| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,008,491 | 2 bp→CG | intergenic (+58/‑174) | b4555 → / → yigM | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,008,491 | 0 | G | C | 99.0% | 253.5 / ‑5.8 | 100 | intergenic (+58/‑175) | b4555/yigM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (40/59); total (40/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
| * | NC_000913 | 4,008,492 | 0 | C | G | 99.0% | 261.7 / NA | 100 | intergenic (+59/‑174) | b4555/yigM | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (39/60); minor base A (1/0); total (40/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTTGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCC > NC_000913/4008427‑4008559 || ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCcgcg > 2:3397167/4‑76 (MQ=255) tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCgc > 2:1147751/3‑75 (MQ=255) tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGt < 2:1434903/74‑1 (MQ=255) tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGt < 2:2873027/74‑1 (MQ=255) gtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt < 1:2379453/76‑1 (MQ=255) gACGGGATGAACGACGCCGAAATGCTGTCGACGGC‑GGCGAAAGGCTGCATTATAGGCAGCGCACACCAGCGTCtca > 1:3894669/1‑74 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTgca > 1:3810150/1‑74 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGaa > 2:3618591/1‑76 (MQ=255) aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGaa < 2:3435984/76‑1 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAg > 1:2512601/1‑76 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAg < 1:4318325/76‑1 (MQ=255) gggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGCGCGCACCACCGTCTGaaac > 2:898294/1‑75 (MQ=255) gggtGAACGACGCGGAAATGCTGTGGGGGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGa < 2:4739062/73‑1 (MQ=255) ggATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGa < 2:1680721/76‑1 (MQ=255) gATGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAc < 1:4349596/76‑1 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCCGTGCGCACCAGCGTCTGCAACAcc > 2:1443325/1‑76 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAcc > 2:4304880/1‑76 (MQ=255) aTGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAcc > 2:3123186/1‑76 (MQ=255) tGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCt < 2:3339859/76‑1 (MQ=255) tGAACGACGCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCt < 1:3361916/76‑1 (MQ=255) gAACGACGCCGAAATGCTGTCGATGACGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCtt < 1:2092071/76‑1 (MQ=255) tgcgacgCCGTGATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCATTTGGCGCCAGCTTCTGAAAGACCTTc < 2:3167314/74‑1 (MQ=255) gacgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc > 2:554602/1‑76 (MQ=255) gacgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGGGCGCACCAGCGTATGAAAGACCTTCCTc > 2:1316775/1‑76 (MQ=255) gacgCCGAAATGCGGGCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc < 2:3667113/76‑1 (MQ=255) acgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc > 2:2990825/1‑76 (MQ=255) acgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc < 2:3045707/76‑1 (MQ=255) cgtcGAAATGCGGCCGTTGGGTGG‑GAAATGCTGTCTTATGGGCCGTGCGCACCAGCGTCTGACCGACCTTCATccc < 2:1932311/73‑1 (MQ=255) cgCCGAGATGCTGGCGAGGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACGTTCATccc < 2:972160/76‑1 (MQ=255) cgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc > 2:2733047/1‑76 (MQ=255) cgCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc < 2:2481421/76‑1 (MQ=255) gCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCCGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg > 1:3054200/1‑76 (MQ=255) gCCGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg > 1:1076628/1‑76 (MQ=255) ccGAGATGCTGGCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa < 2:265884/76‑1 (MQ=255) ccGAATTGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGTCCTTCCTCCCGa < 1:1443252/76‑1 (MQ=255) ccGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa > 2:2528308/1‑76 (MQ=255) ccGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa < 1:3326156/76‑1 (MQ=255) cGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg > 1:4074132/1‑76 (MQ=255) cGAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg > 1:4465115/1‑76 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc > 1:1466152/1‑76 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc < 1:109247/76‑1 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc < 2:2818113/76‑1 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc < 2:3924921/76‑1 (MQ=255) gAAATGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGCGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc > 2:3773430/1‑76 (MQ=255) acaTGCTGTCGAGGGCGGG‑GAAAGGCTGCATTATGTGCAGTGCGCGCCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:2584880/74‑1 (MQ=255) aaaTGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt < 1:3760596/76‑1 (MQ=255) aTGCTGGCGATGGCGGG‑GAAAGGCTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg < 2:3448801/76‑2 (MQ=255) tGCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga > 1:730843/1‑74 (MQ=255) gCTGTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa < 1:2076657/76‑4 (MQ=255) gTCGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTg > 2:3016000/1‑76 (MQ=255) cGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt < 2:3835462/76‑1 (MQ=255) cGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt < 1:2434551/76‑1 (MQ=255) cGATGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt < 2:3796450/76‑1 (MQ=255) aTGGCGGG‑GAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTg > 1:3514535/1‑76 (MQ=255) gcggg‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTa > 1:1469020/4‑76 (MQ=255) gcggg‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTa < 2:538722/73‑1 (MQ=255) cggg‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTAc < 2:3054200/74‑1 (MQ=255) ggg‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACt > 1:594061/2‑76 (MQ=255) gg‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑CAGTGATTGGTACTa > 2:1580058/1‑76 (MQ=255) gg‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTa > 1:3900375/1‑76 (MQ=255) gg‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTa > 2:2664109/1‑76 (MQ=255) g‑gAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAGCTTCATCCCGAGCTGG‑AAGTGATTGGTACTaa < 2:4094724/76‑1 (MQ=255) gAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATg < 2:1146351/76‑1 (MQ=255) aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGc > 2:2895791/4‑76 (MQ=255) aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGc > 2:737636/4‑76 (MQ=255) aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGcc < 1:2780817/74‑1 (MQ=255) aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGcc < 1:4548023/74‑1 (MQ=255) ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg < 1:1211114/75‑1 (MQ=255) ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg > 1:1435341/2‑76 (MQ=255) ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg > 2:361590/2‑76 (MQ=255) gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcga < 1:673755/76‑1 (MQ=255) gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcga < 1:1967565/76‑1 (MQ=255) tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacg > 2:4000117/1‑76 (MQ=255) tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacg > 2:4152501/1‑76 (MQ=255) gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑GAGTGATTGGTACTAATGCcgacga > 2:1422163/1‑76 (MQ=255) gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacga < 2:615949/76‑1 (MQ=255) gCATTAGGGGCAGGGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacga < 2:150081/76‑1 (MQ=255) cATTATGGGCAGTGCGCGCCAGCGTCTGAAAGACCTGCATCCCGAGCT‑GTAAGTGATTTGTACTAATGCCgacgac < 1:3405968/76‑1 (MQ=255) cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGATGTGG‑AAGTGATTGGTACTAATGCCgacgac < 1:2559158/76‑1 (MQ=255) cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac < 1:2516127/76‑1 (MQ=255) cATTATGGGCAGTGCGCACCAGCGTCTGAAAGAACTTCATCCCGAGCTGG‑ACGGGATTGGTACTAATGCCgatgac > 2:3241215/1‑76 (MQ=255) cATTAGGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac < 2:3646296/76‑1 (MQ=255) aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGacgacg < 1:2410777/76‑1 (MQ=255) ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgc < 1:3542838/76‑1 (MQ=255) tgaTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgc < 1:511002/74‑1 (MQ=255) tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg < 1:2892318/76‑1 (MQ=255) tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg < 2:4465115/76‑1 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGCCCACGCgg > 2:4799969/1‑76 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCgg > 2:3589337/1‑76 (MQ=255) aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCgg > 2:3584729/1‑76 (MQ=255) tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt < 2:4258628/76‑1 (MQ=255) tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt < 1:4103645/76‑1 (MQ=255) tGGGCAGGGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt < 2:582257/76‑1 (MQ=255) gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTg < 1:2213306/76‑1 (MQ=255) gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTg < 2:2123945/76‑1 (MQ=255) gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTg < 1:4668228/76‑1 (MQ=255) gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTg < 2:2545943/76‑1 (MQ=255) ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGc < 1:898295/76‑1 (MQ=255) gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGcc < 2:3856558/76‑1 (MQ=255) gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGcc < 2:3238767/76‑1 (MQ=255) || GTTGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCC > NC_000913/4008427‑4008559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |