| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,279,197 | A→C | R145S (AGA→AGC) | agaA → | putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,279,197 | 0 | A | C | 99.3% | 353.1 / NA | 136 | R145S (AGA→AGC) | agaA | putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (57/77); total (58/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 137 total aligned reads displayed.
GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATA > NC_000913/3279125‑3279268 | tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCg < 1:4814918/76‑1 (MQ=255) cGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTAGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt < 1:3755498/76‑1 (MQ=255) gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt > 1:2540/1‑76 (MQ=255) gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt > 1:3305460/1‑76 (MQ=255) cTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTg < 1:2604821/76‑1 (MQ=255) cTGCAGCCGGCGCGAATGCTGTGTGTTGATGGTGTTCTGGGATCGCTTAAACAGGGCAAACGCGCCAGCGTCGTTg < 1:2609335/76‑1 (MQ=255) tGCATCCGGCGCGAATGCTGGTTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc < 1:1800969/76‑1 (MQ=255) aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 1:1813749/76‑1 (MQ=255) aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 2:1891114/76‑1 (MQ=255) aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt > 2:1521195/1‑76 (MQ=255) aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 1:4156425/76‑1 (MQ=255) aTCCGGCGCGAATGCTGGGTGTTGATGATGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt > 2:2175281/1‑76 (MQ=255) tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTg < 2:406074/76‑1 (MQ=255) tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTg > 1:4060027/1‑76 (MQ=255) ccGGCGCGAATGCTGGGTGTTGACGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg > 1:922380/1‑76 (MQ=255) cGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa < 1:4802330/76‑1 (MQ=255) cGGCGCGAATGCTGGGCGTTGATGGTGTTCTGGGATCGCGTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa > 1:4471962/1‑76 (MQ=255) gcgcGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa < 2:4696279/76‑1 (MQ=255) gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAgg > 1:298707/1‑75 (MQ=255) gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc < 1:618533/76‑1 (MQ=255) gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc > 2:3051057/1‑76 (MQ=255) gcgAAGGCGGGGAGTTGATGGAGTTCGGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTGGAGCTGGATaac > 2:3631847/1‑74 (MQ=255) gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 1:2901759/76‑1 (MQ=255) gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 1:2168611/76‑1 (MQ=255) gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 1:1692866/76‑1 (MQ=255) aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg < 1:88952/76‑1 (MQ=255) aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg < 1:385505/76‑1 (MQ=255) aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc < 1:4831579/76‑1 (MQ=255) tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCt < 1:1240600/76‑1 (MQ=255) gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg < 2:3641495/73‑1 (MQ=255) gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 1:345859/1‑76 (MQ=255) gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa < 2:4529809/76‑1 (MQ=255) gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 1:4739/1‑76 (MQ=255) cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc < 1:2921697/76‑1 (MQ=255) tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCcaca > 2:649851/1‑76 (MQ=255) gggTGTTGATGGTGTTCTGGGATCGCTCAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt < 2:1646244/76‑1 (MQ=255) ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg > 1:3003404/1‑76 (MQ=255) ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg < 1:729146/76‑1 (MQ=255) gtgtTGCTGGCGTTCTGGGATCGCTTAAACCGGGCAAACGCGCAAGAGGCGTGGCGCGGGTTAGCGGGTTGCAtgt > 1:4738228/1‑76 (MQ=255) gtgtTAATGATGTTCTGGAATCGCTTCGCCCGGTGAAGCGGTCGAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt < 1:2102190/76‑1 (MQ=255) ggtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgtg < 2:1913503/75‑1 (MQ=255) gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGc > 2:3984158/1‑76 (MQ=255) ttGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATGGGGGGCCACTTGTGca > 2:4838946/1‑76 (MQ=255) ttGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGca < 1:2171133/76‑1 (MQ=255) gtGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGca < 1:469795/75‑1 (MQ=255) gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac > 1:1702613/1‑76 (MQ=255) gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac < 2:561842/76‑1 (MQ=255) gtGGTGTTCTGGGGTCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca < 2:1476898/75‑1 (MQ=255) aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg < 1:4615106/58‑1 (MQ=255) gggTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaacaa < 2:4042796/75‑1 (MQ=255) ggTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa > 1:4732284/1‑76 (MQ=255) gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt < 1:163841/76‑1 (MQ=255) gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt < 1:3161566/76‑1 (MQ=255) gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt < 1:4651526/76‑1 (MQ=255) gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt > 2:3808621/1‑76 (MQ=255) ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg < 1:2945014/76‑1 (MQ=255) tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATCGCGGGCTACATGTGCAACAAATCTgg > 2:4329370/1‑76 (MQ=255) tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg > 1:2890488/1‑76 (MQ=255) tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg > 2:4324823/1‑76 (MQ=255) cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa > 2:4577257/1‑76 (MQ=255) tGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAt < 1:3708584/76‑1 (MQ=255) gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt < 1:4682478/76‑1 (MQ=255) gggATCGCTGAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt < 2:148686/76‑1 (MQ=255) gATCGCTTAAACCGGGGAGACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 1:2366533/76‑1 (MQ=255) gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTcg > 2:1840613/1‑75 (MQ=255) gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa > 1:943695/1‑76 (MQ=255) gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 2:881403/76‑1 (MQ=255) gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 1:775848/76‑1 (MQ=255) gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa > 2:2879016/1‑76 (MQ=255) cGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg > 1:4323990/1‑76 (MQ=255) gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt < 1:2870376/76‑1 (MQ=255) gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt < 1:4146055/76‑1 (MQ=255) ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa > 2:3017932/1‑76 (MQ=255) ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 1:670321/76‑1 (MQ=255) ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 2:2146969/76‑1 (MQ=255) tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCaa > 2:1994824/1‑76 (MQ=255) aaaCCGGGCAAACGCGCCAGCTGCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAt < 2:3539728/76‑1 (MQ=255) aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAGTCTGGATTCAGGGTCAAt > 1:1393103/1‑76 (MQ=255) aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAt < 2:3496543/76‑1 (MQ=255) aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGCGCAACAAATCTGGATTCAGGGTCAAt > 2:1620250/1‑76 (MQ=255) cgcGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa < 2:2762580/74‑1 (MQ=255) ccGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg < 1:1069047/76‑1 (MQ=255) ccGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg < 1:2749891/76‑1 (MQ=255) gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt < 1:2629859/76‑1 (MQ=255) gggAAAGCGCGCGAGGGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt < 2:5184/76‑1 (MQ=255) ggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTTGCtt > 2:904248/1‑76 (MQ=255) gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc < 2:3905167/76‑1 (MQ=255) cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg > 1:1738151/1‑76 (MQ=255) aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt > 2:1891261/1‑76 (MQ=255) aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt > 2:3112095/1‑76 (MQ=255) aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt < 1:2101681/76‑1 (MQ=255) gcgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTg > 1:3451732/1‑76 (MQ=255) cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTgg > 2:4451310/1‑75 (MQ=255) cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGa < 2:3118772/76‑1 (MQ=255) gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt < 1:4590115/76‑1 (MQ=255) gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt > 1:392712/1‑76 (MQ=255) gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt > 1:3885883/1‑76 (MQ=255) ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa > 1:4318303/1‑76 (MQ=255) ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa < 2:4666305/76‑1 (MQ=255) ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa > 2:1104091/1‑76 (MQ=255) | GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATA > NC_000913/3279125‑3279268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |