| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 78,003 | A→C | E128A (GAG→GCG) | yabM → | putative transport protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 78,003 | 0 | A | C | 99.2% | 356.0 / NA | 131 | E128A (GAG→GCG) | yabM | putative transport protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (67/63); minor base G (0/1); total (67/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 134 total aligned reads displayed.
TGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGAGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCT > NC_000913/77931‑78073 | tGACGCTTATCACCTGTGGTGTGCTTCTGGCCTCTCTGGCCACTCCGGCACTGCCACCGATCTTTGCTCCGGcgcg > 1:4871366/1‑76 (MQ=255)tGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGcgcg > 1:750824/1‑76 (MQ=255) aCGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCggg > 1:1184920/1‑76 (MQ=255) cGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGa < 2:3202602/76‑1 (MQ=255) cGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAAGGCCACAGTTATTTGCCCTcgcgcgcca > 2:57035/1‑73 (MQ=255) gCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGaa > 1:1956780/1‑76 (MQ=255) gCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGaa > 1:1607870/1‑76 (MQ=255) cTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAat > 2:4043401/1‑76 (MQ=255) cTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAat < 2:4158359/76‑1 (MQ=255) ttATCACCTGTTGTGTGCTGCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAata < 1:4083863/76‑1 (MQ=255) ttATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGActa > 2:4157689/1‑76 (MQ=255) ttATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAata > 1:4262622/1‑76 (MQ=255) ttATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAata < 1:269672/76‑1 (MQ=255) aTCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATg > 2:3890639/1‑76 (MQ=255) tCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGc < 2:4741228/76‑1 (MQ=255) cACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCg > 2:4467767/1‑76 (MQ=255) cACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCg < 1:918418/76‑1 (MQ=255) aCCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCgg > 2:2742868/1‑76 (MQ=255) aCCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCgg < 1:1011354/76‑1 (MQ=255) ccTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGa > 1:3971713/1‑76 (MQ=255) tgtgGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATa < 2:3438569/76‑1 (MQ=255) gtggtgTGTTGCGGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATaa < 2:9613/76‑1 (MQ=255) gtggtgTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATaa < 1:2544115/76‑1 (MQ=255) gtgtGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTc > 2:1260024/1‑76 (MQ=255) gtgtGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTc < 1:1073583/76‑1 (MQ=255) gtgtGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTc < 1:4040387/76‑1 (MQ=255) gtgtGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTc < 1:3306950/76‑1 (MQ=255) gtgtGCGGCTGGCATCTCTGGCCAAGACGGCAATGCCACAGTTAGTTGCTCTGGCGCGGGAATATGCGGATAAcgc < 1:3343764/76‑3 (MQ=255) tgtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCg > 1:2935474/1‑76 (MQ=255) tgtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCg < 2:2597403/76‑1 (MQ=255) ggggCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCg < 2:283669/73‑1 (MQ=255) gtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGTAATATGCGGATCACTCgg > 2:3198755/1‑76 (MQ=255) gtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCgg > 1:2613822/1‑76 (MQ=255) gtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCgg < 1:3235250/76‑1 (MQ=255) gtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCgg > 2:3195877/1‑76 (MQ=255) tgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAGCTCGgc < 2:111548/76‑1 (MQ=255) tgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgc < 1:3546374/76‑1 (MQ=255) tgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgc < 2:3790164/76‑1 (MQ=255) cTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgcgc > 2:1497178/1‑76 (MQ=255) ttCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGcgcg > 2:965026/1‑76 (MQ=255) ttCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGcgcg > 2:2509453/1‑76 (MQ=255) ttCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGcgcg > 2:3406803/1‑76 (MQ=255) tCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGcgcgg > 1:421535/1‑75 (MQ=255) tCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGcgcgg > 2:2114840/1‑75 (MQ=255) tCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGcgcgg > 2:1779503/1‑75 (MQ=255) tCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCga < 1:2742868/76‑1 (MQ=255) tCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCga < 1:1668641/76‑1 (MQ=255) tCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCga > 2:1425890/1‑76 (MQ=255) cTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCgag > 2:4281117/1‑76 (MQ=255) tGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCgaga > 1:1674531/1‑76 (MQ=255) ggCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGaa < 2:850649/76‑1 (MQ=255) gaTCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgt < 2:2199827/75‑1 (MQ=255) aTCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtg < 2:1094976/76‑1 (MQ=255) aTCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtg > 1:3411596/1‑76 (MQ=255) aTCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtg < 1:4765751/76‑1 (MQ=255) tctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtggtg > 1:1672309/1‑76 (MQ=255) tctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtggtg > 1:4563865/1‑76 (MQ=255) tctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtggtg > 2:2218617/1‑76 (MQ=255) ctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGa > 1:4765289/1‑76 (MQ=255) ctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGa < 1:3918450/76‑1 (MQ=255) ctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGa > 2:2159088/1‑76 (MQ=255) ctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGa > 1:1261387/1‑76 (MQ=255) ccAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGtt < 2:888224/76‑1 (MQ=255) ccAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGtt > 1:968469/1‑76 (MQ=255) aaTACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTa > 2:715543/1‑76 (MQ=255) aaTACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTa < 1:2223812/76‑1 (MQ=255) aaTACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTa < 1:2199365/76‑1 (MQ=255) ggCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCg < 1:3675405/76‑1 (MQ=255) ggCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCg < 2:2407086/76‑1 (MQ=255) gCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg > 2:3502104/1‑76 (MQ=255) gCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg < 1:4080493/76‑1 (MQ=255) gCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg > 1:2323647/1‑76 (MQ=255) gCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg > 1:736728/1‑76 (MQ=255) cAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGt > 1:1634735/1‑76 (MQ=255) aaTGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTg < 1:2846874/76‑1 (MQ=255) aaTGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTg > 2:2969043/1‑76 (MQ=255) aTGCCACAGATATTTGCTCTCGCGCGGGAATAGGCCGATAACTCGGCGCGAGACGAGGCGATGTTTAGCTCGGTgg > 2:934892/1‑75 (MQ=255) tGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAt > 1:1206269/1‑76 (MQ=255) gCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtg < 1:2023947/76‑1 (MQ=255) ccgcaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgc < 1:4731126/73‑1 (MQ=255) ccACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgc < 2:3673743/76‑1 (MQ=255) ccACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgc < 1:1425891/76‑1 (MQ=255) cacaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGACGCGGCGATGTTTAGCTCGGTGAtgcg > 2:4717767/1‑76 (MQ=255) cacaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcg < 1:4378037/76‑1 (MQ=255) cacaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcg < 2:4086145/76‑1 (MQ=255) cacaGCTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcg > 1:2453050/1‑76 (MQ=255) acaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgt > 2:1531328/1‑76 (MQ=255) aGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgtgc < 2:4608803/76‑1 (MQ=255) gTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgtgcg < 1:4467767/76‑1 (MQ=255) gTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgtgcg < 1:1354268/76‑1 (MQ=255) ttATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTgcgc < 1:2975780/76‑1 (MQ=255) gtATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTgcgc < 1:295070/75‑1 (MQ=255) tATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCa < 1:4512842/76‑1 (MQ=255) aTTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAg > 2:3632995/1‑76 (MQ=255) tGCTCTGGGGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCtt < 2:71911/76‑1 (MQ=255) gCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCttt < 1:1998718/76‑1 (MQ=255) tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgc < 2:2099011/62‑1 (MQ=255) tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTtct > 2:4746628/1‑76 (MQ=255) tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTtct > 2:4400095/1‑76 (MQ=255) tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTtct < 1:4502361/76‑1 (MQ=255) | TGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGAGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCT > NC_000913/77931‑78073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |