BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,102	T→C	F230L (TTC→CTC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,102	0	T	C	100.0%	400.5 / NA	150	F230L (TTC→CTC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (83/67); total (83/67)

Only 100 of 152 total aligned reads displayed.

CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTC  >  NC_000913/2825030‑2825174

ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCg                                                                       >  2:2847970/1‑76 (MQ=255)

ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCg                                                                       <  1:3089759/76‑1 (MQ=255)

 cGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCgg                                                                      >  1:3875379/1‑76 (MQ=255)

    tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                   >  1:3177585/1‑76 (MQ=255)

     ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCCCTGCTTTCACCTTTCCTCGGcccc                                                                  >  2:4864549/1‑75 (MQ=255)

     ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                  >  1:3550233/1‑76 (MQ=255)

     ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                  >  1:1466162/1‑76 (MQ=255)

     ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                  <  1:518705/76‑1 (MQ=255)

      gCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                 >  2:4653089/1‑76 (MQ=255)

       cGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCGCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                >  2:632204/1‑76 (MQ=255)

       cGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                >  1:3633370/1‑76 (MQ=255)

       cGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                <  1:3828138/76‑1 (MQ=255)

        gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                               <  1:4441685/76‑1 (MQ=255)

        gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                               >  2:3111340/1‑76 (MQ=255)

        gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                               <  1:153419/76‑1 (MQ=255)

         ggCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                              <  2:1273862/75‑1 (MQ=255)

         aGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                              >  2:1233948/1‑76 (MQ=255)

         aGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                              >  2:385706/1‑76 (MQ=255)

          gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcgc                                                             <  2:3256820/76‑1 (MQ=255)

           ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                            >  1:386316/1‑76 (MQ=255)

           ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                            >  1:93027/1‑76 (MQ=255)

            cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                           <  1:3933254/76‑1 (MQ=255)

             aTCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGt                                                          >  1:1677891/1‑76 (MQ=255)

             aTCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGt                                                          <  1:1565143/76‑1 (MQ=255)

                cACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAt                                                       <  1:4392146/76‑1 (MQ=255)

                 aCTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                      <  2:3729476/76‑1 (MQ=255)

                 aCTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                      >  1:1236958/1‑76 (MQ=255)

                  cTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCg                                                     <  2:3075160/76‑1 (MQ=255)

                  cTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCg                                                     >  2:1986198/1‑76 (MQ=255)

                   tGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGc                                                    <  1:1748718/76‑1 (MQ=255)

                   tGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGc                                                    <  2:3477811/76‑1 (MQ=255)

                    gggTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                   >  2:1427380/1‑76 (MQ=255)

                    gggTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                   <  2:1146802/76‑1 (MQ=255)

                       tCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAg                                                <  1:3528828/76‑1 (MQ=255)

                        cTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAgg                                               <  1:436862/76‑1 (MQ=255)

                         tGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGt                                              >  2:2092264/1‑76 (MQ=255)

                          ggTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGtt                                             <  2:2861449/76‑1 (MQ=255)

                          ggTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGtt                                             >  2:2298783/1‑76 (MQ=255)

                            tCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                           >  2:2063381/1‑76 (MQ=255)

                             cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                          >  2:573400/1‑76 (MQ=255)

                             cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                          <  1:2320513/76‑1 (MQ=255)

                             cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                          <  2:2193439/76‑1 (MQ=255)

                             cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGGTATc                                          >  2:4556194/1‑76 (MQ=255)

                             cATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCCGCCCAGGCGCAGTTATCGCACAGGGTATc                                          >  1:2453120/1‑76 (MQ=255)

                             cATGCTGGCGCTCATCTGTNNNTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGAGCAGTTCTNGCACAGGATATc                                          >  1:3683256/1‑76 (MQ=255)

                              aTGCTGGCGCTCATCTGTTCCTTCGCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                         <  1:627385/76‑1 (MQ=255)

                              aTGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                         <  1:133756/76‑1 (MQ=255)

                               tGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                        <  1:573400/76‑1 (MQ=255)

                               tGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                        <  1:4772986/76‑1 (MQ=255)

                               tGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                        <  1:4769697/76‑1 (MQ=255)

                               tGCTGGCGCTCATCTGATCCTTCCCACTGCATTCACCTTTCCTAGCCCCAGGCGACGACATCGCACAGGCTATCgc                                        >  2:270054/1‑75 (MQ=255)

                                 cTGGCGCGCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCg                                      >  1:4818209/1‑76 (MQ=255)

                                  tGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGt                                     >  1:3244629/1‑76 (MQ=255)

                                  tGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGCTCTCGCACAGGCGATGGGCgg                                     >  1:42935/1‑75 (MQ=255)

                                   ggCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTa                                    >  1:4697754/1‑76 (MQ=255)

                                    gcgcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                   >  1:1173057/1‑76 (MQ=255)

                                    gcgcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                   >  1:2325357/1‑76 (MQ=255)

                                     cgcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTatt                                  >  1:2408834/1‑76 (MQ=255)

                                      gcTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattg                                 >  2:4044585/1‑76 (MQ=255)

                                       cTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattga                                <  1:2108267/76‑1 (MQ=255)

                                       cTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGCTATCACACAGGTTATCGGCGTagtga                                >  2:2596531/1‑76 (MQ=255)

                                          aTCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                             >  1:999668/1‑76 (MQ=255)

                                          aTCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                             >  2:1497887/1‑76 (MQ=255)

                                           tCTGTTCCTTCCCACTGCTTGCACCGTGCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTgg                            <  1:584508/76‑1 (MQ=255)

                                            cTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                           <  1:3359635/76‑1 (MQ=255)

                                             tGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCTCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCg                          <  1:1741117/76‑1 (MQ=255)

                                             tGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCg                          <  2:2241054/76‑1 (MQ=255)

                                              gTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                         <  2:4434274/76‑1 (MQ=255)

                                              gTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                         >  2:4397457/1‑76 (MQ=255)

                                               ttccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTg                        <  2:2403770/76‑1 (MQ=255)

                                               ttccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGCTATCGGCGTATTGCTTGGCgcg                        >  1:1298413/1‑74 (MQ=255)

                                               ttccatccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTg                        <  1:2755168/76‑1 (MQ=255)

                                                tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGc                       >  1:4708887/1‑76 (MQ=255)

                                                 ccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa                      >  1:1975909/1‑76 (MQ=255)

                                                  cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGTCGTGccg                     >  2:2708023/1‑74 (MQ=255)

                                                  cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAg                     >  1:380481/1‑76 (MQ=255)

                                                  cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAg                     <  1:2509239/76‑1 (MQ=255)

                                                   ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGa                    <  1:2393312/76‑1 (MQ=255)

                                                    tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                   >  1:4852091/1‑76 (MQ=255)

                                                    tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                   >  2:2576642/1‑76 (MQ=255)

                                                    tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                   >  1:753673/1‑76 (MQ=255)

                                                     cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                  >  1:719962/1‑76 (MQ=255)

                                                     cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                  >  2:1782235/1‑76 (MQ=255)

                                                     cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                  <  1:32914/76‑1 (MQ=255)

                                                     cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                  >  1:3923909/1‑76 (MQ=255)

                                                      ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg                 >  2:4426604/1‑76 (MQ=255)

                                                      ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg                 >  1:621276/1‑76 (MQ=255)

                                                        aCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCCGATTGGt               >  2:636389/1‑76 (MQ=255)

                                                          tGCTTTCACCTTTCCTCGGCCCAGGCGCAGTGATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtct             <  2:75538/76‑1 (MQ=255)

                                                            cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg           <  1:93649/76‑1 (MQ=255)

                                                            cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg           >  1:2853128/1‑76 (MQ=255)

                                                             tttCGCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCgg          <  2:15403/76‑1 (MQ=255)

                                                               tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCa        <  2:4716731/76‑1 (MQ=255)

                                                                cACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCac       >  2:4420241/1‑75 (MQ=255)

                                                                 aCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGCTTGGTCTCGGCact      >  2:1787550/1‑74 (MQ=255)

                                                                 aCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat      >  2:2521064/1‑76 (MQ=255)

                                                                  ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTACCGGCGTATTGATTGGCGTGCAGATTGGTCGCGCCCata     >  2:4108542/1‑76 (MQ=255)

                                                                    tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCTGATTGGTCTCGGCAATAtt   <  2:1976931/76‑1 (MQ=255)

                                                                    tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt   <  2:1219889/76‑1 (MQ=255)

                                                                     ttctgcGGCCGAGGCGCAGTTATCGCACAGGTTGTCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTc  <  2:4710519/76‑1 (MQ=255)

CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTC  >  NC_000913/2825030‑2825174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`