| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,918 | 2 bp→CG | coding (348‑349/1236 nt) | aslB → | putative arylsulfatase regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,918 | 0 | G | C | 98.6% | 380.2 / NA | 148 | W116C (TGG→TGC) | aslB | putative arylsulfatase regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base C (65/80); total (66/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,980,919 | 0 | C | G | 98.7% | 392.5 / NA | 150 | P117A (CCG→GCG) | aslB | putative arylsulfatase regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (66/82); minor base T (0/2); total (66/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAAT > NC_000913/3980849‑3980988 || atatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt > 2:1783343/1‑76 (MQ=255)atatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt > 2:4490554/1‑76 (MQ=255) tatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtc < 2:4024256/76‑1 (MQ=255) atGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct < 1:983203/76‑1 (MQ=255) tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc < 1:3544620/76‑1 (MQ=255) tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc < 2:3548289/76‑1 (MQ=255) tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc < 2:35584/76‑1 (MQ=255) ggTGCTGGCAGGAAGATAAGTAAGAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcg < 2:1067416/76‑1 (MQ=255) gTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc < 2:1733622/76‑1 (MQ=255) tGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg < 2:3631192/76‑1 (MQ=255) gggtGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg < 2:1078746/73‑1 (MQ=255) gCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCgg > 2:4412672/1‑76 (MQ=255) gCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCgg > 2:4480327/1‑76 (MQ=255) cTGGCAGGAATATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa < 1:4859129/76‑1 (MQ=255) cTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa < 1:3180563/76‑1 (MQ=255) tGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAg > 2:1962807/1‑76 (MQ=255) tGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAg < 1:1160025/76‑1 (MQ=255) ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc < 1:4100248/76‑1 (MQ=255) ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc < 2:1714846/76‑1 (MQ=255) gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca < 2:3486034/76‑1 (MQ=255) gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca < 1:4213237/76‑1 (MQ=255) aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatc < 2:1318877/76‑1 (MQ=255) aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatc < 1:54201/76‑1 (MQ=255) aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatc > 2:3863605/1‑76 (MQ=255) aaGATAAGTAGCGGCTTCCAGACTAACGGCGTGTGACTGGATGACGAATTGTGCGCGTTTCTCGCGGAGCAGCAtt < 1:4296610/76‑1 (MQ=255) aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAttt < 2:263174/76‑1 (MQ=255) gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCCTCAtttt > 2:2006660/1‑76 (MQ=255) gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt < 2:1743973/76‑1 (MQ=255) gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt > 1:3103520/1‑76 (MQ=255) aTAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTc > 2:494686/1‑76 (MQ=255) aTAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTc > 1:3655132/1‑76 (MQ=255) aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCtt < 2:792308/76‑1 (MQ=255) tAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt > 1:79661/1‑76 (MQ=255) tAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt > 1:3463004/1‑76 (MQ=255) tAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt > 1:4311876/1‑76 (MQ=255) aaCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGGCCGCGTTTCCCGCGGACCACCATTCTCttgttg > 2:310575/1‑76 (MQ=255) aCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTgg > 1:3995354/1‑76 (MQ=255) aCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTgg > 1:2478962/1‑76 (MQ=255) cAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt < 1:3804077/76‑1 (MQ=255) cAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt > 2:2529262/1‑76 (MQ=255) accTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGCGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt > 2:2015321/3‑76 (MQ=255) aGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTggat > 1:2158277/1‑74 (MQ=255) aGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt > 2:2142091/1‑76 (MQ=255) aGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt > 1:4540393/1‑76 (MQ=255) ctgccAGACTAACGGCGGGGTGCTGGATGACGAATGGTGCGCGTGTCGCGCGGAGCATCATTGTCTTGTTGGTTTa < 2:2864534/73‑1 (MQ=255) cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa < 2:3422861/76‑1 (MQ=255) tCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATc < 1:4579970/76‑1 (MQ=255) tCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATc < 1:1955210/76‑1 (MQ=255) tCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATc > 1:2640718/1‑76 (MQ=255) ctaGACGAGCGGCGTTCTGCTGGATTACGAATGGTGCGCGTTTCTCGCGGATCATCATTTTCTTGTTGGTTTATCg < 2:3281223/74‑1 (MQ=255) ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg < 1:1356690/76‑1 (MQ=255) ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg > 2:3841881/1‑76 (MQ=255) ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg > 1:3635357/1‑76 (MQ=255) cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc < 1:4151793/76‑1 (MQ=255) cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc < 2:2026088/76‑1 (MQ=255) aGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt > 1:2915843/1‑76 (MQ=255) aGACTAACGGCGGGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt < 2:2244585/76‑1 (MQ=255) gACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTg < 2:3123815/76‑1 (MQ=255) gACTAACGGCGTGCTGCTGGATGACGAAGGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTg < 1:3101404/76‑1 (MQ=255) aCTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCggg > 1:2431159/1‑76 (MQ=255) aCTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg < 1:83744/76‑1 (MQ=255) tAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAt > 2:2772848/1‑76 (MQ=255) aaCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg > 2:421122/1‑76 (MQ=255) ggCGTGCTGCTGGATGCCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTGTGTTTATCGCTGGATggac > 1:3392518/1‑74 (MQ=255) ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc > 1:4489493/1‑76 (MQ=255) ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc > 1:4002955/1‑76 (MQ=255) ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc > 1:3355790/1‑76 (MQ=255) ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc < 1:1572011/76‑1 (MQ=255) cgggTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc < 2:3865615/73‑1 (MQ=255) gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGccc < 2:2252186/76‑1 (MQ=255) gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGccc > 1:3027784/1‑76 (MQ=255) gttgctgcTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCg < 1:3192017/74‑1 (MQ=255) gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGc > 2:3374319/1‑76 (MQ=255) gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGc > 1:2620337/1‑76 (MQ=255) tgctgcTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc < 1:1565268/76‑1 (MQ=255) tgctgcTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc > 1:1150888/1‑76 (MQ=255) gctgctGGATGACGAATGATGCTCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt < 1:4114615/76‑1 (MQ=255) ctgctgGATGTCTGATGGTTGGCTTTTCTCGCGGATGATCGTTGTCTTGTTGGTTTATCTCTGGATGGCCCGCCTg < 1:2174068/76‑1 (MQ=255) tgctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTga < 1:2406104/76‑1 (MQ=255) gctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgag < 1:1406876/76‑1 (MQ=255) gctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgag > 1:4695349/1‑76 (MQ=255) gtgGTTGAGGAAGGGTGCGCGTTTGTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgaga < 2:4749695/75‑1 (MQ=255) ctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgaga < 1:3863605/76‑1 (MQ=255) tgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAt > 2:3187217/1‑76 (MQ=255) tgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAt < 2:3060640/76‑1 (MQ=255) ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATc > 2:1631907/1‑76 (MQ=255) gaggACGAAGGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc < 2:3400667/73‑1 (MQ=255) gATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc < 1:2478991/76‑1 (MQ=255) gATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc > 2:2902029/1‑76 (MQ=255) aTGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCca > 1:1709207/1‑76 (MQ=255) tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac > 1:1864308/1‑76 (MQ=255) tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac > 1:1622680/1‑76 (MQ=255) tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac > 1:3768188/1‑76 (MQ=255) gACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGTCGGATGGCCCGCCTGAGTTCcaca > 2:4137611/1‑76 (MQ=255) gACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcaca > 2:3418200/1‑76 (MQ=255) gACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcaca > 2:2735377/1‑76 (MQ=255) gAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc < 2:1880216/76‑1 (MQ=255) gAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTCGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc > 1:4577957/1‑76 (MQ=255) aaTGGTGCTCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatca < 2:465433/76‑1 (MQ=255) aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatca > 1:3909163/1‑76 (MQ=255) || ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAAT > NC_000913/3980849‑3980988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |