BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,245	C→G	G470G (GGG→GGC)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,245	0	C	G	100.0%	504.0 / NA	194	G470G (GGG→GGC)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (108/86); total (108/86)

Only 100 of 196 total aligned reads displayed.

CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCA  >  NC_000913/3078172‑3078315

  cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGt                                                                    <  2:3450326/76‑1 (MQ=255)

   acGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                                   >  2:3461635/1‑76 (MQ=255)

     ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                 <  1:3659289/76‑1 (MQ=255)

     ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                                 >  2:1992830/1‑76 (MQ=255)

      gACGCCCTGTAGACATGTTCGGGGTTGCGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                <  2:2650701/76‑1 (MQ=255)

      gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                <  1:3689954/76‑1 (MQ=255)

      gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                >  2:1862698/1‑76 (MQ=255)

      gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                <  1:4001979/76‑1 (MQ=255)

       gcGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCg                                                               <  2:1235644/75‑1 (MQ=255)

       aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCg                                                               >  1:267438/1‑76 (MQ=255)

         gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGGCTTCGcc                                                             >  2:1633849/1‑76 (MQ=255)

          cgaTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                            <  1:4693792/74‑1 (MQ=255)

          ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                            <  1:1641/76‑1 (MQ=255)

           cATGTAGGCATGTTCGGGGTTACGAGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                           <  2:2461020/76‑1 (MQ=255)

           cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                           <  2:659113/76‑1 (MQ=255)

           cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                           >  2:3917123/1‑76 (MQ=255)

           cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCCTCTTCGcccc                                                           >  2:747984/1‑75 (MQ=255)

            aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                          >  1:1792185/1‑76 (MQ=255)

            aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                          >  1:1435380/1‑76 (MQ=255)

            aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                          >  1:1530062/1‑76 (MQ=255)

            aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                          >  1:1766669/1‑76 (MQ=255)

            aTGTAGACATGTTCGGGGGTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccccg                                                          >  1:4841831/1‑74 (MQ=255)

             tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccccgc                                                         >  2:4392926/1‑73 (MQ=255)

             tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCCGa                                                         >  2:4835747/1‑76 (MQ=255)

             tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                         <  2:1237363/76‑1 (MQ=255)

               tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                       >  1:1391672/1‑76 (MQ=255)

                aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCg                                                      >  1:988396/1‑76 (MQ=255)

                aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCg                                                      >  1:1277251/1‑76 (MQ=255)

                  gcATGTGCGGGGGGACGCGCAGAGAAGCGACCTGCTCAAGCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                    <  2:25600/75‑1 (MQ=255)

                   cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                   <  1:2695437/76‑1 (MQ=255)

                    aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATgg                                                  >  1:1143757/1‑76 (MQ=255)

                     tgtgCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                                 <  1:3868303/76‑1 (MQ=255)

                      gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAg                                                >  1:3206703/1‑76 (MQ=255)

                       ttCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCCTCGCCCCGACCGATGGAgc                                               >  2:1167820/1‑75 (MQ=255)

                       ttCGGGGTGACGCGCGGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                               <  2:3224513/76‑1 (MQ=255)

                        tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                              <  2:400139/76‑1 (MQ=255)

                        tCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                              <  2:4314769/76‑1 (MQ=255)

                        tCGGGGTGACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                              <  2:1551973/76‑1 (MQ=255)

                        gcGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                              <  2:4844505/75‑1 (MQ=255)

                           gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                           <  1:575430/76‑1 (MQ=255)

                           gagTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                           <  1:1590187/74‑1 (MQ=255)

                              ttACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGCGAGTCGGGCCGTCTTCGCCCACACCGATGCAGCCGTgcgg                                        >  2:994573/1‑73 (MQ=255)

                               tACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtg                                       >  1:2305810/1‑76 (MQ=255)

                               tACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtg                                       <  2:4077739/76‑1 (MQ=255)

                               tACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtg                                       >  1:4110226/1‑76 (MQ=255)

                                aCGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                      <  2:2173780/76‑1 (MQ=255)

                                 cgcgCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                     <  2:4677886/76‑1 (MQ=255)

                                  gcgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTaa                                    >  2:1753093/1‑76 (MQ=255)

                                   cgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                                   <  1:2210908/76‑1 (MQ=255)

                                   cgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                                   <  2:4217206/76‑1 (MQ=255)

                                    gcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                                  >  2:2722919/1‑76 (MQ=255)

                                    gcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCACCGATCGACGGAGTGGTGGGTGTGAAc                                  >  1:4281645/1‑76 (MQ=255)

                                     cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                 <  1:902992/76‑1 (MQ=255)

                                      agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                >  1:3797259/1‑76 (MQ=255)

                                      agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                >  2:3470570/1‑76 (MQ=255)

                                        agaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTTTAAACCATc                              <  1:3446570/76‑1 (MQ=255)

                                        agaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATc                              <  1:2870532/76‑1 (MQ=255)

                                         gaAGCGCCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCATACCGATGGAGTCGTGGGTGTAAACCATCa                             <  2:2410118/76‑1 (MQ=255)

                                         gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCa                             <  2:1542267/76‑1 (MQ=255)

                                         gaAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCa                             <  2:2337745/76‑1 (MQ=255)

                                          aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAc                            <  2:2852472/76‑1 (MQ=255)

                                          aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGCGGGTGTAAACcaccac                            >  2:4440397/1‑76 (MQ=255)

                                            gCGGCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                          <  2:523284/76‑1 (MQ=255)

                                            gCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTACGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                          <  2:80043/76‑1 (MQ=255)

                                             cGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAccag                         >  1:190873/1‑74 (MQ=255)

                                             cGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTg                         >  1:3341867/1‑76 (MQ=255)

                                              gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCtca                        >  2:580606/1‑74 (MQ=255)

                                              gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                        >  1:3848183/1‑76 (MQ=255)

                                               aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                       <  1:2039744/76‑1 (MQ=255)

                                               aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                       >  1:4092891/1‑76 (MQ=255)

                                               aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTCAc                       >  2:2346765/1‑76 (MQ=255)

                                                ccggCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACg                      <  2:3092478/73‑1 (MQ=255)

                                                 cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTCAACCATCACCTGACGc                     <  1:2261500/76‑1 (MQ=255)

                                                 cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGc                     >  1:1882373/1‑76 (MQ=255)

                                                  tGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCt                    <  1:1613133/76‑1 (MQ=255)

                                                  tGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAACCATCACCTGACGCt                    >  1:1201737/1‑76 (MQ=255)

                                                   gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTg                   >  2:2942398/1‑76 (MQ=255)

                                                   gCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTg                   <  1:3632729/76‑1 (MQ=255)

                                                     tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTggt                 >  1:4747886/1‑74 (MQ=255)

                                                     tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt                 <  1:2091835/76‑1 (MQ=255)

                                                     tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGCCGTGGGTGTAAACCATCACCTGACGCTgct                 >  1:4267059/1‑74 (MQ=255)

                                                      cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt                >  1:4818324/1‑76 (MQ=255)

                                                        aCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca              >  2:3695309/1‑76 (MQ=255)

                                                        aCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTCACGCTGTTtca              >  2:3062998/1‑76 (MQ=255)

                                                         ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcac             >  2:3612714/1‑75 (MQ=255)

                                                          gtgCTGGTGAGTCGTGCGGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc            <  2:2121904/74‑1 (MQ=255)

                                                          cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc            <  1:1990263/76‑1 (MQ=255)

                                                           ggCTGGTGAGTCGGGCCTTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca           <  1:662743/76‑1 (MQ=255)

                                                            gCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAg          >  1:4356888/1‑76 (MQ=255)

                                                             gtGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc         <  2:964825/75‑1 (MQ=255)

                                                             cTGGTGAGTCGGGCCGTCGTCGCACAGAACGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc         <  2:1919218/76‑1 (MQ=255)

                                                               ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc       >  1:2611400/1‑76 (MQ=255)

                                                               ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc       >  1:753836/1‑76 (MQ=255)

                                                               ggTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGCGTAAACCATCCCCCCACGCCCTTTCCCCAgccc       >  2:4621648/1‑74 (MQ=255)

                                                               ggTGAGTCGGGCCGTCGTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc       <  2:1435379/76‑1 (MQ=255)

                                                                gTGAGTGGGGCCGTCTGCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa      <  2:354934/76‑1 (MQ=255)

                                                                gTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa      <  2:2901483/76‑1 (MQ=255)

                                                                 tGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgccg     >  2:2310041/1‑74 (MQ=255)

                                                                  gAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc    >  1:2917124/1‑76 (MQ=255)

                                                                  gAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGc    >  1:938981/1‑76 (MQ=255)

CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCA  >  NC_000913/3078172‑3078315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`