| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,714,436 | 2 bp→GC | coding (261‑262/660 nt) | yiaD → | putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,714,436 | 0 | C | G | 99.0% | 255.4 / ‑4.9 | 100 | N87K (AAC→AAG) | yiaD | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (52/47); minor base A (1/0); total (53/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
| * | NC_000913 | 3,714,437 | 0 | G | C | 98.0% | 244.4 / NA | 100 | V88L (GTG→CTG) | yiaD | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base C (50/47); total (53/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT > NC_000913/3714364‑3714504 || gcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGGAGGGAGCGGAGGTg > 1:1621173/1‑76 (MQ=255) tGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGGTATTACATGGATGTGGGGGAAGCGAAGGTggg > 1:279891/1‑74 (MQ=255) gATTGGCGCAGCAGCAGGCGCGGCGCTGGGCGGCGGCGTTGGGTATTACATGGCTGGCCAGGAAGCGAAGCTgcgc > 2:1632178/1‑76 (MQ=255) gATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTgcgc > 2:2483328/1‑76 (MQ=255) aTTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGcgcg > 1:1708381/1‑76 (MQ=255) ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGa < 1:1148667/76‑1 (MQ=255) ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGa < 1:2642542/76‑1 (MQ=255) gtcgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGCAGCTGCGCGACa < 1:3872364/74‑1 (MQ=255) gcgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCCGGAAGCGCAGCTGCGCGACaa > 2:1976061/1‑76 (MQ=255) gcgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaa < 1:3231701/76‑1 (MQ=255) cgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCCCGACaaa > 2:3355854/1‑76 (MQ=255) cannngcagGCGCAGCTCTGNGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGANGCTGCGCGACAAAAt < 1:4597361/76‑1 (MQ=255) cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt < 1:3655119/76‑1 (MQ=255) cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt < 1:3227243/76‑1 (MQ=255) cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt < 1:802827/76‑1 (MQ=255) cagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg > 1:971693/1‑76 (MQ=255) cagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg < 1:4718943/76‑1 (MQ=255) agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc < 1:518284/76‑1 (MQ=255) agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc > 1:504773/1‑76 (MQ=255) agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc > 1:1045751/1‑76 (MQ=255) gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg > 1:2146939/1‑76 (MQ=255) gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCCGCGCGACAAAATGcgcg > 2:4127507/1‑76 (MQ=255) cagGCGCGGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg < 2:1318732/76‑1 (MQ=255) cagGCGCATCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg < 2:1362809/76‑1 (MQ=255) agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc > 1:602042/1‑76 (MQ=255) agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc > 1:2111598/1‑76 (MQ=255) ggCGCAGCTCGGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCa < 2:4310551/76‑1 (MQ=255) gcgcCGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAc < 2:3597544/76‑1 (MQ=255) cgcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAACGCGCGGCAcc > 2:2994882/1‑75 (MQ=255) gcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg < 1:1598701/76‑1 (MQ=255) cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTgg < 1:2314153/76‑1 (MQ=255) aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt > 1:2487342/1‑76 (MQ=255) gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACCAAATGCGCGGCACTCgtg > 2:136013/1‑76 (MQ=255) ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGGGCGGGCCTGgggt > 2:21068/1‑76 (MQ=255) ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtgt > 1:3878886/1‑76 (MQ=255) tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGtt < 1:2786053/76‑1 (MQ=255) ctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTa < 2:2649808/76‑1 (MQ=255) ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCg > 2:511980/1‑76 (MQ=255) ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCg > 1:1811643/1‑76 (MQ=255) ggcggcggcGTTGGGTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAGGCGCGGCAGTGGTGTTAGCg > 1:4677147/1‑76 (MQ=255) ggcggcggcGTTGATTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCg < 1:2169786/76‑1 (MQ=255) gggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa < 1:276221/75‑1 (MQ=255) cggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCgaa < 1:3474679/76‑3 (MQ=255) cggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTAGTGTTAGCGTa > 1:3440157/1‑76 (MQ=255) ggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTaa > 1:2919628/1‑76 (MQ=255) gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAc > 2:2858457/1‑76 (MQ=255) cggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc > 1:2407821/1‑76 (MQ=255) cggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc < 1:2468110/76‑1 (MQ=255) ggggTTGGGTATTACATGGAGGGGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc < 2:4750353/73‑1 (MQ=255) ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc < 1:2330262/76‑1 (MQ=255) ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc > 2:1793193/1‑76 (MQ=255) ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc < 1:160993/76‑1 (MQ=255) ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAccc > 1:2037019/1‑76 (MQ=255) gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCg > 2:2801900/1‑76 (MQ=255) gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCg > 2:3324205/1‑76 (MQ=255) cgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGc > 2:1775855/1‑76 (MQ=255) gTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa > 1:1077356/1‑76 (MQ=255) gTTGGTTATTACATGGATGTGCAGGAAGCGAAACTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa > 1:3106942/1‑76 (MQ=255) gTTGGTTAGTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa < 2:4844600/76‑1 (MQ=255) ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg > 1:3329981/1‑76 (MQ=255) tGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc < 1:21068/76‑1 (MQ=255) ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg > 1:3445747/1‑76 (MQ=255) gTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg < 2:2002190/76‑1 (MQ=255) ttattaCATGGATGGGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCggg < 2:447471/76‑1 (MQ=255) tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg > 2:2481056/1‑76 (MQ=255) attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa < 1:3137199/76‑1 (MQ=255) attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa > 2:1486695/1‑76 (MQ=255) attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa < 1:3525348/76‑1 (MQ=255) attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa < 2:525288/76‑1 (MQ=255) ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt > 1:36423/1‑76 (MQ=255) taCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATa > 1:4408050/1‑76 (MQ=255) cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAGCCCGCAGCGGGGATAAc < 1:4730826/76‑1 (MQ=255) cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAc > 1:81219/1‑76 (MQ=255) cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAc < 2:2884903/76‑1 (MQ=255) cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAc > 1:1642573/1‑76 (MQ=255) aTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa < 2:1685713/76‑1 (MQ=255) aTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa > 2:2301412/1‑76 (MQ=255) aTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAGAACa > 2:597281/1‑76 (MQ=255) tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAt < 1:4397008/76‑1 (MQ=255) tGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAAAt > 1:4801940/1‑76 (MQ=255) aTGTGCAGGCAGCGCAGCTGCGCTCGAAAATGCGCGGCATTGGTGTTAGCGTCACCCGCAGCGGGGATAACATTAt < 1:315053/76‑1 (MQ=255) tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATc < 1:4037567/76‑1 (MQ=255) gtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc < 2:2416090/76‑1 (MQ=255) gtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc > 1:1031914/1‑76 (MQ=255) tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCt < 1:63467/76‑1 (MQ=255) gCAGGAAGCGAAGCTGCGCGCCAAAATGCGCGGCACTGGTGTTAGCGTAGCCCGCAGCGGGGATAACATTATGCTc < 2:374014/76‑1 (MQ=255) gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc < 1:2811949/76‑1 (MQ=255) gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGAACCCCCCAGCGGGGAAACAATTACCCTc > 2:3437443/1‑76 (MQ=255) cAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCa > 1:3584960/1‑76 (MQ=255) aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCCGCGGGGATAACATTATCCTCaa < 1:665093/76‑1 (MQ=255) aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa > 2:2951490/1‑76 (MQ=255) aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa > 1:314295/1‑76 (MQ=255) aGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGCGTTAGCGTAACCCGCAGCGGGGACAACATTATCCTCaa > 1:4206760/1‑76 (MQ=255) ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat > 2:2894780/1‑76 (MQ=255) ggAAGCGAAGATGCGCGACAAAAAGCGCGGCACTGGTGATAGCGTAACCCGCAGCAGCGATAACATTATCGTcgat > 1:3466382/1‑73 (MQ=255) gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata < 1:584739/76‑1 (MQ=255) gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata > 1:884985/1‑76 (MQ=255) aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat < 1:3265884/76‑1 (MQ=255) aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat < 1:3613413/76‑1 (MQ=255) aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat < 2:1862621/76‑1 (MQ=255) || GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT > NC_000913/3714364‑3714504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |