| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,766,473 | A→G | D248G (GAT→GGT) | b2632 → | putative GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,766,473 | 0 | A | G | 99.0% | 244.6 / NA | 95 | D248G (GAT→GGT) | b2632 | putative GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (40/54); minor base C (1/0); total (41/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGT > NC_000913/2766404‑2766542 | tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt > 2:4008873/1‑76 (MQ=255)tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt > 2:1268590/1‑76 (MQ=255) aCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa < 1:3037021/76‑1 (MQ=255) cTGCAACATCCCTTTCGAGCAGCGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc < 2:709420/76‑1 (MQ=255) cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc < 1:115097/76‑1 (MQ=255) cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc > 2:2472989/1‑76 (MQ=255) tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAACATTTCGGTGAAACCGTAGGTGCCATAcc > 2:2660946/1‑75 (MQ=255) gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc > 2:3612892/1‑76 (MQ=255) cAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg < 1:2361065/76‑1 (MQ=255) caCATCCCTTTCGAACAACTGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa < 2:4027875/75‑1 (MQ=255) aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa < 2:1085241/76‑1 (MQ=255) aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt < 2:3339371/76‑1 (MQ=255) aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt < 2:2527547/76‑1 (MQ=255) aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt < 1:3957390/76‑1 (MQ=255) cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa < 2:3727075/76‑1 (MQ=255) aTCCCTTTCGACCAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc < 2:2691947/76‑1 (MQ=255) aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc < 1:3202396/76‑1 (MQ=255) tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg < 2:1960571/76‑1 (MQ=255) ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgga > 2:1763192/1‑75 (MQ=255) cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa < 1:2493435/76‑1 (MQ=255) ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc < 1:3846797/76‑1 (MQ=255) ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc < 2:3597408/76‑1 (MQ=255) ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc > 1:2374563/1‑76 (MQ=255) ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc > 2:2229011/1‑76 (MQ=255) ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc > 2:951300/1‑76 (MQ=255) ttAGCCCGGGAGCAGGCACGTGACGATTTCGGTGACACCGTAGGTTCGATACTCGATACTGTAAGCACCTTTcccc > 1:1696229/2‑76 (MQ=255) gTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc > 1:1666668/1‑76 (MQ=255) gTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc < 2:4520238/76‑1 (MQ=255) tAGCCCGGGCGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATGCTCGATCCGGTAAGCACCTTTCCCCt < 2:1236306/76‑1 (MQ=255) tAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTCAGCACCTTTCCCCt > 2:3334695/1‑76 (MQ=255) aGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAGCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt < 1:410439/76‑1 (MQ=255) aGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTATGCACCTTTCCCCtt < 2:1716474/76‑1 (MQ=255) aGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt < 1:3121443/76‑1 (MQ=255) aGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt < 2:160211/76‑1 (MQ=255) aGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACAGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt > 1:3369602/1‑76 (MQ=255) gCCCGGTAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCCCCTTTCCCCTTa < 1:3034632/76‑1 (MQ=255) gCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa > 1:3800015/1‑76 (MQ=255) ccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg > 2:4519304/1‑60 (MQ=255) ccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg < 1:4519304/60‑1 (MQ=255) ccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt > 2:2225406/1‑76 (MQ=255) ccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt < 2:2721754/76‑1 (MQ=255) ccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGCGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtc > 2:3959884/1‑75 (MQ=255) gggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTcc > 1:2733852/1‑76 (MQ=255) gggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTcc > 1:3996105/1‑76 (MQ=255) ggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTccc < 2:1943917/76‑1 (MQ=255) ggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTccc < 1:4039616/76‑1 (MQ=255) aGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGc > 2:3065232/1‑76 (MQ=255) aGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGc > 1:3704531/1‑76 (MQ=255) aGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGc > 1:457018/1‑76 (MQ=255) gCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcc < 1:2781154/76‑1 (MQ=255) gCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcc > 1:3205665/1‑76 (MQ=255) gCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcc < 2:1947266/76‑1 (MQ=255) gCAGGCACGTGACGATTTCGGTGAAACAGCAGCCCCCATACCCGTGACGGCAAGCACCTTGCCCCTCATCcccccc > 2:3390780/1‑76 (MQ=255) cAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGccc > 2:1895930/1‑76 (MQ=255) aGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcccc > 1:418017/1‑76 (MQ=255) aGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcccc < 1:882607/76‑1 (MQ=255) aGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcccc > 2:1898960/1‑76 (MQ=255) aGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcccc < 1:3449198/76‑1 (MQ=255) ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCg < 1:4022283/76‑1 (MQ=255) ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCg < 1:2310609/76‑1 (MQ=255) gCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCgg < 1:528057/76‑1 (MQ=255) ccGTGACGATTCCGGTGCAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTCTTCCCGCCCCGGTg < 1:582230/75‑1 (MQ=255) agttGACGATTTCGGCGCGTCCGTAGGTTCCCTACTCGGTACGGTAACCTCCTTTCCCCTTATTCCCGCCCCCGTg < 1:2409557/73‑1 (MQ=255) cGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGc < 1:4481295/76‑1 (MQ=255) cGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGc < 1:3640951/76‑1 (MQ=39) cGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGc < 1:4335613/76‑1 (MQ=39) cGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGCTGc > 1:723092/1‑76 (MQ=255) cGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGGAAGCACCTTTCCCCTTATTCCCGCCCCGGTGc < 2:714909/76‑1 (MQ=38) gTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCg > 1:4441985/1‑76 (MQ=38) gTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCg > 1:2858359/1‑76 (MQ=38) tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg < 2:3636495/76‑1 (MQ=38) tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg > 1:1151299/1‑76 (MQ=38) ggACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg < 2:2700958/75‑1 (MQ=38) gACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGa > 2:1516985/1‑76 (MQ=38) gACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGa < 1:3771041/76‑1 (MQ=38) gATTTCGGTGAAGTCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGGCCa < 1:3379290/76‑1 (MQ=37) gATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCa < 1:2398316/76‑1 (MQ=38) gATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCa > 1:4481794/1‑76 (MQ=38) aTTTCGGTGAAACCGTAGGTGCGATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCat < 1:259387/76‑1 (MQ=38) tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatt < 1:2330394/76‑1 (MQ=39) tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGAACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatt > 2:4729488/1‑76 (MQ=38) ttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatta > 1:4211106/1‑76 (MQ=39) tCGGTGAAACCTTGGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattat < 2:4644652/76‑1 (MQ=38) tCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattat < 1:944953/76‑1 (MQ=255) tCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattat < 1:973733/76‑1 (MQ=255) tggTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattatt < 2:3369062/75‑1 (MQ=38) cGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattatt > 2:3684880/1‑76 (MQ=39) ggTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTc > 2:525688/1‑76 (MQ=38) tGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAg < 2:3889317/76‑1 (MQ=255) gAAACCGGAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAgg < 2:2134572/76‑1 (MQ=39) aaaCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGc > 2:1429158/1‑76 (MQ=38) aaCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGcc > 2:4002111/1‑76 (MQ=38) aCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCg > 1:2975093/1‑76 (MQ=38) ccGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt > 1:3676304/1‑76 (MQ=38) ccGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt < 2:837168/76‑1 (MQ=255) | TACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGT > NC_000913/2766404‑2766542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |