| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,972,637 | 2 bp→GC | coding (430‑431/546 nt) | wecD → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,972,637 | 0 | C | G | 97.9% | 243.1 / ‑4.4 | 98 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (45/50); total (46/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| * | NC_000913 | 3,972,638 | 0 | G | C | 99.0% | 248.4 / NA | 99 | R144A (CGT→GCT) | wecD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (45/52); minor base T (0/1); total (46/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCG‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT > NC_000913/3972569‑3972704 || gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCgg > 1:3562088/1‑76 (MQ=255)gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCgg > 1:2466837/1‑76 (MQ=255)gAATGGGCGTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCgg < 2:4720266/76‑1 (MQ=255) aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCggg > 2:1503881/1‑75 (MQ=255) ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCATATGC‑TCGCGGTaa < 2:894182/76‑1 (MQ=255) tGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTaaa < 2:1976293/76‑1 (MQ=255) ggCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTaaaa < 2:3119777/76‑1 (MQ=255) gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTCTGC‑CCGCGGTcaaaa > 2:163098/1‑75 (MQ=255) gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGGaaacc > 2:4716749/1‑74 (MQ=255) ccggctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca < 2:567459/73‑1 (MQ=255) ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca > 2:1629674/1‑76 (MQ=255) ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca < 2:3591133/76‑1 (MQ=255) ccTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaaca < 1:2538474/76‑1 (MQ=255) tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAaacaac > 1:129359/1‑76 (MQ=255) gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACt < 1:2142482/76‑1 (MQ=255) gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAAATGGGCGTATGC‑TCGCGGGAaaaaaaat > 1:2929825/1‑74 (MQ=255) ctggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACtt < 2:2632803/76‑1 (MQ=255) tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACttt > 2:4411707/1‑76 (MQ=255) ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:1948162/76‑1 (MQ=255) ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 1:2800779/76‑1 (MQ=255) ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:2641966/76‑1 (MQ=255) ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:347997/76‑1 (MQ=255) ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTg < 2:3506188/76‑1 (MQ=255) gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGc < 2:677256/76‑1 (MQ=255) gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGc < 1:2277429/76‑1 (MQ=255) gtggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCg < 2:4084688/75‑1 (MQ=255) tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCgg > 2:3342727/1‑76 (MQ=255) tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCgg > 1:3863630/1‑76 (MQ=255) tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑CCGCGGTAAAACAACTTTGccg > 2:207447/1‑74 (MQ=255) ggACGCGGTGCGTGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCggg < 2:814451/76‑1 (MQ=255) ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCggg > 1:2820920/1‑76 (MQ=255) gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGTGGGTg < 2:4793592/75‑1 (MQ=255) gcgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTg < 2:4120306/75‑1 (MQ=255) gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGc > 2:3291629/1‑76 (MQ=255) gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGc < 1:1640184/76‑1 (MQ=255) gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGc > 1:4829185/1‑76 (MQ=255) cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCg < 1:4076754/76‑1 (MQ=255) cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCg < 1:2353610/76‑1 (MQ=255) cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGGGGCg > 2:1897794/1‑76 (MQ=255) cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑GCGCGGTAAAACAACTTTGCGGGTGGCg < 1:4374971/76‑1 (MQ=255) gTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAc < 1:271802/76‑1 (MQ=255) tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAcc > 2:2689546/1‑76 (MQ=255) ggCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAcc < 2:4523851/75‑1 (MQ=255) gCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGAccc > 1:986302/1‑76 (MQ=255) cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCa > 1:2556182/1‑76 (MQ=255) cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGCGGCCACCCa > 2:1160509/1‑76 (MQ=255) aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa < 1:4219208/76‑1 (MQ=255) aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa > 1:1429084/1‑76 (MQ=255) ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACCACTTTGCGGGTGGCGCCCCaaa > 2:4820345/1‑76 (MQ=255) ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa < 1:959168/76‑1 (MQ=255) ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa < 1:2060543/76‑1 (MQ=255) ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa > 1:4597180/1‑76 (MQ=255) gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt < 1:164117/76‑1 (MQ=255) gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt < 2:2359025/76‑1 (MQ=255) gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg > 2:2002972/1‑76 (MQ=255) gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg < 2:2679605/76‑1 (MQ=255) gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg > 2:1773589/1‑76 (MQ=255) ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg > 1:2748999/1‑76 (MQ=255) ctgagctgaTGCAAACGGCGCTAAACTGGGCGTA‑CGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg > 1:4130212/1‑76 (MQ=255) gagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa < 2:1826240/76‑1 (MQ=255) gagctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa > 1:3848755/1‑76 (MQ=255) gggtgaTGCAAGCGGCGGTAGTCTGGGCGTATGT‑TCGCGGTAAAACAACTTTGCTGGTGGCGACCCAAATGGGCaa < 2:4723187/73‑1 (MQ=255) ggctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa < 2:1180791/75‑1 (MQ=255) agctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa > 1:1277817/1‑76 (MQ=255) ctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca < 2:3397853/76‑1 (MQ=255) ctgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca < 2:3975595/76‑1 (MQ=255) tgaTGCAAGCGGCGCTCAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGCCCCAAATGGGCAacac < 1:401259/76‑1 (MQ=255) tgaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac < 1:2858290/76‑1 (MQ=255) gaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc > 2:2653047/1‑76 (MQ=255) gaTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAGGGGCAACAcc > 2:1642068/1‑76 (MQ=255) aTGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg > 2:3928940/1‑76 (MQ=255) tGCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGGGGCGCCCCAAATGGGCCACAcccc > 2:19140/1‑74 (MQ=255) gCAATCGGCGCGAAACTGGGCGTAGTC‑TCGCGGTAAAGCAACTTTGCGGGTGGCGACCCAAATGGGCACCAccgcc < 1:3121189/76‑1 (MQ=255) gCAAACGGCGCTAACCGGGGCGTATGC‑TCGCGGTAAAACACCTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:2429788/76‑1 (MQ=255) gCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 1:4105566/76‑1 (MQ=255) gCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:4278773/76‑1 (MQ=255) gCAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 1:3763278/76‑1 (MQ=255) gCAAACGGCGCTAAACGGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc < 2:436736/76‑1 (MQ=255) cAAACGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgccg > 1:2556576/1‑76 (MQ=255) aagcGGCGCTAACCGGGGCGTATGC‑TCGCGGTAAAACATCTTTGGGGGTGGCGACCAAAAGGGGCAACACcgccgc < 1:4200378/73‑1 (MQ=255) aaccGGCGCTAAACGGGGGGTATGC‑TCGCGTAAAACCAACTTGGCGGGTGGCGACCAAAAGGGCCACCCCcgccgc < 1:4106704/73‑1 (MQ=255) aaaCGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACcgccgc > 1:4147046/1‑76 (MQ=255) aCGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc < 1:1962912/76‑1 (MQ=255) cGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt > 2:3805876/1‑76 (MQ=255) cGGCGCTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt > 2:2883859/1‑76 (MQ=255) cGGCGCGAAACTGGGCGTATGC‑TCGCGGTAAAACATCTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt < 2:4293248/76‑1 (MQ=255) ggCGCTAAACGGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt < 1:2203413/76‑1 (MQ=255) cgcTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa > 1:185870/1‑76 (MQ=255) gcTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa > 2:3753872/1‑76 (MQ=255) gcTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa > 2:4387586/1‑76 (MQ=255) cTAAGCGGGGCGTATGC‑GCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc < 2:1310995/76‑1 (MQ=255) cTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 1:2945781/1‑76 (MQ=255) cTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 2:3236861/1‑76 (MQ=255) cTAAACTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc > 2:3021310/1‑76 (MQ=255) aCTGGGCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata > 1:751037/1‑76 (MQ=255) aCTGGGCGTATCC‑TCGGGGAAAAACACCTGTGCGGGTGGCGCCCCAAAGGGGCAACACCGCCGCGCTTAAACGata < 1:1104903/76‑1 (MQ=255) cggggCGTATCC‑CGGGGTTAAACCACCTTGGCGGGTGGGGCCCCAAGGGGGCAACCCCGCCGCGCTTAAACGatac < 2:3805019/74‑1 (MQ=255) gggCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacat > 1:2613564/1‑76 (MQ=255) gggCGTATGC‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacat > 2:4275506/1‑76 (MQ=255) || GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCG‑TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT > NC_000913/3972569‑3972704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |