGCCGCTCCGCCCAGCGCGTTATTGCCTTTCCCCAaatccatc                 >  2:4573285/1‑76 (MQ=255)

                                                        tGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcag               >  1:1918090/1‑76 (MQ=255)

                                                         gTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc              >  1:926606/1‑76 (MQ=255)

                                                         gTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc              >  2:2492322/1‑76 (MQ=255)

                                                          tCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagca             <  2:1944182/76‑1 (MQ=255)

                                                            gCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagc           >  2:1661783/1‑76 (MQ=255)

                                                             ccAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagca          >  1:3431190/1‑76 (MQ=255)

                                                               aaGCTGACGCTCAACCTGCGCCAGCGCCGTTGCGCCCAGCGCTTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt        <  1:2007892/76‑1 (MQ=255)

                                                                aGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTg       >  2:4528568/1‑76 (MQ=255)

                                                                 gCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGc      <  2:630569/76‑1 (MQ=255)

                                                                 gCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCACCAATCAATCAGGAGTAGTGc      >  1:775185/1‑76 (MQ=255)

                                                                  cTGACGATAACCCGGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCg     <  2:47337/76‑1 (MQ=255)

                                                                  cTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCg     >  1:4172707/1‑76 (MQ=255)

                                                                   tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGt    <  2:183022/76‑1 (MQ=255)

                                                                   tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGt    >  2:1450813/1‑76 (MQ=255)

                                                                   tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGt    <  2:2583714/76‑1 (MQ=255)

                                                                    gACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGtt   >  1:3016861/1‑76 (MQ=255)

                                                                    gACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGtt   <  2:1463546/76‑1 (MQ=255)

                                                                     aCGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa  <  1:2303658/76‑1 (MQ=255)

CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTA  >  NC_000913/2690951‑2691095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`