| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,026,252 | 2 bp→GC | coding (110‑111/1164 nt) | fadA ← | thiolase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,026,252 | 0 | C | G | 99.1% | 294.7 / ‑4.6 | 113 | T37T (ACG→ACC) | fadA | thiolase I |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (58/54); total (58/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
| * | NC_000913 | 4,026,253 | 0 | G | C | 99.1% | 294.8 / ‑3.6 | 115 | coding (110/1164 nt) | fadA | thiolase I |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (59/55); minor base . (0/1); total (59/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC > NC_000913/4026183‑4026320 || ctgctgCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGc > 1:3971911/1‑76 (MQ=255) ctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCAtt < 1:4154540/76‑1 (MQ=255) tgcACACACCCCCAGTAAATATCGGCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTa < 2:1384036/76‑1 (MQ=255) tgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCCGCA‑GGCTACGCCTTa > 2:3716955/1‑76 (MQ=255) tgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCC‑GGCTACGCATTa > 1:619341/1‑76 (MQ=255) gcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GCCTACGCAGTaa > 2:2668995/1‑76 (MQ=255) cacacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTaaa > 1:4422792/1‑76 (MQ=255) cacacaACCCCAGTAAATATCGGCGGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTaaa < 2:2265813/76‑1 (MQ=255) ccacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAAt < 2:3629749/75‑1 (MQ=255) acacaACCCCAGTAAATATCGTCGAGGGCCCCCGCTTCCCGCGCCGGGTTCCGCGCCCGCA‑GGCTCCGCATTAAAt > 2:4129988/1‑76 (MQ=255) cacaCCCCCAGTAAATAGCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATg < 1:1168438/76‑1 (MQ=255) cacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATg < 2:2008693/76‑1 (MQ=255) aacaACCCCAGTAAGTAGGTGGGAGGGCCGGCGCTTCCAGCGACGGGTTACGCGCCAGCA‑GGCTACGCATTAAATg < 2:1491667/75‑1 (MQ=255) acaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGa < 2:3971911/76‑1 (MQ=255) cacccccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAg < 2:2417930/73‑1 (MQ=255) caACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAg > 1:1355167/1‑76 (MQ=255) cccccAGAAAATATCGTCGAGGCCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCg < 1:2704799/75‑1 (MQ=255) aCCCCAGTAAATATCGTCGGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCg < 2:2621629/76‑1 (MQ=255) aCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCg > 1:4647366/1‑76 (MQ=255) aCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCg > 1:4353709/1‑76 (MQ=255) aCCCCAGAAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCg < 1:1592444/76‑1 (MQ=255) ccccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCgg < 2:2771831/76‑1 (MQ=255) ccAGAAAATACCGTCGGGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGgag < 2:4632381/76‑1 (MQ=255) gTAAATATCGTTGAGGGCCGCCGGTTGCAGCGCCGGGGTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGtagaga > 1:542163/1‑76 (MQ=255) gTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGgagaga < 1:4113809/76‑1 (MQ=255) tAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGAt > 1:948676/1‑76 (MQ=255) atatCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGAtctt > 2:2941688/1‑76 (MQ=255) atatCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGAtctt > 1:26082/1‑76 (MQ=255) tatCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCACCA‑GGCTACGCATTAAACGAGCGGAGCGAgcttc > 2:4474786/1‑76 (MQ=255) aatCGCCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGAtcttc < 1:1663571/75‑1 (MQ=255) gtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCC‑GGCTACGCATTAAATGAGCGGAGAGATCCTCTgca > 1:4195123/1‑76 (MQ=255) gtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgca < 1:3230413/76‑1 (MQ=255) gtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgca > 2:4302689/1‑76 (MQ=255) cgggggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg < 1:581912/73‑1 (MQ=255) cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg > 2:2488054/1‑76 (MQ=255) cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg < 2:2038616/76‑1 (MQ=255) cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg < 2:749526/76‑1 (MQ=255) cgAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAGCA‑GGCGACGCATTAAATGAGCGGAGAGATCTTCTgcacg > 1:338138/1‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc < 1:3657017/76‑1 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 1:795580/1‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 1:2714772/1‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 2:1260271/1‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 1:1271397/1‑76 (MQ=255) gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc > 2:888395/1‑76 (MQ=255) ggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGcacgcacg < 1:2941688/76‑1 (MQ=255) ccgccgCTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGtt < 2:3202628/76‑1 (MQ=255) cgccgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTa > 2:2803136/1‑76 (MQ=255) gccgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc > 1:3861075/1‑76 (MQ=255) gccgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc < 1:2710316/76‑1 (MQ=255) gccgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc > 1:1344330/1‑76 (MQ=255) gccgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc < 1:4639305/76‑1 (MQ=255) ccgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACg < 2:2482947/76‑1 (MQ=255) ccgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACg > 1:2502272/1‑76 (MQ=255) cgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTCCGa > 2:3767167/1‑76 (MQ=255) cgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGa > 1:3429483/1‑76 (MQ=255) cgcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGCTACGa > 1:3098703/1‑76 (MQ=255) gcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa > 1:1841578/1‑76 (MQ=255) gcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa > 1:3936427/1‑76 (MQ=255) gcTTCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa < 2:1327279/76‑1 (MQ=255) gcTTCCAGCGACGGGTTACGCTCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa < 1:1455438/76‑1 (MQ=255) gcTTACAGCGCCGGGGTACGCGCCACCA‑GGCTACGCATGAAATGAGCGGAGAGATCTTCTACACGCACCATACgta > 2:110568/1‑74 (MQ=255) cTTGCAGCTCCGGGTTATGCGCCAGCATGC‑TGCGCATTACCTGAGTGGCGAGCTCTTCTGCGCGCACGTTACGaaa < 1:3625756/76‑1 (MQ=255) ttCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaaa < 1:1824113/76‑1 (MQ=255) gtCCAGCGCAGGGATATGCGCCAGGA‑GGCTACGATTTAAATGAGCGGAGAGATAGTCTGCACGCATGCTGCGaaaa > 1:419564/2‑76 (MQ=255) tCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaaac > 2:45183/1‑75 (MQ=255) tCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAg > 1:517175/1‑76 (MQ=255) tCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAg < 1:2213707/76‑1 (MQ=255) tCCAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGACCGGAAAGATCTCCTCCACCCACGTGACGaaaac > 2:272961/1‑75 (MQ=255) tCCAGCGCCGGGTTACGCGCAAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAg < 1:2219162/76‑1 (MQ=255) ccAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGc < 1:4595987/76‑1 (MQ=255) cAGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCa < 1:2203512/76‑1 (MQ=255) aGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAc < 1:2149514/76‑1 (MQ=255) aGCGCCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAc > 2:2787378/1‑76 (MQ=255) cgcCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCg > 1:3829295/1‑76 (MQ=255) cgcCGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCg > 1:3187121/1‑76 (MQ=255) ccGGGTTACGCGCCAGCA‑GGCTACGCATTAAAGGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGcc < 2:3058065/76‑1 (MQ=255) cGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGccc > 2:490863/1‑76 (MQ=255) cGGGTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGccc > 1:1312474/1‑76 (MQ=255) gggTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt > 2:4808559/1‑76 (MQ=255) gggTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt < 2:4096617/76‑1 (MQ=255) gggTTACGCGCCAGCA‑GGCTACGCATGAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt > 1:586614/1‑76 (MQ=255) ggTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCtt < 1:66937/76‑1 (MQ=255) ggTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCtt < 1:4105667/76‑1 (MQ=255) gTTACTCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc < 1:1230274/76‑1 (MQ=255) gTTACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc < 1:323165/76‑1 (MQ=255) ttACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg > 2:4522225/1‑76 (MQ=255) ttACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg > 1:1620535/1‑76 (MQ=255) ttACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTGACGAAAAGCACCGCCCGTCg > 2:829035/1‑76 (MQ=255) tACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGa < 2:1714110/76‑1 (MQ=255) tACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGa > 1:2254599/1‑76 (MQ=255) tACGCGCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGa < 1:472931/76‑1 (MQ=255) gcgcgCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGaa < 2:908515/75‑1 (MQ=255) cgcgCCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAc < 1:3496454/76‑1 (MQ=255) gcgcCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACg > 2:3967269/1‑76 (MQ=255) gcgcCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACg > 2:1300513/1‑76 (MQ=255) gcgcCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACg > 1:3361108/1‑76 (MQ=255) gcgcCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACg > 1:2005326/1‑76 (MQ=255) cgcCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACgg < 1:4668628/76‑1 (MQ=255) gcCAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGc > 1:1887877/1‑76 (MQ=255) ccAGCA‑GGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGcc < 1:2152718/76‑1 (MQ=255) || CTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCA‑GCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC > NC_000913/4026183‑4026320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |