BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,797	T→C	F128S (TTT→TCT)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,797	0	T	C	100.0%	303.9 / NA	113	F128S (TTT→TCT)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (64/49); total (64/49)

Only 100 of 113 total aligned reads displayed.

GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG  >  NC_000913/2824726‑2824867

ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTcc                                                                    >  2:1756978/1‑76 (MQ=255)

 gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACCACCCCCTTCCg                                                                   >  2:89080/1‑76 (MQ=255)

 gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                   >  2:3740766/1‑76 (MQ=255)

 gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                   <  2:659044/76‑1 (MQ=255)

     atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACAACACAACCCTCTTCCGTggg                                                               >  1:745509/1‑76 (MQ=255)

       atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                             <  1:4633039/76‑1 (MQ=255)

       atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                             <  2:3301026/76‑1 (MQ=255)

        tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCg                                                            >  2:1415459/1‑76 (MQ=255)

        attATGTCGCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCg                                                            <  2:448038/75‑1 (MQ=255)

         ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                           >  1:3378378/1‑76 (MQ=255)

         ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                           >  1:367082/1‑76 (MQ=255)

          tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                          >  1:1864401/1‑76 (MQ=255)

           aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                         <  2:4421563/76‑1 (MQ=255)

           aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                         <  2:2837798/76‑1 (MQ=255)

            tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                        >  1:3820025/1‑76 (MQ=255)

             gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                       <  1:351737/76‑1 (MQ=255)

             gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGCGACt                                                       >  1:4206232/1‑76 (MQ=255)

             gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCCTCCGTGGGCCGTGACt                                                       >  1:4220994/1‑76 (MQ=255)

              tctcTGGCGTAAAAGAAGAAAACATCGCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                      <  1:3870867/76‑1 (MQ=255)

              tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                      >  2:4297648/1‑76 (MQ=255)

              tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                      >  1:3759750/1‑76 (MQ=255)

              tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                      <  2:4764166/76‑1 (MQ=255)

              tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                      >  2:1885047/1‑76 (MQ=255)

              tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                      <  1:3380048/76‑1 (MQ=255)

              tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGAGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                      <  2:3973223/76‑1 (MQ=255)

               ttctGGGGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                     <  2:1403979/75‑1 (MQ=255)

               ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCCCAACCCTCTTCCGTGGGCCGTGACTAt                                                     <  1:3949206/76‑1 (MQ=255)

               ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                     <  1:2187877/76‑1 (MQ=255)

               ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                     >  2:4011345/1‑76 (MQ=255)

               ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                     >  1:628575/1‑76 (MQ=255)

               ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCGCTTCCGTGCGCCGTGTCTAt                                                     >  1:2797682/1‑76 (MQ=255)

                tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                    <  1:2895543/76‑1 (MQ=255)

                 ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                   >  2:1930861/1‑76 (MQ=255)

                    gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                <  2:3908938/76‑1 (MQ=255)

                    gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                <  1:745536/76‑1 (MQ=255)

                    gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                <  1:2821506/76‑1 (MQ=255)

                     cGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                               >  1:4219109/1‑76 (MQ=255)

                      gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                              >  1:3152787/1‑76 (MQ=255)

                      gTAAAAGAAGAAAACAGCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                              <  1:3378673/76‑1 (MQ=255)

                       tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCCTGACTACGACACCAg                                             >  1:224738/1‑76 (MQ=255)

                        aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAga                                            >  1:402623/1‑75 (MQ=255)

                        aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                            >  2:1400107/1‑76 (MQ=255)

                        aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                            <  1:4741785/76‑1 (MQ=255)

                        aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                            <  1:1225740/76‑1 (MQ=255)

                        aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCAGTCGGCCGTGACTATGACATCAgg                                            >  1:800107/1‑75 (MQ=255)

                         aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                           >  1:62804/1‑76 (MQ=255)

                          aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                          >  1:3070389/1‑76 (MQ=255)

                           agaagaAAACATCACTGTAGTAGGTGCTGCTACACCACAACCCTCTTCCGTGTGCCGTGGCTATGACACCAGTAAg                                         <  1:4177227/76‑1 (MQ=255)

                           agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                         >  2:3272407/1‑76 (MQ=255)

                           agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                         <  1:2454313/76‑1 (MQ=255)

                           agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                         >  2:639971/1‑76 (MQ=255)

                            gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                        >  2:4562070/1‑76 (MQ=255)

                            gaagaaAACATCACTGTAGCAGGTGATGCGACACCACAACCCTCTTCCGGGGGCCGCGACTATGACACCAGTAAGa                                        >  2:4553412/1‑76 (MQ=255)

                                 aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                   >  2:1419570/1‑76 (MQ=255)

                                  taCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                  >  2:2373200/2‑76 (MQ=255)

                                  aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                  >  1:3258460/1‑76 (MQ=255)

                                    cATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcc                                <  2:3488617/76‑1 (MQ=255)

                                    cATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcc                                <  1:1555333/76‑1 (MQ=255)

                                     aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                               <  2:935757/76‑1 (MQ=255)

                                      tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTCTGCCACCAGTAAGAAACTCACCgc                              >  2:3935187/1‑75 (MQ=255)

                                      tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCgc                              >  2:3898974/1‑75 (MQ=255)

                                       cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                             <  1:3378562/76‑1 (MQ=255)

                                       cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                             <  1:2254600/76‑1 (MQ=255)

                                       cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                             >  2:2471534/1‑76 (MQ=255)

                                       cACGGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                             <  2:442582/76‑1 (MQ=255)

                                          tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                          >  2:2724832/1‑76 (MQ=255)

                                          tGTAGTAGGTGATGCGACACCACAACCCTCTTACGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                          >  2:1796054/1‑76 (MQ=255)

                                               tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                     <  2:1191735/76‑1 (MQ=255)

                                               tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                     >  1:618113/1‑76 (MQ=255)

                                               tagGTGATGCGACACCACAACCCTCTTCCGTGCGCCGTGACTATGACACCAGTAAGAAAATCAACGAACAAAGCGa                     >  2:4522106/1‑76 (MQ=255)

                                                agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                    >  1:1896220/1‑76 (MQ=255)

                                                agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                    >  2:3410350/1‑76 (MQ=255)

                                                agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                    >  1:3111575/1‑76 (MQ=255)

                                                acgTGATGCGACACCTCTACCCACTTCCGTGTGCCGTGCCTATGACGCCAGTAAGAAAATCACCGAACAAAGCGAt                    <  1:4108416/74‑1 (MQ=255)

                                                 ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                   >  1:2464985/1‑76 (MQ=255)

                                                   tGATGCGACACCACAACCCTCTTCCGTGTGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                 >  2:2055882/1‑76 (MQ=255)

                                                   tGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                 >  1:3966764/1‑76 (MQ=255)

                                                   tGATGCGACACCACAACCCTCTTCCGTGGACCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                 >  1:4214316/1‑76 (MQ=255)

                                                   ggATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                 <  2:1291752/75‑1 (MQ=255)

                                                    gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                <  1:1380706/76‑1 (MQ=255)

                                                    gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                <  2:8546/76‑1 (MQ=255)

                                                     aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGCTGGttt               >  2:4256920/1‑76 (MQ=255)

                                                     aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt               >  1:4404020/1‑76 (MQ=255)

                                                     aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt               >  1:2575861/1‑76 (MQ=255)

                                                     aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt               <  1:2573092/76‑1 (MQ=255)

                                                      tGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa              >  2:2701771/1‑76 (MQ=255)

                                                         gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg           <  1:4475013/76‑1 (MQ=255)

                                                          acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg          <  2:3829374/76‑1 (MQ=255)

                                                          acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg          >  1:1033464/1‑76 (MQ=255)

                                                          acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg          >  1:3457210/1‑76 (MQ=255)

                                                          acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg          <  1:970786/76‑1 (MQ=255)

                                                           caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc         <  1:2811870/76‑1 (MQ=255)

                                                            accacAACCCTCTTGCGTGGGCCGTGACTACGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg        <  2:1229268/76‑1 (MQ=255)

                                                             ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGa       <  2:1896219/76‑1 (MQ=255)

                                                               acaACCCTCTTCAGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg     <  1:3190257/76‑1 (MQ=255)

                                                                 aaCCCTCTTCCGTGGGTCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt   <  1:2569625/76‑1 (MQ=255)

                                                                  aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAggcg  >  2:3765184/1‑74 (MQ=255)

                                                                  aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg  >  1:1280348/1‑76 (MQ=255)

                                                                  aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg  <  2:85123/76‑1 (MQ=255)

                                                                  aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg  >  1:1147025/1‑76 (MQ=255)

GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG  >  NC_000913/2824726‑2824867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`