| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,969,103 | 2 bp→GC | coding (232‑233/1263 nt) | wecC → | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,969,103 | 0 | C | G | 100.0% | 324.1 / NA | 121 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (67/54); total (67/54) | |||||||||||
| * | NC_000913 | 3,969,104 | 0 | G | C | 99.1% | 288.4 / ‑6.2 | 113 | R78A (CGC→GCC) | wecC | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (59/53); minor base A (1/0); total (60/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG > NC_000913/3969034‑3969179 || gCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc > 2:4117272/1‑76 (MQ=255) ggAGTGTAGAAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct < 1:1374291/75‑1 (MQ=255) gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 2:3330756/1‑76 (MQ=255) gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:4041187/1‑76 (MQ=255) gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:1717027/1‑76 (MQ=255) gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg > 1:2663188/1‑76 (MQ=255) aGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa > 2:871010/1‑76 (MQ=255) gtgtAGTAAAAACTGCCCTAGAAGGCGGCTTTTTACGACCGAGCCCCACGCCCGTTCCAGCGGATGCCTGGCTGAt > 2:4191409/1‑76 (MQ=255) tgtAGTAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 1:4794521/76‑1 (MQ=255) ggtagaaAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 1:118836/75‑1 (MQ=255) ggtagaaAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt < 2:2781518/75‑1 (MQ=255) tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc > 2:3428624/1‑76 (MQ=255) tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc < 1:2271795/76‑1 (MQ=255) tagtaAAAACTGCCGTAGAAGGCGGTGTTTTAAGAGCGAGCACGACGACAGTTGAAGCGGATGCCTGGCTGATTGc > 1:1824461/1‑76 (MQ=255) agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 2:2892103/1‑76 (MQ=255) agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt > 1:2964209/1‑76 (MQ=255) gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg > 2:3769657/1‑76 (MQ=255) aaaaacCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt < 1:1427065/75‑1 (MQ=255) aaaacCTGCCCGAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa < 2:4722714/76‑1 (MQ=255) aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc < 1:1950392/76‑1 (MQ=255) aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 2:3413025/1‑76 (MQ=255) aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 1:305846/1‑76 (MQ=255) aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc > 1:1523853/1‑76 (MQ=255) accTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc < 2:3231444/74‑1 (MQ=255) aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc > 2:2194196/1‑76 (MQ=255) aCTGCCGTAGAAGGCGGTTTTTTACGAGCGCGCACGACGCCCGTTGACGCGGAAGCCTGGCTGATTGCTGTACCCa < 2:3902153/76‑1 (MQ=255) cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc < 1:2048447/76‑1 (MQ=255) cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc > 1:4614897/1‑76 (MQ=255) tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 2:3550335/1‑76 (MQ=255) tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:1153287/1‑76 (MQ=255) tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg < 1:3632134/76‑1 (MQ=255) tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg > 1:1442411/1‑76 (MQ=255) ccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc < 1:780992/76‑1 (MQ=255) cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg < 1:4371878/76‑1 (MQ=255) cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg < 2:3646916/76‑1 (MQ=255) cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg > 1:184288/1‑76 (MQ=255) cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg < 2:3572358/76‑1 (MQ=255) gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 2:2898197/1‑76 (MQ=255) gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 1:1025357/1‑76 (MQ=255) gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt > 1:1072648/1‑76 (MQ=255) tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt > 2:2643802/1‑76 (MQ=255) aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt > 1:3652187/1‑76 (MQ=255) aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt < 2:454777/76‑1 (MQ=255) gaGGCGGTGTTTTACGAGCGAGCGCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa < 2:3431232/75‑1 (MQ=255) aaGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa > 1:1998876/1‑76 (MQ=255) aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg < 1:4369826/76‑1 (MQ=255) aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGCTTTAg > 2:1745993/1‑76 (MQ=255) ggCGGTTTTTTATGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTTATTGCTGTACCCTCGCCGTTTAAgg < 2:3465652/76‑1 (MQ=255) gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg < 1:2751846/76‑1 (MQ=255) gCGGTTTTTGACGAGCGCGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg < 1:2900240/76‑1 (MQ=255) cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc < 1:1032977/76‑1 (MQ=255) cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc > 1:816785/1‑76 (MQ=255) cGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTCTAACGGc > 2:280256/1‑76 (MQ=255) ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg > 1:785236/1‑76 (MQ=255) ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa < 2:3334029/76‑1 (MQ=255) ttggagcggggAGGACGGCAGGTGGAGGGGATGCCTGGGTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa < 2:327599/73‑1 (MQ=255) aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg < 1:4126167/76‑1 (MQ=255) aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg > 2:3961549/1‑76 (MQ=255) gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc < 1:4438203/76‑1 (MQ=255) gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc > 1:4193391/1‑76 (MQ=255) gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc < 2:816785/76‑1 (MQ=255) gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc > 1:2352170/1‑76 (MQ=255) gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGCTTAAGGGCGATCATGAGcc > 2:897350/1‑76 (MQ=255) agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa < 1:4120590/76‑1 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 2:2409803/1‑76 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:4238743/1‑76 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 2:840154/1‑76 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat < 2:3593269/76‑1 (MQ=255) gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat > 1:4640468/1‑76 (MQ=255) gagcACGACGCCAGCTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGAGAGATCATGAGCCAGaa > 2:755584/1‑75 (MQ=255) agcACGCCGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAtata < 1:617633/76‑1 (MQ=255) agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata < 1:78173/76‑1 (MQ=255) agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata < 2:4087419/76‑1 (MQ=255) agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata > 2:2384231/1‑76 (MQ=255) agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata > 2:2615513/1‑76 (MQ=255) cACGACGCGAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg < 2:3552266/76‑1 (MQ=255) cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg > 2:3253029/1‑76 (MQ=255) acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa < 2:1153286/76‑1 (MQ=255) cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc < 2:3411941/76‑1 (MQ=255) cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc < 2:3877891/76‑1 (MQ=255) gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc > 1:3255053/1‑76 (MQ=255) gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc > 2:4347594/1‑76 (MQ=255) gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc > 2:3814893/1‑76 (MQ=255) acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt < 1:2283747/76‑1 (MQ=255) acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt > 1:2576051/1‑76 (MQ=255) cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa < 1:3865297/76‑1 (MQ=255) gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATGATGAGCCAGATATGACCTAc < 2:1705841/76‑1 (MQ=255) gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc < 1:731339/76‑1 (MQ=255) cctgTTGAAGAGGATGCCGGGCTGATTGCTGGACCCACGCCGCTTAAGGGCGATCATCAGCCAGATATCACCTACg > 2:1320615/4‑76 (MQ=255) cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt < 1:3609951/76‑1 (MQ=255) aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt > 2:3512407/1‑76 (MQ=255) gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg > 2:3487649/1‑76 (MQ=255) tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa > 2:3692873/1‑76 (MQ=255) gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt < 2:4376377/76‑1 (MQ=255) gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt < 2:4238743/76‑1 (MQ=255) gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt > 1:4392666/1‑76 (MQ=255) aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 1:3167812/1‑76 (MQ=255) aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc > 2:1772286/1‑76 (MQ=255) aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg < 1:1781850/76‑1 (MQ=255) gctgggtgATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg < 1:328693/76‑1 (MQ=255) || GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG > NC_000913/3969034‑3969179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |