| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,971,075 | 2 bp→GC | coding (942‑943/1068 nt) | rffG → | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,971,075 | 0 | C | G | 99.1% | 277.0 / NA | 106 | C314W (TGC→TGG) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (55/50); minor base T (0/1); total (55/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| * | NC_000913 | 3,971,076 | 0 | G | C | 99.1% | 274.8 / ‑5.7 | 106 | V315L (GTG→CTG) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (54/51); minor base A (1/0); total (55/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTG > NC_000913/3971006‑3971143 || cGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGccc > 2:845508/1‑75 (MQ=255) gcggACGGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc < 2:818574/73‑1 (MQ=255) gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc < 1:2044636/76‑1 (MQ=255) gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc > 1:163485/1‑76 (MQ=255) gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc < 2:3194311/76‑1 (MQ=255) gCTGAACGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc > 1:3549968/1‑76 (MQ=255) cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCa > 1:2750742/1‑76 (MQ=255) cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCa < 1:2831175/76‑1 (MQ=255) cTGACCGTCCGGGGCATGATCTGCGTTATGCCATGGATGCTGCGAAAATTGCCCGGGAACTTGGCTGGCTGCCGCa < 1:2853846/76‑1 (MQ=255) tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg > 2:64286/1‑76 (MQ=255) tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg < 1:4471703/76‑1 (MQ=255) gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg < 1:824520/76‑1 (MQ=255) gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg < 2:1806424/76‑1 (MQ=255) aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa > 1:1080384/1‑76 (MQ=255) aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa > 2:1980595/1‑76 (MQ=255) ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaa < 1:3233504/76‑1 (MQ=255) ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaa > 1:2700878/1‑76 (MQ=255) ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaa > 2:504484/1‑76 (MQ=255) cggccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa < 2:2090593/73‑1 (MQ=255) cggccGGGGCATGATCTGCGTTATGCCATTGATGCGTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa < 2:2128338/73‑1 (MQ=255) cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa < 1:848795/76‑1 (MQ=255) cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa < 2:4841202/76‑1 (MQ=255) gTCGGGGGCATGGTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc < 2:188858/76‑1 (MQ=255) gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGACAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc > 1:651985/1‑76 (MQ=255) gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGacac > 2:2004246/1‑76 (MQ=255) gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc < 2:4631475/76‑1 (MQ=255) gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc < 2:2624431/76‑1 (MQ=255) gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc < 1:4384931/76‑1 (MQ=255) tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAct > 1:320147/1‑75 (MQ=255) tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAcc < 1:4334224/76‑1 (MQ=255) tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAcc > 2:4702408/1‑76 (MQ=255) ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt > 2:4572269/1‑76 (MQ=255) cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt < 1:3064931/76‑1 (MQ=255) ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCttn < 1:2635713/76‑2 (MQ=255) gggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTg < 2:3235722/76‑1 (MQ=255) gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa > 1:3519222/1‑76 (MQ=255) gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaa > 2:2485993/1‑76 (MQ=255) cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGCAACCTGTGcaa > 2:1973581/1‑76 (MQ=255) cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGaaa > 1:4075065/1‑76 (MQ=255) aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAACCCTTTGaaat < 2:3549968/76‑2 (MQ=255) aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAg > 1:117764/1‑76 (MQ=255) aTGATCTGCGTTATGCCATTGATGCGTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAg > 1:2342194/1‑76 (MQ=255) aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTgg < 2:2632752/76‑1 (MQ=255) tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa < 1:4407094/76‑1 (MQ=255) tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa > 2:2554473/1‑76 (MQ=255) cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa < 1:664544/76‑1 (MQ=255) tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt < 1:4550050/76‑1 (MQ=255) tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt < 2:1240555/76‑1 (MQ=255) gCGTTATGCCTTTGATGCTTCGAAAATTGCCCGTGAACTGGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATg < 2:2223676/76‑1 (MQ=255) gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATg < 2:2224052/76‑1 (MQ=255) gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATg < 2:2184709/76‑1 (MQ=255) gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATg > 2:1914283/1‑76 (MQ=255) gTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCg > 1:2013495/1‑76 (MQ=255) aTGCCATTGATGCTTCGCAACTTGCCCGTGACCTTGGCTGTCTGCCGCAGGACAGCCTTGAAAGTGGAATGCGTaa < 1:2784862/76‑1 (MQ=255) aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa > 1:4691735/1‑76 (MQ=255) aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa > 1:68761/1‑76 (MQ=255) tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGGATGCGGaaa > 2:188898/1‑76 (MQ=255) tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaaa < 2:1784628/76‑1 (MQ=255) ccATTGATGCTTCGACAATTGCCCGTGAACTTGGCTGGCTGCCGCCGCAAACCTTTGAACGCGGCACGCGTCAAAc > 2:193996/1‑76 (MQ=255) ccATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc > 2:2969165/1‑76 (MQ=255) cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACg < 1:1980596/76‑1 (MQ=255) ttGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt < 2:731837/76‑1 (MQ=255) aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa > 2:2772436/1‑76 (MQ=255) tGCTTCGAAGATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg > 1:3521727/1‑76 (MQ=255) tGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg < 2:2943281/76‑1 (MQ=255) cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTg > 2:4599229/1‑76 (MQ=255) ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTgg < 2:959620/76‑1 (MQ=255) ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTgg > 1:3330902/1‑76 (MQ=255) tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGGATGCGTAAAACGGTTCAGTGGt > 2:400905/1‑76 (MQ=255) tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGt < 2:4433542/76‑1 (MQ=255) tCGAAAATTGCCCGTGAACTCGGCTGGCTGCCGCAGGAAACCTTTGAAAGCGGAATGAGTAAACCGCATCAGCggg > 2:384609/1‑75 (MQ=255) cGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTa > 1:4073696/1‑76 (MQ=255) cGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTCGAAAGTGGAATGCGTAAAACGGTTCAGTGGTa < 2:2971771/76‑1 (MQ=255) gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAt > 1:1666168/1‑76 (MQ=255) aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc < 1:2282378/76‑1 (MQ=255) aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt > 1:1490264/1‑76 (MQ=255) aaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg > 2:1295874/1‑76 (MQ=255) aaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg > 2:3283531/1‑76 (MQ=255) ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGc > 1:1919971/1‑76 (MQ=255) tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt < 1:2681624/76‑1 (MQ=255) gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa < 1:3329828/76‑1 (MQ=255) cccGTGAGCTCGGCTGGCTGCCGCATGAAACCTTTGAAAGTGGAATTCGTAAAACGGTTCAGTGGTATCTGGCTaa < 1:2394753/76‑1 (MQ=255) cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGGTCAGTGGTATCTGGCTaa > 1:2029826/1‑76 (MQ=255) ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAt < 1:616165/76‑1 (MQ=255) ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAt > 1:90358/1‑76 (MQ=255) ccGTGAACTTGGCTGGCGGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAt < 1:632234/76‑1 (MQ=255) cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATg > 2:3217845/1‑76 (MQ=255) cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATg > 2:3328381/1‑76 (MQ=255) cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATATGGCTAATg > 2:2281268/1‑76 (MQ=255) gTGAACTTGGCTGGCTGCCGCAGGACACCTTTGAAAGTGGACAGCGTAAAACGGTTCAGTGGGATCTGGCTAATGa > 1:215708/1‑76 (MQ=255) gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaaa > 1:441780/1‑76 (MQ=255) gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaaa < 1:1532086/76‑1 (MQ=255) gcTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc < 2:3435485/75‑1 (MQ=255) aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAACCGGTTCAGTGGTATCTGGCTAATGAAAGc < 2:4648198/76‑1 (MQ=255) aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc > 2:4106014/1‑76 (MQ=255) cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCt > 2:3111395/1‑76 (MQ=255) cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCt < 2:2421232/76‑1 (MQ=255) cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCt > 1:1212485/1‑76 (MQ=255) ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg < 1:2216380/76‑1 (MQ=255) ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg < 2:163485/76‑1 (MQ=255) || CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTG > NC_000913/3971006‑3971143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |