| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,737,956 | 2 bp→CG | coding (623‑624/1254 nt) | avtA → | alanine‑alpha‑ketoisovalerate transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,737,956 | 0 | G | C | 100.0% | 286.0 / NA | 108 | G208A (GGC→GCG) | avtA | alanine‑alpha‑ketoisovalerate transaminase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (57/51); total (57/51) | |||||||||||
| * | NC_000913 | 3,737,957 | 0 | C | G | 99.1% | 285.9 / NA | 108 | G208A (GGC→GCG) | avtA | alanine‑alpha‑ketoisovalerate transaminase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (56/51); total (57/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG > NC_000913/3737888‑3738025 || ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGACGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa > 2:3563621/1‑76 (MQ=255)ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa > 1:425040/1‑76 (MQ=255)ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa > 1:4172929/1‑76 (MQ=255) tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac > 2:4266951/1‑76 (MQ=255) tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:3407938/76‑1 (MQ=255) tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:3404354/76‑1 (MQ=255) tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:2706793/76‑1 (MQ=255) tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:2696997/76‑1 (MQ=255) cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca > 2:4414528/1‑76 (MQ=255) ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac < 1:4533403/76‑1 (MQ=255) ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac > 1:4817555/1‑76 (MQ=255) ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac < 1:1907016/76‑1 (MQ=255) ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac < 2:4050565/76‑1 (MQ=255) cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg > 2:2155064/1‑76 (MQ=255) cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg < 1:3980403/76‑1 (MQ=255) gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc < 2:4812497/76‑1 (MQ=255) gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc < 1:4440374/76‑1 (MQ=255) gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc < 2:3574822/76‑1 (MQ=255) ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa < 1:3620751/76‑1 (MQ=255) cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTTCTGAAGCTTGACGCCCCTGCCAACCCACACCGCAt > 2:2388758/1‑76 (MQ=255) cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGGTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt > 1:3340659/1‑76 (MQ=255) cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt > 2:3683928/1‑76 (MQ=255) gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt > 1:1180075/1‑76 (MQ=255) gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt > 1:349862/1‑76 (MQ=255) acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATTAACACGGCATTc > 2:563244/1‑76 (MQ=255) acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc < 2:4481266/76‑1 (MQ=255) acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc < 1:55066/76‑1 (MQ=255) cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc > 1:2410654/1‑76 (MQ=255) cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc > 1:2456827/1‑76 (MQ=255) gaATCCAACAGTGAATGTGATTACTGACGAAGAGTTGCTGCAGCTTGACGCGCTGGCGAATCCACACGGCATTCCg < 2:2251878/76‑1 (MQ=255) gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCg > 2:2832678/1‑76 (MQ=255) aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTTCGc < 1:3110223/76‑1 (MQ=255) aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGc > 2:3641723/1‑76 (MQ=255) aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCGTGACGCGCTGGCGTATCAACACGGCGTTTCGc > 1:4843719/1‑76 (MQ=255) aTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCt > 1:4400828/1‑76 (MQ=255) accAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg < 2:161956/75‑1 (MQ=255) cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt < 2:402074/76‑1 (MQ=255) aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg < 2:4752813/76‑1 (MQ=255) aaCAGGCAAGGAGATTACTGACGAAGGGTTGCTGAAGCTTGACGCGCTCGCGTATCAACACGGCAGTCCGCTgggg > 1:3662392/1‑74 (MQ=255) aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtga > 2:1733170/1‑76 (MQ=255) aGGCAATGTGATTTCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt < 2:1503094/76‑1 (MQ=255) aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt > 1:2727449/1‑76 (MQ=255) aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt < 2:474668/76‑1 (MQ=255) aGGCAAGGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt < 2:776630/76‑1 (MQ=255) gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga > 1:1852234/1‑76 (MQ=255) gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga < 2:4521778/76‑1 (MQ=255) cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat > 2:935741/1‑76 (MQ=255) cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat < 1:3852041/76‑1 (MQ=255) aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa > 1:3999752/1‑76 (MQ=255) aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa > 1:3174051/1‑76 (MQ=255) aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa > 1:217296/1‑76 (MQ=255) tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc < 1:4335032/76‑1 (MQ=255) tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc > 2:1317490/1‑76 (MQ=255) gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg > 1:2225284/1‑76 (MQ=255) tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc < 2:2803859/76‑1 (MQ=255) gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt > 2:3325662/1‑76 (MQ=255) gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt > 1:4480463/1‑76 (MQ=255) gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGCGATTGATAACGCt > 2:119782/1‑76 (MQ=255) aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt < 1:1518895/76‑1 (MQ=255) aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATCGATAACGCtt < 2:425040/76‑1 (MQ=255) ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa < 1:2261566/76‑1 (MQ=255) tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTag > 2:4666534/1‑75 (MQ=255) aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg > 2:1820091/1‑76 (MQ=255) aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg < 1:2652358/76‑1 (MQ=255) cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg < 2:1602392/76‑1 (MQ=255) gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCg > 2:4371347/1‑76 (MQ=255) gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCg > 1:62389/1‑76 (MQ=255) gcGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt < 2:845655/75‑1 (MQ=255) aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt > 2:1870765/1‑76 (MQ=255) aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt > 2:4795568/1‑76 (MQ=255) aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt > 2:1527495/1‑76 (MQ=255) cGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc < 1:789550/76‑1 (MQ=255) cGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc > 2:3758826/1‑76 (MQ=255) gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc < 1:4794512/76‑1 (MQ=255) gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc > 2:4150801/1‑76 (MQ=255) aaGAGTTGCTGAACCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGAGTATGCTCATGGCATccc > 1:315670/1‑76 (MQ=255) agagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg < 1:479416/76‑1 (MQ=255) gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt < 1:2388759/76‑1 (MQ=255) gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCCTcccct > 2:10497/1‑74 (MQ=255) agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt > 1:3276121/1‑76 (MQ=255) gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc < 1:4084754/76‑1 (MQ=255) gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc < 2:392764/76‑1 (MQ=255) gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc > 1:703734/1‑76 (MQ=255) ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc > 1:4702414/1‑76 (MQ=255) ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc > 1:3770764/1‑76 (MQ=255) tGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc < 1:3497194/76‑1 (MQ=255) gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg < 2:3055524/76‑1 (MQ=255) gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg < 2:1371149/76‑1 (MQ=255) gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg > 1:545900/1‑76 (MQ=255) cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg < 2:3229760/76‑1 (MQ=255) aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGCTCCCGGCTa > 2:673415/1‑76 (MQ=255) aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCCGGTGATTGATAACGCTTATGGCGTCCCGTTCCAGGGTat > 1:502411/1‑76 (MQ=255) gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatc < 1:2289120/76‑1 (MQ=255) cTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca > 2:342698/1‑76 (MQ=255) ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat > 1:3522415/1‑76 (MQ=255) gtGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat < 1:161575/75‑1 (MQ=255) gcgcgCTGGCGAATCAACCCGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCtt < 1:4349804/75‑1 (MQ=255) cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc > 2:28110/1‑76 (MQ=255) cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc < 1:1840832/76‑1 (MQ=255) gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa > 2:2677267/1‑76 (MQ=255) || CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG > NC_000913/3737888‑3738025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |