| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,194,577 | 2 bp→GC | coding (97‑98/774 nt) | yjaD → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,194,577 | 0 | C | G | 100.0% | 220.4 / NA | 84 | R33A (CGG→GCG) | yjaD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (51/33); total (51/33) | |||||||||||
| * | NC_000913 | 4,194,578 | 0 | G | C | 97.6% | 205.2 / NA | 83 | R33A (CGG→GCG) | yjaD | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base C (48/33); total (50/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAACGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG > NC_000913/4194507‑4194645 || tCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCa > 1:909920/1‑76 (MQ=255) cACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGGAGCaa > 1:4126419/1‑76 (MQ=255) aCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCCTATGGGGAAGCGGCaaa > 2:4517742/1‑76 (MQ=255) ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAtt < 1:567027/76‑1 (MQ=255) ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAtt > 2:3130002/1‑76 (MQ=255) ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAtt > 1:3105351/1‑76 (MQ=255) gCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAttt > 2:1653316/1‑76 (MQ=255) cTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTc > 1:576626/1‑76 (MQ=255) cTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTc < 1:363746/76‑1 (MQ=255) tggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCg > 2:1774006/1‑76 (MQ=255) tggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCg > 1:713128/1‑76 (MQ=255) tggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCg > 1:4212320/1‑76 (MQ=255) tggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCg < 2:3657933/76‑1 (MQ=255) gtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGAt > 1:4484411/1‑76 (MQ=255) gtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGAt < 1:3846063/76‑1 (MQ=255) tggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATc > 2:4050487/1‑76 (MQ=255) tggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATc > 1:999226/1‑76 (MQ=255) tcggTCGTCAGCCCTGAACAAAAATTATGGCTGCCCGGCGGAGAATTGCCATATGCCGAAGGGGCAAAGTTCGATc > 2:2558345/3‑76 (MQ=255) gggTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCGATATGGCGGAGCGGCAAATTTCGATCt > 1:2324291/1‑76 (MQ=255) ggTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCtt < 1:4301023/76‑1 (MQ=255) gtcgtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtg < 1:3782674/76‑1 (MQ=255) gtcgtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtg > 1:1556988/1‑76 (MQ=255) tcgtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgt < 1:497700/76‑1 (MQ=255) gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTgg < 1:3680477/76‑1 (MQ=255) gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTgg < 2:2801320/76‑1 (MQ=255) tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTggg > 2:3744513/1‑76 (MQ=255) cAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGt > 1:2261962/1‑76 (MQ=255) cAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGt < 2:2796173/76‑1 (MQ=255) aGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTc < 1:957314/76‑1 (MQ=255) aGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTc > 1:2968030/1‑76 (MQ=255) aGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTc < 2:2433560/76‑1 (MQ=255) gCCATGAACAAAAATTATGGTTGCCGCAGGGAGAATGGCTATATGGAGAAGCGGCAAATTTCGGTCTTGTGCGTCa > 2:4721683/1‑76 (MQ=255) gCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCa < 2:3285373/76‑1 (MQ=255) gCCATGAACAAAAATTATGGCTGCCGAAGGGAGAATTGCCATACGGCGTAGCGGCAGACGTCGATCCTGTGGGACa > 2:4623726/1‑76 (MQ=255) ccATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAg > 1:4076387/1‑76 (MQ=255) cATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgc < 2:3273926/76‑1 (MQ=255) cATGAACAAAAATTATGGGTGCCGAAGGGAGAATTGCCATATGGCGCAGCGGAAAATTTCGATCTTGTCGGTCAgc > 2:3192383/1‑76 (MQ=255) tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgc > 2:629259/1‑76 (MQ=255) tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgc < 2:3695545/76‑1 (MQ=255) tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgc < 1:2527087/76‑1 (MQ=255) aaCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcgc > 1:3606532/1‑76 (MQ=255) aaCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcgc < 2:263020/76‑1 (MQ=255) cAAAAATTATGGTTGCCGAAGGGAGAATCACCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCAc > 1:3879218/1‑76 (MQ=255) aaaaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACt > 2:4067652/1‑76 (MQ=255) aaaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAACTTTCGATCTTGTGGGTCAGCGCGCCCtc > 2:57896/1‑75 (MQ=255) aaaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTa > 1:9158/1‑76 (MQ=255) aaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTAc < 2:4558070/76‑1 (MQ=255) aaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTAc < 1:836585/76‑1 (MQ=255) aaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACa > 2:3668758/1‑76 (MQ=255) aTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACaa > 1:2245440/1‑76 (MQ=255) ttATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACaaa > 2:2327567/1‑76 (MQ=255) tATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAt > 1:3951196/1‑76 (MQ=255) tATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAt < 1:2344154/76‑1 (MQ=255) aTGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATc < 1:3793721/76‑1 (MQ=255) tGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAtag > 2:1301176/1‑74 (MQ=255) ggTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAACTTCGATCTTGTGGGTCCGCGCGCACTACAAATCgg > 2:992115/1‑76 (MQ=255) tGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGa > 2:752020/1‑76 (MQ=255) tGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGa > 1:4068503/1‑76 (MQ=255) gCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGaa < 2:3791360/76‑1 (MQ=255) ccGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAt > 2:2313187/1‑76 (MQ=255) ccGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAt < 1:3888600/76‑1 (MQ=255) cGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATg > 1:137479/1‑76 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg < 2:1655504/76‑1 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg > 1:1898082/1‑76 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg > 1:2083704/1‑76 (MQ=255) gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg > 2:4401738/1‑76 (MQ=255) aGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCa < 2:1184948/76‑1 (MQ=255) aGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCa < 1:763948/76‑1 (MQ=255) aGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCa > 2:2095586/1‑76 (MQ=255) ggAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAgg < 1:278915/76‑1 (MQ=255) gaagaCTTGCCATATGGCGAAGCGGCACATTAGGAACGTGTAGGTCAGCGCGCACTGCAAATCGGCGCATGGCAgg < 1:2558345/74‑1 (MQ=255) gagaATTGCCATATGGCGAAGCGGCAAATGTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggg > 2:1722101/1‑76 (MQ=255) agaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAgggg < 2:2485481/76‑1 (MQ=255) gaATTGCCATATGGCGAAGCGCCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggggg < 2:3254032/76‑1 (MQ=255) cacttGCCATACGGCGCAGCGGCAAATTTCGACCTTGTGGCCCAGCGCGCACTACACATCGGCGCATGTCAggggg > 2:180312/4‑76 (MQ=255) aaTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAACTCGGCGAATGGCAGGGGGa > 2:4729441/1‑76 (MQ=255) aTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGaa < 2:2810557/76‑1 (MQ=255) tGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAAcc < 1:4289328/76‑1 (MQ=255) ccATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCCGGGGGAACCTg > 2:706129/1‑76 (MQ=255) cATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGt > 2:2087945/1‑76 (MQ=255) tatGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGttt > 2:4423743/1‑76 (MQ=255) tatGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGttt > 1:1094906/1‑76 (MQ=255) atGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTg > 2:2928045/1‑76 (MQ=255) tGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTgg < 1:2744578/76‑1 (MQ=255) || TCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAACGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG > NC_000913/4194507‑4194645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |