| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,548,005 | C→T | G701D (GGT→GAT) | malP ← | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,548,005 | 0 | C | T | 98.4% | 370.3 / ‑6.5 | 121 | G701D (GGT→GAT) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (58/61); minor base G (2/0); total (60/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 134 total aligned reads displayed.
TCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAA > NC_000913/3547933‑3548077 | tCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCaccttatctt > 1:4109857/1‑76 (MQ=255) cgcaGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCttt < 2:1810703/73‑1 (MQ=255) cGAAGGCATGCGTATCGCCGGCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCttt > 1:4374679/1‑76 (MQ=255) gAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttc > 1:356855/1‑76 (MQ=255) agggCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttct < 2:2529271/74‑1 (MQ=255) aaGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttct < 1:1208418/76‑1 (MQ=255) aaGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttct < 2:4030774/76‑1 (MQ=255) aaGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttct < 1:3863092/76‑1 (MQ=255) aGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTAtctttctt > 2:795244/1‑76 (MQ=255) ggCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttcttc > 2:134254/1‑76 (MQ=255) gCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttcttca < 2:2569987/76‑2 (MQ=255) gCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTcc < 1:2330669/76‑1 (MQ=255) cATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCg < 1:4573492/76‑1 (MQ=255) aTGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGc > 1:694770/1‑76 (MQ=255) aTGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGc < 1:249099/76‑1 (MQ=255) aTGCTTATCGCCGTCGCTGTATTTACCCCTTTCCAGCTCTTTCAAGACTTCGGCCAGCACCTTATCTTTCTTCCGc > 2:1596558/1‑76 (MQ=255) tGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGcc < 1:1288980/76‑1 (MQ=255) ggCTTAGCGCCGTCGCTGTATGTACCGATTTCCAGCTCTGTCAATACGCCGTCCAGCACCTTATCTTTCTTCCgac > 1:3867341/2‑74 (MQ=255) gCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCa < 1:3330658/76‑1 (MQ=255) gCTTAGCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCGATACTGCGTCCAGCACCTTATCTTTCTTCCGCCa < 2:181346/76‑1 (MQ=255) gCTGATCGCCGTCGCTGCATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCa > 1:1192290/1‑76 (MQ=255) ttATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCAtt < 1:2522245/76‑1 (MQ=255) tATCGCCGTCTCTGTATTTGCCGCTTTCCAGCTCTTTCAATACTGCGTCCGGCACCTTCTCTTTCTTCCGCCAttt < 1:4159957/76‑1 (MQ=255) aTCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTc > 2:2407822/1‑76 (MQ=255) aTCGCCGTCGCTGTATTTACCGCGTTCGAGCGCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTc > 1:3471176/1‑76 (MQ=255) tCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCa > 2:2702312/1‑76 (MQ=255) gCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCGAGCACCTTATCTTTCTTCCGCCATTTCAcc < 1:4829386/76‑1 (MQ=255) gCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAcc < 1:2859094/76‑1 (MQ=255) cGTCGCTGTCTTTCCCGCTTTCCAGCTCTTTCACTCCGGGGTCCAGCTCCTTATCTTTCTTCCGCCATTTCGCCgg < 2:3458829/76‑1 (MQ=255) cGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCgg < 1:3292814/76‑1 (MQ=255) cGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCgg > 1:1780525/1‑76 (MQ=255) cGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCgg < 1:4565009/76‑1 (MQ=255) gTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCggg > 1:3110702/1‑76 (MQ=255) cGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCTCCGGGTc < 2:3000453/76‑1 (MQ=255) cGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTc < 2:4747910/76‑1 (MQ=255) gCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCg < 2:3734581/76‑1 (MQ=255) gCTGTATTTACCGCTGTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCg < 2:3097592/76‑1 (MQ=255) ccgTATTTACCGCTTTCCAGCGCTGTCAATACTGCGTCCAGCAACTGATCTTTCTTCCGCCATTTCACCGGGTCGt > 2:3197343/3‑76 (MQ=255) cTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGt < 2:854212/76‑1 (MQ=255) tGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCCCCTTATCTTTCTTCCGCCATTTCACCTGGTCGTa > 2:937083/1‑76 (MQ=255) tGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTa < 1:4118973/76‑1 (MQ=255) gTATTGACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAg < 2:4627906/76‑1 (MQ=255) tATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGc > 2:197372/1‑76 (MQ=255) tATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGc > 1:1864252/1‑76 (MQ=255) tttACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCt > 2:3988466/1‑76 (MQ=255) tttACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCt < 1:2236257/76‑1 (MQ=255) tACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCttt > 2:2772174/1‑76 (MQ=255) tACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCttt > 2:4846451/1‑76 (MQ=255) aCCGCTTTCCAGCTCTTTCTATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTg > 1:3638517/1‑76 (MQ=255) aCCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTg < 1:773821/76‑1 (MQ=255) aCCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTg > 1:3600812/1‑76 (MQ=255) ccGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTgg < 1:2307926/76‑1 (MQ=255) ccGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTgg < 2:3126858/76‑1 (MQ=255) cGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGc < 2:2011102/76‑1 (MQ=255) gCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCCCCGGGCCGCAGCCTTTCGcc > 2:4865192/1‑76 (MQ=255) gCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGcc > 1:4480571/1‑76 (MQ=255) cTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCa > 1:4028055/1‑76 (MQ=255) tttCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAg > 1:25685/1‑76 (MQ=255) cttCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAg < 1:287805/75‑1 (MQ=255) ttCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGa > 2:2858267/1‑76 (MQ=255) ccAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAAt > 1:133505/1‑76 (MQ=255) cAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAAtt < 2:4270553/76‑1 (MQ=255) cAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAAtt < 2:204230/76‑1 (MQ=255) aGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTg > 1:2380844/1‑76 (MQ=255) gCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGc > 1:4240638/1‑76 (MQ=255) tttCCATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTAACCGGGTCGTCGCCTTTGGCAAGACTAGCCtta > 2:1550201/1‑75 (MQ=255) tttCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTc > 2:1515640/1‑76 (MQ=255) ttCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCa > 2:3374336/1‑76 (MQ=255) ttCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCa > 2:248214/1‑76 (MQ=255) tCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAc > 1:719126/1‑76 (MQ=255) tCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAc < 2:2743180/76‑1 (MQ=255) tCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAc > 2:1359589/1‑76 (MQ=255) cAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACt < 1:3352949/76‑1 (MQ=255) cgaCTGCGACCAGCCCCCTCGCTTTCTTCCGCCATTTCACCGAGACGACTCCTTTGGCCAGCATCGCCCTCACTTg > 2:113882/3‑76 (MQ=255) aTACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTg < 1:2168118/76‑1 (MQ=255) aTACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTg > 2:121662/1‑76 (MQ=255) tACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGt < 2:1584407/76‑1 (MQ=255) aCTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGtt > 1:1760744/1‑76 (MQ=255) aCTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGtt > 1:2730363/1‑76 (MQ=255) cTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTc > 2:689750/1‑76 (MQ=255) tGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTcc > 2:4188862/1‑76 (MQ=255) cGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAc < 1:2227163/76‑1 (MQ=255) cGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAc > 2:457641/1‑76 (MQ=255) cGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAc > 1:4279689/1‑76 (MQ=255) cGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAc < 2:4109857/76‑1 (MQ=255) gTCCAGCTCCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACg < 2:673079/76‑1 (MQ=255) gTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACg > 2:2665106/1‑76 (MQ=255) gTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACg < 2:1687624/76‑1 (MQ=255) tCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACgg < 1:1359590/76‑1 (MQ=255) cAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTa < 1:3085737/76‑1 (MQ=255) cAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTa < 1:4846451/76‑1 (MQ=255) aGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTAt < 1:500127/76‑1 (MQ=255) gCACGTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATg < 1:3334937/76‑1 (MQ=255) cACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGa < 2:3173989/76‑1 (MQ=255) accttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAc > 1:2534899/1‑76 (MQ=255) ccttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAcc > 1:1208190/1‑76 (MQ=255) ccttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAcc > 2:2987090/1‑76 (MQ=255) cttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCa < 1:3384715/76‑1 (MQ=255) ttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCGTTCACTTGTTCCACGGTATGACCaa < 2:2753898/76‑1 (MQ=255) ttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCaa < 2:499611/76‑1 (MQ=255) | TCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAA > NC_000913/3547933‑3548077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |