| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,221,097 | 2 bp→GC | coding (824‑825/3093 nt) | b3076 → | evolved beta‑D‑galactosidase alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,221,097 | 0 | C | G | 100.0% | 324.9 / NA | 130 | T275S (ACG→AGC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (67/63); total (67/63) | |||||||||||
| * | NC_000913 | 3,221,098 | 0 | G | C | 99.2% | 320.4 / NA | 131 | T275S (ACG→AGC) | b3076 | evolved beta‑D‑galactosidase alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (66/63); minor base A (1/0); total (67/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 132 total aligned reads displayed.
TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG > NC_000913/3221028‑3221167 || ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGc > 1:467670/1‑76 (MQ=255) tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCCGCTTTGCt > 2:3878888/1‑76 (MQ=255) gATGGCGAGCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt < 2:1660829/76‑1 (MQ=255) gATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt < 1:1285609/76‑1 (MQ=255) tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCCGCTTTGCtttt > 2:359337/1‑76 (MQ=255) tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtttt > 1:3972131/1‑76 (MQ=255) tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtttt < 1:3311510/76‑1 (MQ=255) ggCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTa < 1:1768352/76‑1 (MQ=255) ggCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTa > 1:3796426/1‑76 (MQ=255) ggCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTa > 2:4121037/1‑76 (MQ=255) gCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAc > 2:1831548/1‑76 (MQ=255) gCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAc > 1:4681925/1‑76 (MQ=255) caaaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACt < 1:4207114/74‑1 (MQ=255) cGAACGCGTGGTGCACAGCAGCGCCATTGCTCATTTGGCAATTGAAAAACTGACCATCGCCAGCTTTGCTTTTACt > 1:2412003/1‑76 (MQ=255) cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACt < 2:2841075/76‑1 (MQ=255) gAACGCGTGGTGCACAGCAGCGCCATTGTTCATTAGGCAATTGAAAAACTGACCCGCGCCCGCTTTCCTTTTGCTg > 2:4228766/1‑76 (MQ=255) gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg > 2:3497580/1‑76 (MQ=255) gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg > 2:4062549/1‑76 (MQ=255) gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg > 1:3250199/1‑76 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCGATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt < 1:649776/76‑1 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTgc > 2:1422903/1‑75 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt < 1:3709135/76‑1 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt > 1:1436614/1‑76 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt < 2:2332398/76‑1 (MQ=255) aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt < 1:4835079/76‑1 (MQ=255) cgcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCg > 1:624186/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGa > 1:2826151/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGa > 1:4687847/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGa > 1:2372990/1‑76 (MQ=255) gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGATTTTACTGTCGa > 2:1656358/1‑76 (MQ=255) cgTGGTGCACAGCAGCGCCATTGATCACTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGaa > 2:1091175/1‑76 (MQ=255) tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACa > 2:1286069/1‑76 (MQ=255) tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACa < 1:4161507/76‑1 (MQ=255) tggtgCACAGCAGCGCCATTGATCATGTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACa < 2:2900493/76‑1 (MQ=255) ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAg < 1:4720695/76‑1 (MQ=255) gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc < 2:3702635/76‑1 (MQ=255) gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc > 2:622166/1‑76 (MQ=255) tgCACAGCAGCGCCATTGATCATTTTGCAATTGAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc > 1:2657269/1‑76 (MQ=255) tgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc > 1:1487305/1‑76 (MQ=255) gcccagcagCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg < 2:1171321/73‑1 (MQ=255) gCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg < 1:4227158/76‑1 (MQ=255) gCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg > 1:2420290/1‑76 (MQ=255) cacaGCGGCGCCATTGATCATTTGGCAATTGAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc < 2:1921205/76‑1 (MQ=255) acaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgca < 2:3220891/76‑1 (MQ=255) aaagcagcGACATTGATCAGTTGGCTATTGAAAAACTGACCAGCGCCAGCGTTGCTTTTAGTGGCGAAGAGCCgca > 1:2047511/3‑76 (MQ=255) cagcagCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcag < 1:1878846/76‑1 (MQ=255) agcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcagc > 2:3590693/1‑76 (MQ=255) gcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgtagca > 2:2614006/1‑76 (MQ=255) cagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa > 1:1731176/1‑76 (MQ=255) cagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa > 2:2216445/1‑76 (MQ=255) agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCAAACAGCCGCAGCAAt < 2:2074003/76‑1 (MQ=255) gcgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCCATg > 2:224002/1‑76 (MQ=255) gcgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATg < 2:3983233/76‑1 (MQ=255) gcgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATg > 2:3342893/1‑76 (MQ=255) gcgcCATTGATCATTCGGCAATTGAAAACCTGACCAGCGCCAGCTTTGCTGTTACGGTCGACCAGTCGCAGGAATg > 2:4670144/1‑76 (MQ=255) cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATgg > 1:680570/1‑76 (MQ=255) cgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATgg > 2:788967/1‑76 (MQ=255) gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt > 1:2096403/1‑76 (MQ=255) gcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt > 1:3593258/1‑76 (MQ=255) ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc > 1:869935/1‑76 (MQ=255) ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc > 1:1208239/1‑76 (MQ=255) ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc > 1:387750/1‑76 (MQ=255) cATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca > 2:3435273/1‑76 (MQ=255) taaTCATTGGACAATGGAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTcagca < 1:173017/74‑1 (MQ=255) gATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTCGCTTTTACTGTCGAACAGCCGCAGCAATGGTccgcag > 2:2836180/1‑76 (MQ=255) aTCATTTGGCAATTAAAAAACTGACCAGCGCAAGCTTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGa < 2:2282565/76‑1 (MQ=255) tCATTTGGCAATTGAAAACCTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa < 1:1573544/76‑1 (MQ=255) tCATTTGGCAATTGAAAACCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa < 1:1922820/76‑1 (MQ=255) tCATTGGGCAATTGAAAACCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa < 2:3512231/76‑1 (MQ=255) cATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt < 2:2420852/76‑1 (MQ=255) cATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt < 2:1387507/76‑1 (MQ=255) cATTGGGCAATTGAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt < 1:850066/76‑1 (MQ=255) aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATc > 1:3951972/1‑76 (MQ=255) tttGGCAATTAAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcc < 2:3175762/76‑1 (MQ=255) ggCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt < 2:3909868/76‑1 (MQ=255) ggCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt > 1:3942082/1‑76 (MQ=255) gCATTTGAAAAACTGCCCAGCGCCAGCTTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCtt < 1:4561181/76‑1 (MQ=255) gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt < 1:4056503/76‑1 (MQ=255) gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt > 1:341689/1‑76 (MQ=255) aaaTTGAAAAACGGCCCAGCGCCAGTTTTGTTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa < 2:1731175/75‑1 (MQ=255) aTTGAAAACCCGCCCAGCCCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATc < 2:341689/76‑1 (MQ=255) ttGAAAACCTGCCCGGCGCCAGCTTTGCTTTTACTGCCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCt < 1:1963358/76‑1 (MQ=255) ttGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCt < 1:1388521/76‑1 (MQ=255) taaaaacCTGCCCAGCGCCAGCTTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCtt < 1:891849/74‑1 (MQ=255) tGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCtt > 2:3481433/1‑76 (MQ=255) gAAAACCTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCttt < 1:2637911/76‑1 (MQ=255) gAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCttt > 2:1176724/1‑76 (MQ=255) aaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc > 1:798557/1‑76 (MQ=255) aaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc > 1:2104418/1‑76 (MQ=255) accTGCCCAGCGCCAGCTTTGCTTTTACTGTCGACCAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCa < 1:1190740/74‑1 (MQ=255) aaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCa > 1:2812184/1‑76 (MQ=255) aaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCa < 2:833580/76‑1 (MQ=255) aCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt > 1:4534978/1‑76 (MQ=255) cTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATc > 2:818373/1‑76 (MQ=255) gACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg > 1:2863516/1‑76 (MQ=255) aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg > 2:3060637/1‑76 (MQ=255) cAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc > 1:3723938/1‑76 (MQ=255) aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa > 2:1850524/1‑76 (MQ=255) aGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa < 1:2581881/76‑1 (MQ=255) gcgcCAGCTTTGCTTTGACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAt < 2:544367/76‑1 (MQ=255) || TTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATG > NC_000913/3221028‑3221167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |