| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,166,935 | 2 bp→CG | coding (312‑313/483 nt) | b3023 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,166,935 | 0 | G | C | 99.3% | 371.6 / NA | 142 | L105V (CTT→GTT) | b3023 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (80/61); total (80/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 3,166,936 | 0 | C | G | 99.3% | 363.6 / ‑5.0 | 141 | Q104H (CAG→CAC) | b3023 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (79/61); minor base A (1/0); total (80/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
Only 100 of 142 total aligned reads displayed.
GCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAGCTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATC > NC_000913/3166867‑3167005 || gccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCgg < 1:3488419/76‑1 (MQ=255)gccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCgg > 2:3841171/1‑76 (MQ=255)gccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCgg < 1:2266431/76‑1 (MQ=255) ccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCggg > 2:1852859/1‑76 (MQ=255) ccgcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCggg < 1:916578/76‑1 (MQ=255) cgattGCAGGCAGCCAGGGGCGAATGATGCCCCAGACAGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGc > 1:1207712/4‑76 (MQ=255) gcTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGcc < 2:689571/76‑1 (MQ=255) cTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGGGGCGGGccc > 2:704459/1‑75 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAc > 1:3130910/1‑76 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAc > 2:4451753/1‑76 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAc < 1:450067/76‑1 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAc > 2:4683987/1‑76 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAc > 1:3281839/1‑76 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCCCCCCAACGTGGCGGGCCAc > 2:1298664/1‑76 (MQ=255) ttGCAGGCAGCCAGTGGCGAATGATGCCCCATACC‑‑GTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACgg > 1:2690404/1‑76 (MQ=255) tGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACg > 2:4536614/1‑76 (MQ=255) ggcaggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACg < 2:1834061/75‑1 (MQ=255) gcaggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACgg > 1:3745027/1‑76 (MQ=255) gcaggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACgg > 2:2073348/1‑76 (MQ=255) gcaggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACgg > 1:2869387/1‑76 (MQ=255) gcaggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCCCCCACGTGGCGGCCCACgc > 2:596836/1‑75 (MQ=255) caggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGc > 2:553707/1‑76 (MQ=255) aggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCa > 1:3502718/1‑76 (MQ=255) aggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCa < 1:1501865/76‑1 (MQ=255) aggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCa > 1:4050046/1‑76 (MQ=255) ggcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCAt < 2:4050046/76‑1 (MQ=255) gcagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATa > 1:544021/1‑76 (MQ=255) cagCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATac > 2:3388604/1‑75 (MQ=255) gCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACg < 1:4031321/76‑1 (MQ=255) gCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACg < 2:1083499/76‑1 (MQ=255) ccAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGt > 1:2796646/1‑76 (MQ=255) ccAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGGGGCGGGCCACGGCATAACgc > 2:226182/1‑75 (MQ=255) gaGTGGCGAATGGTGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAGCGTc < 2:546996/75‑1 (MQ=255) cAGTGGCGAATGATGCCCCATACCGTGTGTGAAATTTCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTc < 2:111828/76‑1 (MQ=255) cAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTc > 1:482312/1‑76 (MQ=255) aGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTcc < 1:2955725/76‑1 (MQ=255) tGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTccca > 1:1108332/1‑74 (MQ=255) tGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAc > 1:3039153/1‑76 (MQ=255) gggCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAc < 1:4591234/75‑1 (MQ=255) ggCGAATGATGCCGCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAcc < 1:3953188/76‑1 (MQ=255) ggCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAcc < 1:456068/76‑1 (MQ=255) gCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCg > 2:214128/1‑76 (MQ=255) cGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCgg > 1:2127429/1‑76 (MQ=255) cGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCgg > 1:2549203/1‑76 (MQ=255) gAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCgga > 2:833076/1‑75 (MQ=255) gAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCgga > 2:3722763/1‑75 (MQ=255) aaTGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTa > 1:1760668/1‑76 (MQ=255) atgatgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTaa > 2:1823351/1‑76 (MQ=255) atgatgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTaa > 1:2432400/1‑76 (MQ=255) tgatgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAg < 1:2752958/76‑1 (MQ=255) tgatgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAg < 1:2900881/76‑1 (MQ=255) atgCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGtt > 1:4346369/1‑76 (MQ=255) cccATCCCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAca < 2:4447715/76‑2 (MQ=255) cccATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAcc < 1:929461/76‑1 (MQ=255) ccATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCa > 1:1449464/1‑76 (MQ=255) cATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGCTCACCAt > 2:4727103/1‑76 (MQ=255) cATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGCCCACCGGTAAGTACACCAt > 2:4102112/1‑76 (MQ=255) aTACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAtt < 2:3850906/76‑1 (MQ=255) tACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTg < 1:581284/76‑1 (MQ=255) tACCGTGTGTGAAATATCGTCCAGCTCGCCAACATGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTg > 2:2668010/1‑76 (MQ=255) aCCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGc > 2:1381593/1‑76 (MQ=255) aCCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAACATTGc > 2:2262817/1‑76 (MQ=255) aCCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGGTCAACAtttc > 1:1853342/1‑74 (MQ=255) ccGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCt < 2:1923963/76‑1 (MQ=255) ccGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCt > 2:4351440/1‑76 (MQ=255) ccGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCt < 1:1850565/76‑1 (MQ=255) ccGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGCAAGTTCCCCATTGcc > 2:3363437/1‑75 (MQ=255) tgtgggAAATCTGGTCCAGCTCGCGAACGTGGCGGTCCGCGGCGTAACGTCCACCGGTAAGCTCACCATTGCTccc < 2:3560860/76‑3 (MQ=255) gtgtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTcaca > 2:1475738/1‑76 (MQ=255) gggtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTcaca < 2:2103053/74‑1 (MQ=255) gcgtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCAGACAGGCCACCGCTAAGTTCACCATTGCTcaca > 2:1951939/3‑76 (MQ=255) tgtgGAATATCTGCCAGCTCTCCAACGTGGCGTGCCACGGCATAACGTCCACCGGTAAGTTCCCCATTGCTCacac < 2:1448396/76‑1 (MQ=255) tgtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCacac < 2:2962191/76‑1 (MQ=255) gtgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACAcc > 1:635957/1‑76 (MQ=255) tgAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCa > 1:3130878/1‑76 (MQ=255) gAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCAt < 1:214128/76‑1 (MQ=255) gAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGCCCACCGCTAAGGTCACCATTGCTCACAccca > 2:913999/1‑74 (MQ=255) gAAATAGCGCCCAGCCCGCCAACGTGGCGGGCCGCGGCAGAACGTCCACCGGTAAGTTCACCATTGCTCACACCAt < 2:1637227/76‑1 (MQ=255) aaaTATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATa > 2:3054910/1‑76 (MQ=255) aaTATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATaa < 1:2881375/76‑1 (MQ=255) atatCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAAc < 2:3226731/76‑1 (MQ=255) atatCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAAc < 1:2335424/76‑1 (MQ=255) atatCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAAc < 1:3544151/76‑1 (MQ=255) atCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGa < 1:1192668/76‑1 (MQ=255) tCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAt < 1:2632750/76‑1 (MQ=255) cctCCACCCCGCCCACCTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAtt < 1:375264/74‑1 (MQ=255) cGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAtt < 1:4451753/76‑1 (MQ=255) cGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAtt > 1:4222503/1‑76 (MQ=255) cGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAtt > 2:3643504/1‑76 (MQ=255) gTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTa > 1:4676587/1‑76 (MQ=255) gTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTa > 1:2616767/1‑76 (MQ=255) ccAGCTCGCCAACGTGGCGGGCCACGGCATAACCGCCACCGGGAAGTTCACCATTGCTCACACCATAACGATTacc > 1:1551835/1‑74 (MQ=255) cAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCg < 1:581127/76‑1 (MQ=255) aGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCgg < 2:4545758/76‑1 (MQ=255) aGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCgg > 1:1558195/1‑76 (MQ=255) aGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCgg < 2:2723278/76‑1 (MQ=255) cTCGCCAGGGTGGCGGGCCACGGCATGACGTCCACCGGTAAGTTCACCATTGCTCGCACCATAACGATTATCGGGa < 2:1723638/76‑1 (MQ=255) cTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGCTTATCGGGa > 2:1242343/1‑76 (MQ=255) tCGCCAACGTGGCGGGGCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGaa > 1:2256771/1‑76 (MQ=255) tCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGaa < 1:3623359/76‑1 (MQ=255) || GCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAGCTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATC > NC_000913/3166867‑3167005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |