BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,905	A→G	S584P (TCG→CCG)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,905	0	A	G	99.4%	399.9 / NA	154	S584P (TCG→CCG)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (59/93); minor base T (0/1); total (59/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

GTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGACATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTC  >  NC_000913/3077833‑3077977

gTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCag                                                                       >  2:4324118/1‑75 (MQ=255)

gTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCAt                                                                       <  1:499269/76‑1 (MQ=255)

gTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCAt                                                                       >  2:4126007/1‑76 (MQ=255)

gTGCAATAACCGCTGTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCAt                                                                       <  2:1419667/76‑1 (MQ=255)

 tGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATg                                                                      <  2:3646280/76‑1 (MQ=255)

 tGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATg                                                                      <  1:629296/76‑1 (MQ=255)

   cAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGa                                                                    >  1:3932341/1‑76 (MQ=255)

     gTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGaca                                                                  >  1:2661313/1‑76 (MQ=255)

         ccGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                              <  1:4140161/76‑1 (MQ=255)

         ccGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                              <  1:1131010/76‑1 (MQ=255)

          cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcg                                                             >  1:3342281/1‑76 (MQ=255)

          cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcg                                                             <  1:4719957/76‑1 (MQ=255)

           gCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgc                                                            >  2:1498295/1‑76 (MQ=255)

            cTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca                                                               >  2:3330454/1‑72 (MQ=255)

            cTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcg                                                           >  2:3758662/1‑76 (MQ=255)

             tttCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc                                                          <  1:4352678/76‑1 (MQ=255)

              ttCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCAATGACAACAcgcgag                                                         >  1:4169175/1‑74 (MQ=255)

               tCGGCAGTACGGATTCACTGTCAGCAGCATCCTGCTTGTCAAACGCGTCGTAACACCACATGGACCCCAcgcgctc                                                        <  1:3300452/76‑3 (MQ=255)

               tCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACgcgcgc                                                        <  1:2322019/76‑1 (MQ=255)

               tCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACgcgcgc                                                        <  1:309639/76‑1 (MQ=255)

                 ggCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCCCgcgcgcct                                                      >  2:3882406/1‑74 (MQ=255)

                  gCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt                                                     >  2:3534917/1‑76 (MQ=255)

                   cAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTc                                                    >  2:3077493/1‑76 (MQ=255)

                   cAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTc                                                    >  2:1545470/1‑76 (MQ=255)

                   cAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTc                                                    >  1:1978127/1‑76 (MQ=255)

                    aGTGCGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCa                                                   <  2:2131849/76‑1 (MQ=255)

                    aGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCa                                                   <  1:3228840/76‑1 (MQ=255)

                    aGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCa                                                   <  1:3133338/76‑1 (MQ=255)

                    aGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCa                                                   <  2:1253184/76‑1 (MQ=255)

                    aGTACGGATTCACGGGAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGGCGGCATGGACACCACGCGCGCTTTCa                                                   >  1:3816486/1‑76 (MQ=255)

                     gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCAc                                                  <  2:1567441/76‑1 (MQ=255)

                     gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCAc                                                  <  1:196967/76‑1 (MQ=255)

                     gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCAc                                                  <  2:4718878/76‑1 (MQ=255)

                     gTACGGATTCACGGTAAGCAGCAGCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCAc                                                  <  2:576920/76‑1 (MQ=255)

                     gTACGGAGTCACGGTAAGCAGCATCCTGCTTGTCAAGTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTacac                                                  <  2:3409051/76‑1 (MQ=255)

                       aCGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGc                                                <  2:1851090/76‑1 (MQ=255)

                       aCGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGc                                                <  2:4714938/76‑1 (MQ=255)

                        cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGcc                                               <  2:2026726/76‑1 (MQ=255)

                        cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGcc                                               <  1:4063616/76‑1 (MQ=255)

                        cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCCCGCTCTCAcccc                                               >  2:692971/1‑76 (MQ=255)

                         ggATTCACGGTAAGTAGCATGCTGCTTGTCAAATGCGTCGGTAGACGGGATCGGCACCACGCGCGATGTCACGCCt                                              >  1:508822/1‑76 (MQ=255)

                         ggATCCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCt                                              <  1:1040076/76‑1 (MQ=255)

                           aTTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                            <  2:2992245/76‑1 (MQ=255)

                           aTTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                            <  1:2013010/76‑1 (MQ=255)

                           aTTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                            <  1:1269268/76‑1 (MQ=255)

                           aTTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                            <  1:2859511/76‑1 (MQ=255)

                           aTTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                            <  1:3853521/76‑1 (MQ=255)

                           aTTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCATTCACGCCTTc                                            <  1:3013796/76‑1 (MQ=255)

                           aTTCACGGTAAGCAGCATCCTCCATGTCACATGCGTACGTCGACGGCATGTACAGCACGCGCGGTTTCACGCCTTc                                            <  1:3874467/76‑1 (MQ=255)

                            ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCg                                           <  1:622691/76‑1 (MQ=255)

                            ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCg                                           <  1:968977/76‑1 (MQ=255)

                            ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCg                                           <  2:2098262/76‑1 (MQ=255)

                            ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCg                                           <  2:3818812/76‑1 (MQ=255)

                             tCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCgg                                          >  1:4758748/1‑76 (MQ=255)

                              cACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGc                                         <  1:2571606/76‑1 (MQ=255)

                               aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTGGACGGCAGGGACCCCCCGCGCGCTTCCCCGCCTTCGGcc                                        >  2:324389/1‑75 (MQ=255)

                               aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCCCGCGCGCTTTCACGCCTTCGGcg                                        >  1:3269006/1‑75 (MQ=255)

                               aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca                                        >  2:3264368/1‑76 (MQ=255)

                               aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca                                        <  1:3526697/76‑1 (MQ=255)

                                cGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGCAGGCCAGGACACCACGCGCGCTTTCACGCCTTAGGcac                                       >  1:923572/1‑75 (MQ=37)

                                cGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcag                                       <  1:770176/76‑1 (MQ=255)

                                 ggTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt                                      <  1:2381185/76‑1 (MQ=255)

                                  gTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCCCGCGCGCTTTCACGCCTTCGGcagtc                                     >  2:3990345/1‑76 (MQ=255)

                                   tAAGCAGCATCCTGCTTGTCAAATGCGTCGGTGGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtca                                    <  2:3234639/76‑1 (MQ=39)

                                   tAAGCAGCATCCTGCTGGTCAAAGGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtca                                    <  2:3786442/76‑1 (MQ=38)

                                    aaGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag                                   >  1:4743148/1‑76 (MQ=255)

                                       cagcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttt                                >  2:2914289/1‑76 (MQ=255)

                                         gcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACCCCCCCCTCCACCCCTTCGGCAGCCAGctttc                              >  2:18894/1‑75 (MQ=255)

                                          cacccTGCTTGTCAAATGCGCCGGTAGACGGCATGGACACCCCGCGCGCTTTCCCGCCTTCGGCAGTCCGTTTTTc                             <  1:4302002/73‑1 (MQ=255)

                                           aTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCg                            <  1:2994813/76‑1 (MQ=255)

                                           aTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCg                            >  1:1512588/1‑76 (MQ=255)

                                             ccTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCCCGCCTTCGGCAGGCAGTTTTTCGTa                          >  2:255775/1‑76 (MQ=255)

                                             ccTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTa                          >  1:3927870/1‑76 (MQ=255)

                                             ccTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGCTTTTCGtg                          >  1:2944728/1‑75 (MQ=39)

                                              ctgctgGTCAAATGCGGCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                         <  2:4459034/76‑1 (MQ=38)

                                              cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                         <  1:1691356/76‑1 (MQ=255)

                                              cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                         <  1:1020134/76‑1 (MQ=255)

                                              cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                         <  1:335497/76‑1 (MQ=255)

                                              cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                         <  1:3783776/76‑1 (MQ=255)

                                              cTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                         <  1:3584757/76‑1 (MQ=255)

                                                gcgtGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgc                       <  2:466276/73‑1 (MQ=255)

                                                 cTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgca                      <  2:1561695/76‑1 (MQ=255)

                                                  ttGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcag                     <  2:542930/76‑1 (MQ=255)

                                                   tGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgccgc                    >  2:1651789/1‑76 (MQ=255)

                                                     tCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCCGTCCGTTTTTCGTAGGCACCaa                  >  2:3996169/1‑76 (MQ=38)

                                                      cAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGGAGGCCGTTTTTTGTAGGCAGGAAc                 >  1:467863/1‑76 (MQ=25)

                                                      cAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAAc                 <  1:4085576/76‑1 (MQ=255)

                                                      cAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAAc                 <  1:2723569/76‑1 (MQ=255)

                                                       aaaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACa                <  2:4663738/76‑1 (MQ=255)

                                                       aaaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACa                <  1:1252425/76‑1 (MQ=255)

                                                       aaaTGCGTCGGCAGACGGCATGGCCACCACGCGCGCTTTCACGCCTTCGGCATGCCGTTTTTCGTAGGCAGCCAct                >  2:2432799/1‑75 (MQ=25)

                                                        aaTGCGTCGGTAGCCGGCATGGACACCGCGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAg               <  1:4328080/76‑1 (MQ=255)

                                                        aaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAg               <  2:2324413/76‑1 (MQ=255)

                                                        aaTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAg               <  1:2603127/76‑1 (MQ=255)

                                                            cGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAg           <  2:1303134/76‑1 (MQ=255)

                                                            cGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAg           <  1:2548649/76‑1 (MQ=255)

                                                             gTCGGTAGACGGCATGGACAACACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGcccat          >  1:3794452/1‑73 (MQ=38)

                                                                ggTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCa       >  1:1661065/1‑76 (MQ=255)

                                                                ggTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCa       >  1:4686605/1‑76 (MQ=255)

                                                                 gTAGACGGCATGGACACCCCGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCaa      <  2:2569298/76‑1 (MQ=255)

GTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGACATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTC  >  NC_000913/3077833‑3077977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`