| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,766,423 | G→A | R231R (CGG→CGA) | b2632 → | putative GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,766,423 | 0 | G | A | 99.1% | 330.6 / NA | 112 | R231R (CGG→CGA) | b2632 | putative GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (54/57); minor base C (1/0); total (55/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
CAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTA > NC_000913/2766350‑2766492 | cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAAc > 2:1439964/1‑76 (MQ=255) agagCGGCTGATTAACTGTCTGCCGCGTGAGGCCCGCAACCCGGAACACGCGCTACTCCAACACCCCTTTCGcaca > 2:3792458/1‑76 (MQ=255) agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACa < 2:2136441/76‑1 (MQ=37) agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACa > 1:3034475/1‑76 (MQ=255) gagGGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaa < 2:1265075/76‑2 (MQ=25) gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaa < 2:1927506/76‑2 (MQ=37) gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaa > 1:3106874/1‑75 (MQ=37) gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaa > 1:697607/1‑75 (MQ=37) gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaa > 1:2721754/1‑75 (MQ=37) gagCGGATGATTAAGGGGCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaa < 2:294162/76‑2 (MQ=25) agCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaac < 1:4096769/76‑3 (MQ=37) gCGGATGATTACGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTCCTGCAACATCCCTTTCGAACaacg < 1:2698936/76‑4 (MQ=255) gCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaacg > 1:931585/1‑73 (MQ=37) atgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTac > 1:29788/1‑75 (MQ=255) tgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGc > 2:2252112/1‑76 (MQ=255) atTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc > 1:4851648/1‑76 (MQ=255) atTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc < 2:2444119/76‑1 (MQ=255) tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGACCAACGGTAGCCCgg > 2:1898399/1‑76 (MQ=255) tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCgg < 1:1835704/76‑1 (MQ=255) aaGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg < 2:4164043/76‑1 (MQ=255) aaGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg > 1:699185/1‑76 (MQ=255) aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGa > 1:2049310/1‑76 (MQ=255) aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGa < 2:3559695/76‑1 (MQ=255) gtgtCTGCCGCGTGAGGCCAGCAGCCAGGTAGTAGCGCGACTGCAACATCCCTCTACAACAACGGTAGCCCGGGAg > 1:2733471/1‑76 (MQ=255) tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaacg > 2:1062811/1‑60 (MQ=255) tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACaacg < 1:1062812/63‑4 (MQ=255) tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGc < 1:1799693/76‑1 (MQ=255) gtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa < 1:3184453/76‑1 (MQ=255) ggcTGGCGGGTGAGGCCAGCAGCCGGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa < 2:3906822/74‑1 (MQ=255) tCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAg > 1:4611817/1‑76 (MQ=255) cTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAgg > 2:642224/1‑76 (MQ=255) cTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAgg < 2:3006228/76‑1 (MQ=255) tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc < 1:3605231/76‑1 (MQ=255) tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc < 1:295051/76‑1 (MQ=255) tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc > 2:3581223/1‑76 (MQ=255) tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCCGGAGCCGGc > 2:815731/1‑76 (MQ=255) gCCGCGTGAGGCCAGCAGCCCGGTAGTCGCGCTCCTGCCACATCCCTTTCGCACAACGGTCGCCCGCGCGCCGGCa > 2:3735971/1‑76 (MQ=255) gCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCCGGCa > 2:3699467/1‑76 (MQ=255) gCCGCGTGAGGCCAGCAGCCCGGTAGCAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCCGGAGCAGGcc > 2:2434427/1‑75 (MQ=255) ccGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc > 2:1667280/1‑76 (MQ=255) cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg > 1:3626745/1‑76 (MQ=255) cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg < 2:1400393/76‑1 (MQ=255) gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCCCgc > 1:472784/1‑75 (MQ=255) gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGt < 2:3266789/76‑1 (MQ=255) gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGt < 1:3609493/76‑1 (MQ=255) ggTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTg < 2:4043374/75‑1 (MQ=255) cgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCCGGAGCAGGCACGTg > 2:3097399/1‑76 (MQ=255) gTGAGGCCAGCAGCCCGGTAGTAGCGCCACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGa > 1:1575152/1‑76 (MQ=255) tGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAc > 1:4706202/1‑76 (MQ=255) gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACg < 2:4247278/76‑1 (MQ=255) aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGCAGCCCCGGAGCAGGTACGTGCCGa > 2:2045602/4‑76 (MQ=255) aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTCCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGa < 1:1255565/73‑1 (MQ=255) ggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCCGGCGCAGGCACCTCACGAt > 2:1135394/3‑76 (MQ=255) ccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGCttt > 2:528178/1‑76 (MQ=255) ccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAttt > 2:882606/1‑76 (MQ=255) ccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAttt < 1:3070247/76‑1 (MQ=255) cagcggCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTc < 2:2206433/76‑1 (MQ=255) cagcagCCCGGTAGTAGCGCTACTGCAAGATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTc < 1:3456179/76‑1 (MQ=255) agcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCg > 2:1716197/1‑76 (MQ=255) agcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCg < 1:2744972/76‑1 (MQ=255) agcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCg > 2:1062879/1‑76 (MQ=255) agcagcCCGGGAGGAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCg < 2:555947/76‑1 (MQ=255) gcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCgg < 1:4519148/76‑1 (MQ=255) agcCCGGTAATAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTg > 2:2441693/1‑76 (MQ=255) cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa < 2:3553424/76‑1 (MQ=255) ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaac > 2:414142/1‑75 (MQ=255) ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa > 1:3291247/1‑76 (MQ=255) cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc > 1:2994936/1‑76 (MQ=255) cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc < 2:3270316/76‑1 (MQ=255) ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc < 1:1701332/76‑1 (MQ=255) ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc > 1:3271395/1‑76 (MQ=255) gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCg > 1:495794/1‑76 (MQ=255) gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCg < 1:3731575/76‑1 (MQ=255) tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCgc > 2:200796/1‑75 (MQ=255) tagtagAGCTAATGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGt > 1:3619064/1‑76 (MQ=255) agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCgg > 2:622132/1‑66 (MQ=255) agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCgg < 1:622132/66‑1 (MQ=255) gtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg < 1:2650043/76‑1 (MQ=255) tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg < 2:3096122/76‑2 (MQ=255) tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg < 1:4222033/76‑2 (MQ=255) gagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg < 2:4569897/75‑2 (MQ=255) agCGCTACCGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggt > 2:2543124/1‑74 (MQ=255) gcgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggtg < 1:2310504/76‑4 (MQ=255) tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt > 2:1268590/1‑76 (MQ=255) tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt > 2:4008873/1‑76 (MQ=255) aCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa < 1:3037021/76‑1 (MQ=255) cTGCAACATCCCTTTCGAGCAGCGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc < 2:709420/76‑1 (MQ=255) cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc < 1:115097/76‑1 (MQ=255) cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc > 2:2472989/1‑76 (MQ=255) tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAACATTTCGGTGAAACCGTAGGTGCCATAcc > 2:2660946/1‑75 (MQ=255) gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc > 2:3612892/1‑76 (MQ=255) cAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg < 1:2361065/76‑1 (MQ=255) caCATCCCTTTCGAACAACTGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa < 2:4027875/75‑1 (MQ=255) aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt < 2:3339371/76‑1 (MQ=255) aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt < 1:3957390/76‑1 (MQ=255) aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt < 2:2527547/76‑1 (MQ=255) cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa < 2:3727075/76‑1 (MQ=255) tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg < 2:1960571/76‑1 (MQ=255) ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgga > 2:1763192/1‑75 (MQ=255) cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa < 1:2493435/76‑1 (MQ=255) | CAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTA > NC_000913/2766350‑2766492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |