| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,001,421 | 2 bp→GC | coding (381‑382/903 nt) | rarD ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,001,421 | 0 | C | G | 98.8% | 218.0 / NA | 84 | G128R (GGA→CGA) | rarD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (43/40); minor base T (0/1); total (43/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,001,422 | 0 | G | C | 100.0% | 223.9 / NA | 84 | D127E (GAC→GAG) | rarD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (43/41); total (43/41) | |||||||||||
AAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGT > NC_000913/4001352‑4001488 || aaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGcc > 1:4000656/1‑76 (MQ=255) agcccACAGCTGGACTAACCCCCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCg < 1:235918/73‑1 (MQ=255) aGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCg > 1:264810/1‑76 (MQ=255) tCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAg > 2:2218786/1‑76 (MQ=255) tCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAg > 2:1510768/1‑76 (MQ=255) cccACGGCGGGACTAACCCGCCCCATATCGCCAGAACCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAg < 2:3204900/75‑1 (MQ=255) cacaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGa > 2:2740782/1‑76 (MQ=255) cacaGCTGGACTAACACGCCACATATCGCCAGAATAACCGCCAGCCATTGCATCCGGCGCAATCGCTCGCCGacga > 1:2605635/1‑73 (MQ=255) acaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAggca > 2:2174346/1‑74 (MQ=255) acaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaa > 1:2001807/1‑76 (MQ=255) caccTGAACAAACACCCCACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaa < 1:4789629/73‑1 (MQ=255) aGCTGGACTAGCCCGCCACATATCGCGAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaaa < 1:3870085/76‑1 (MQ=255) aGCTGGACTAACACGCCACATAGCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaaa < 2:1442809/76‑1 (MQ=255) gCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAat > 2:4559916/1‑76 (MQ=255) ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGCGGAAAatcac > 2:65033/1‑75 (MQ=255) ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGCGGAAAatcac > 2:411702/1‑75 (MQ=255) gACTAACACCCCACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAatcatc < 2:4111181/76‑1 (MQ=255) acaaaCACCCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcc < 1:3986662/73‑1 (MQ=255) cTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAAACATccc > 2:3738697/1‑76 (MQ=255) tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcccc > 2:2863194/1‑76 (MQ=255) aaCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCa > 2:1588051/1‑76 (MQ=255) acacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCac > 2:1557091/1‑75 (MQ=255) acacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAg > 1:2671861/1‑76 (MQ=255) acacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAg > 2:1548266/1‑76 (MQ=255) acacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAg > 2:1322151/1‑76 (MQ=255) cacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGc > 2:248541/1‑76 (MQ=255) cGCCCCATACCGCCAGAACCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGcac < 2:36630/76‑1 (MQ=255) cGCCACATATCCCCAGAACCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGcac < 2:3267811/76‑1 (MQ=255) gCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAGATCATCCCCAGcaca > 1:3060966/1‑76 (MQ=255) gCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGcaca > 1:1563539/1‑76 (MQ=255) gCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGcaca > 1:1986874/1‑76 (MQ=255) ccACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACaa < 1:3718552/76‑1 (MQ=255) ccaTACCGCCAGAACCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATg < 1:2508509/75‑1 (MQ=255) caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGt > 1:3823837/1‑76 (MQ=255) agatCGCCAGAACCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGtt < 2:1402767/74‑1 (MQ=255) tgccGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc < 2:616181/73‑1 (MQ=255) tatCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc < 2:3866041/76‑1 (MQ=255) tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc > 2:3586835/1‑76 (MQ=255) tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc > 1:3668195/1‑76 (MQ=255) atCGCAAGAACCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa < 1:4161808/76‑1 (MQ=255) accGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa < 1:30771/74‑1 (MQ=255) tCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAc > 1:572798/1‑76 (MQ=255) cGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAcc > 2:4417705/1‑76 (MQ=255) gCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCa > 2:1301649/1‑76 (MQ=255) gCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCa > 1:3129208/1‑76 (MQ=255) cccAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCa < 1:3766119/75‑1 (MQ=255) ccAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAg < 2:3820757/76‑1 (MQ=255) cAGAATCAGCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGc < 2:4646451/76‑1 (MQ=255) aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCg > 2:3848825/1‑76 (MQ=255) aGAACCACCCCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCg < 2:120969/76‑1 (MQ=255) accACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGt < 1:1618685/74‑1 (MQ=255) cgccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta < 1:4216574/74‑1 (MQ=255) cACCGCCAGCCATCGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta > 2:1691765/1‑76 (MQ=255) tccGCGATCCATTGCATCCGGCGGACTGTCTCGCCGAGGAAAATCATGCGCAGCCGAATGTTCACGCGCGTGTtaa < 2:2098004/75‑1 (MQ=255) cccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaa < 1:713260/75‑1 (MQ=255) cccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaa < 1:25232/75‑1 (MQ=255) ccGCCAGCCATTGCATCCGGCGGAATCGCCCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTcaat > 2:858307/1‑76 (MQ=255) cGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaata < 1:1391535/76‑1 (MQ=255) cGCCAGCCATTCCATCCGGCGGAATCGCTAGCAGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGCtaata > 2:460176/1‑76 (MQ=255) gccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaataa > 1:2231124/1‑76 (MQ=255) ccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa < 2:595459/76‑1 (MQ=255) ccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa > 2:4357789/1‑76 (MQ=255) cagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAGATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg < 2:3060966/76‑1 (MQ=255) cagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg < 1:1548267/76‑1 (MQ=255) cagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg < 1:2901594/76‑1 (MQ=255) agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTACTAAAga > 2:4751650/1‑75 (MQ=255) agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGt < 1:4794418/76‑1 (MQ=255) ccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTaa < 2:3132842/76‑1 (MQ=255) caTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAACGTAAc > 2:1644569/1‑76 (MQ=255) aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAcc > 2:4536660/1‑76 (MQ=255) aTTGCATCCGGCGGAATCGCTAGCCGAGGAAAACCATCCCCAGCACAATGTTCAACAGCGCGCTAATAAAGTaaac > 1:2924400/1‑74 (MQ=255) tGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCaa > 1:2498578/1‑76 (MQ=255) gCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAg > 2:3922777/1‑76 (MQ=255) cATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAACAGCGGGTTAATAAAGTAACCAAgg < 1:3094827/76‑1 (MQ=255) tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCt < 2:1180805/76‑1 (MQ=255) ccGGCGGAATCGCTCGCCGATGAAAATCATGCCCAGCACAATGTTCACCGGCGGGTTAATATAGTAACCAAGGCTc < 1:460176/76‑1 (MQ=255) ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACGAAGGCTc < 2:1930327/76‑1 (MQ=255) ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc < 1:4048576/76‑1 (MQ=255) ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc < 2:1447906/76‑1 (MQ=255) ccGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc < 2:3129208/76‑1 (MQ=255) gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgct < 1:4300288/76‑4 (MQ=255) gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgct > 1:2423106/1‑73 (MQ=255) gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgct < 1:4447477/76‑4 (MQ=255) gcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGCTCACCAGCGGGTTAATAAAGTAACCAACGCTcgct > 1:1893848/1‑73 (MQ=255) || AAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGT > NC_000913/4001352‑4001488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |